A standardized surgical technique for rat superior cervical ganglionectomy
- Autores
- Savastano, Luis Emilio; Castro Zapata, Analia Elizabeth; Fitt, Marcos René; Rath, Martin Fredensborg; Romeo, Horacio Eduardo; Muñoz, Estela Maris
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-stepdescription of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and aftersurgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied.
Fil: Savastano, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Castro Zapata, Analia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Fitt, Marcos René. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Rath, Martin Fredensborg. Universidad de Copenhagen; Dinamarca
Fil: Romeo, Horacio Eduardo. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina - Materia
-
SCG
SCGx
DECENTRALIZATION
DENERVATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/243443
Ver los metadatos del registro completo
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A standardized surgical technique for rat superior cervical ganglionectomySavastano, Luis EmilioCastro Zapata, Analia ElizabethFitt, Marcos RenéRath, Martin FredensborgRomeo, Horacio EduardoMuñoz, Estela MarisSCGSCGxDECENTRALIZATIONDENERVATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-stepdescription of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and aftersurgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied.Fil: Savastano, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Castro Zapata, Analia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Fitt, Marcos René. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Rath, Martin Fredensborg. Universidad de Copenhagen; DinamarcaFil: Romeo, Horacio Eduardo. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaElsevier Science2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243443Savastano, Luis Emilio; Castro Zapata, Analia Elizabeth; Fitt, Marcos René; Rath, Martin Fredensborg; Romeo, Horacio Eduardo; et al.; A standardized surgical technique for rat superior cervical ganglionectomy; Elsevier Science; Journal of Neuroscience Methods; 192; 1; 9-2010; 22-330165-0270CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165027010003614info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneumeth.2010.07.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:37Zoai:ri.conicet.gov.ar:11336/243443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:37.925CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
A standardized surgical technique for rat superior cervical ganglionectomy |
| title |
A standardized surgical technique for rat superior cervical ganglionectomy |
| spellingShingle |
A standardized surgical technique for rat superior cervical ganglionectomy Savastano, Luis Emilio SCG SCGx DECENTRALIZATION DENERVATION |
| title_short |
A standardized surgical technique for rat superior cervical ganglionectomy |
| title_full |
A standardized surgical technique for rat superior cervical ganglionectomy |
| title_fullStr |
A standardized surgical technique for rat superior cervical ganglionectomy |
| title_full_unstemmed |
A standardized surgical technique for rat superior cervical ganglionectomy |
| title_sort |
A standardized surgical technique for rat superior cervical ganglionectomy |
| dc.creator.none.fl_str_mv |
Savastano, Luis Emilio Castro Zapata, Analia Elizabeth Fitt, Marcos René Rath, Martin Fredensborg Romeo, Horacio Eduardo Muñoz, Estela Maris |
| author |
Savastano, Luis Emilio |
| author_facet |
Savastano, Luis Emilio Castro Zapata, Analia Elizabeth Fitt, Marcos René Rath, Martin Fredensborg Romeo, Horacio Eduardo Muñoz, Estela Maris |
| author_role |
author |
| author2 |
Castro Zapata, Analia Elizabeth Fitt, Marcos René Rath, Martin Fredensborg Romeo, Horacio Eduardo Muñoz, Estela Maris |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
SCG SCGx DECENTRALIZATION DENERVATION |
| topic |
SCG SCGx DECENTRALIZATION DENERVATION |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-stepdescription of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and aftersurgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied. Fil: Savastano, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Castro Zapata, Analia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Fitt, Marcos René. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Rath, Martin Fredensborg. Universidad de Copenhagen; Dinamarca Fil: Romeo, Horacio Eduardo. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina |
| description |
Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-stepdescription of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and aftersurgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010-09 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/243443 Savastano, Luis Emilio; Castro Zapata, Analia Elizabeth; Fitt, Marcos René; Rath, Martin Fredensborg; Romeo, Horacio Eduardo; et al.; A standardized surgical technique for rat superior cervical ganglionectomy; Elsevier Science; Journal of Neuroscience Methods; 192; 1; 9-2010; 22-33 0165-0270 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/243443 |
| identifier_str_mv |
Savastano, Luis Emilio; Castro Zapata, Analia Elizabeth; Fitt, Marcos René; Rath, Martin Fredensborg; Romeo, Horacio Eduardo; et al.; A standardized surgical technique for rat superior cervical ganglionectomy; Elsevier Science; Journal of Neuroscience Methods; 192; 1; 9-2010; 22-33 0165-0270 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165027010003614 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneumeth.2010.07.007 |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf |
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Elsevier Science |
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Elsevier Science |
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