In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC

Autores
Díaz, Jorge Daniel; Corrada, Yanina Alejandra; Blanco, Paula Graciela; Gobello, María Cristina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aims of this study were to assess and compare the effect of skim milk with (MEY) and without (SMI) egg yolk on canine spermatozoa incubated at 4ºC in vitro and to evaluate the efficacy of MEY in vivo. Also, the effect of semen cooled storage before freezing was also evaluated in vitro. The ejaculates of 10 dogs were collected, pooled, centrifuged, and divided into 4 aliquots and diluted in one of the following 4 diluents: Prostatic fluid (PRO), commercial diluent (COM), SMI, or MEY. Extended samples were stored at 4°C and evaluated daily for 6 days. Percentage of total (P < 0.01) and progressive (P < 0.01) motility, intact acrosomes (P < 0.05), and positive endosmosis (P < 0.01) decreased over time in the diluents, with COM and PRO having as the best and the worst performances, respectively. Furthermore, MEY motility differed from PRO (P < 0.01) and SMI (P < 0.01) but not COM. Acrosome integrity was higher in MEY when compared to SMI (P < 0.05). Sixteen pairs of dogs were randomly assigned to either fresh undiluted semen (n = 8) or to 24-h cooled MEY diluted semen (n = 8). Seven (87.5%; P > 0.1) bitches from each group became pregnant and whelped normally. MEY extended semen samples were cooled for 2, 24, or 48 h at 4°C, and a second dilution was performed prior to freezing and thawing. Post-thaw total and forward sperm motility decreased with increasing cooled storage (P < 0.05), although no significant differences in total or forward motility, normal acrosomes, positive endosmosis, live spermatozoa, or positive endosmosis were found between 2 and 24 h storage. These in vitro and in vivo results show that MEY can be considered a simple, inexpensive, and efficient diluent for canine semen chilling. Furthermore, MEY could be successfully frozen after 1 day of cooled storage.
Fil: Díaz, Jorge Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Blanco, Paula Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Gobello, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
ARTIFICIAL INSEMINATION
COOLED SEMEN
DOG
FROZEN SEMEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14427
Acceder
id CONICETDig_11265ac6af769516db108b04de421f62
oai_identifier_str oai:ri.conicet.gov.ar:11336/14427
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºCDíaz, Jorge DanielCorrada, Yanina AlejandraBlanco, Paula GracielaGobello, María CristinaARTIFICIAL INSEMINATIONCOOLED SEMENDOGFROZEN SEMENhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aims of this study were to assess and compare the effect of skim milk with (MEY) and without (SMI) egg yolk on canine spermatozoa incubated at 4ºC in vitro and to evaluate the efficacy of MEY in vivo. Also, the effect of semen cooled storage before freezing was also evaluated in vitro. The ejaculates of 10 dogs were collected, pooled, centrifuged, and divided into 4 aliquots and diluted in one of the following 4 diluents: Prostatic fluid (PRO), commercial diluent (COM), SMI, or MEY. Extended samples were stored at 4°C and evaluated daily for 6 days. Percentage of total (P < 0.01) and progressive (P < 0.01) motility, intact acrosomes (P < 0.05), and positive endosmosis (P < 0.01) decreased over time in the diluents, with COM and PRO having as the best and the worst performances, respectively. Furthermore, MEY motility differed from PRO (P < 0.01) and SMI (P < 0.01) but not COM. Acrosome integrity was higher in MEY when compared to SMI (P < 0.05). Sixteen pairs of dogs were randomly assigned to either fresh undiluted semen (n = 8) or to 24-h cooled MEY diluted semen (n = 8). Seven (87.5%; P > 0.1) bitches from each group became pregnant and whelped normally. MEY extended semen samples were cooled for 2, 24, or 48 h at 4°C, and a second dilution was performed prior to freezing and thawing. Post-thaw total and forward sperm motility decreased with increasing cooled storage (P < 0.05), although no significant differences in total or forward motility, normal acrosomes, positive endosmosis, live spermatozoa, or positive endosmosis were found between 2 and 24 h storage. These in vitro and in vivo results show that MEY can be considered a simple, inexpensive, and efficient diluent for canine semen chilling. Furthermore, MEY could be successfully frozen after 1 day of cooled storage.Fil: Díaz, Jorge Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Blanco, Paula Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Gobello, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaColégio Brasileiro de Reprodução Animal2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14427Díaz, Jorge Daniel; Corrada, Yanina Alejandra; Blanco, Paula Graciela; Gobello, María Cristina; In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC; Colégio Brasileiro de Reprodução Animal; Revista Brasileira de Reprodução Animal; 10; 4; 10-2013; 670-6761806-96141984-3143enginfo:eu-repo/semantics/altIdentifier/url/http://pesquisa.bvsalud.org/bvsvs/resource/pt/vti-9892info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:55Zoai:ri.conicet.gov.ar:11336/14427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:56.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
title In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
spellingShingle In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
Díaz, Jorge Daniel
ARTIFICIAL INSEMINATION
COOLED SEMEN
DOG
FROZEN SEMEN
title_short In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
title_full In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
title_fullStr In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
title_full_unstemmed In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
title_sort In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC
dc.creator.none.fl_str_mv Díaz, Jorge Daniel
Corrada, Yanina Alejandra
Blanco, Paula Graciela
Gobello, María Cristina
author Díaz, Jorge Daniel
author_facet Díaz, Jorge Daniel
Corrada, Yanina Alejandra
Blanco, Paula Graciela
Gobello, María Cristina
author_role author
author2 Corrada, Yanina Alejandra
Blanco, Paula Graciela
Gobello, María Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv ARTIFICIAL INSEMINATION
COOLED SEMEN
DOG
FROZEN SEMEN
topic ARTIFICIAL INSEMINATION
COOLED SEMEN
DOG
FROZEN SEMEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aims of this study were to assess and compare the effect of skim milk with (MEY) and without (SMI) egg yolk on canine spermatozoa incubated at 4ºC in vitro and to evaluate the efficacy of MEY in vivo. Also, the effect of semen cooled storage before freezing was also evaluated in vitro. The ejaculates of 10 dogs were collected, pooled, centrifuged, and divided into 4 aliquots and diluted in one of the following 4 diluents: Prostatic fluid (PRO), commercial diluent (COM), SMI, or MEY. Extended samples were stored at 4°C and evaluated daily for 6 days. Percentage of total (P < 0.01) and progressive (P < 0.01) motility, intact acrosomes (P < 0.05), and positive endosmosis (P < 0.01) decreased over time in the diluents, with COM and PRO having as the best and the worst performances, respectively. Furthermore, MEY motility differed from PRO (P < 0.01) and SMI (P < 0.01) but not COM. Acrosome integrity was higher in MEY when compared to SMI (P < 0.05). Sixteen pairs of dogs were randomly assigned to either fresh undiluted semen (n = 8) or to 24-h cooled MEY diluted semen (n = 8). Seven (87.5%; P > 0.1) bitches from each group became pregnant and whelped normally. MEY extended semen samples were cooled for 2, 24, or 48 h at 4°C, and a second dilution was performed prior to freezing and thawing. Post-thaw total and forward sperm motility decreased with increasing cooled storage (P < 0.05), although no significant differences in total or forward motility, normal acrosomes, positive endosmosis, live spermatozoa, or positive endosmosis were found between 2 and 24 h storage. These in vitro and in vivo results show that MEY can be considered a simple, inexpensive, and efficient diluent for canine semen chilling. Furthermore, MEY could be successfully frozen after 1 day of cooled storage.
Fil: Díaz, Jorge Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Blanco, Paula Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Gobello, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description The aims of this study were to assess and compare the effect of skim milk with (MEY) and without (SMI) egg yolk on canine spermatozoa incubated at 4ºC in vitro and to evaluate the efficacy of MEY in vivo. Also, the effect of semen cooled storage before freezing was also evaluated in vitro. The ejaculates of 10 dogs were collected, pooled, centrifuged, and divided into 4 aliquots and diluted in one of the following 4 diluents: Prostatic fluid (PRO), commercial diluent (COM), SMI, or MEY. Extended samples were stored at 4°C and evaluated daily for 6 days. Percentage of total (P < 0.01) and progressive (P < 0.01) motility, intact acrosomes (P < 0.05), and positive endosmosis (P < 0.01) decreased over time in the diluents, with COM and PRO having as the best and the worst performances, respectively. Furthermore, MEY motility differed from PRO (P < 0.01) and SMI (P < 0.01) but not COM. Acrosome integrity was higher in MEY when compared to SMI (P < 0.05). Sixteen pairs of dogs were randomly assigned to either fresh undiluted semen (n = 8) or to 24-h cooled MEY diluted semen (n = 8). Seven (87.5%; P > 0.1) bitches from each group became pregnant and whelped normally. MEY extended semen samples were cooled for 2, 24, or 48 h at 4°C, and a second dilution was performed prior to freezing and thawing. Post-thaw total and forward sperm motility decreased with increasing cooled storage (P < 0.05), although no significant differences in total or forward motility, normal acrosomes, positive endosmosis, live spermatozoa, or positive endosmosis were found between 2 and 24 h storage. These in vitro and in vivo results show that MEY can be considered a simple, inexpensive, and efficient diluent for canine semen chilling. Furthermore, MEY could be successfully frozen after 1 day of cooled storage.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14427
Díaz, Jorge Daniel; Corrada, Yanina Alejandra; Blanco, Paula Graciela; Gobello, María Cristina; In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC; Colégio Brasileiro de Reprodução Animal; Revista Brasileira de Reprodução Animal; 10; 4; 10-2013; 670-676
1806-9614
1984-3143
url http://hdl.handle.net/11336/14427
identifier_str_mv Díaz, Jorge Daniel; Corrada, Yanina Alejandra; Blanco, Paula Graciela; Gobello, María Cristina; In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4ºC; Colégio Brasileiro de Reprodução Animal; Revista Brasileira de Reprodução Animal; 10; 4; 10-2013; 670-676
1806-9614
1984-3143
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pesquisa.bvsalud.org/bvsvs/resource/pt/vti-9892
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Reprodução Animal
publisher.none.fl_str_mv Colégio Brasileiro de Reprodução Animal
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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