Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876
- Autores
- Cavello, Ivana Alejandra; Cavalitto, Sebastian Fernando; Hours, Roque Alberto
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Enzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films.
Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina - Materia
-
Silver Recovery
Serine Proteases
X-Ray Films
Thermostability - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4325
Ver los metadatos del registro completo
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Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876Cavello, Ivana AlejandraCavalitto, Sebastian FernandoHours, Roque AlbertoSilver RecoverySerine ProteasesX-Ray FilmsThermostabilityhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Enzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaKorean Society Microbiology2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4325Cavello, Ivana Alejandra; Cavalitto, Sebastian Fernando; Hours, Roque Alberto; Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876; Korean Society Microbiology; Journal of Microbiology and Biotechnology; 23; 8; 6-2013; 1133-11391017-7825enginfo:eu-repo/semantics/altIdentifier/url/https://www.jmb.or.kr/journal/view.html?volume=23&number=8&spage=1133info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:50Zoai:ri.conicet.gov.ar:11336/4325instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:50.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
title |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
spellingShingle |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 Cavello, Ivana Alejandra Silver Recovery Serine Proteases X-Ray Films Thermostability |
title_short |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
title_full |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
title_fullStr |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
title_full_unstemmed |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
title_sort |
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876 |
dc.creator.none.fl_str_mv |
Cavello, Ivana Alejandra Cavalitto, Sebastian Fernando Hours, Roque Alberto |
author |
Cavello, Ivana Alejandra |
author_facet |
Cavello, Ivana Alejandra Cavalitto, Sebastian Fernando Hours, Roque Alberto |
author_role |
author |
author2 |
Cavalitto, Sebastian Fernando Hours, Roque Alberto |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Silver Recovery Serine Proteases X-Ray Films Thermostability |
topic |
Silver Recovery Serine Proteases X-Ray Films Thermostability |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Enzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films. Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina |
description |
Enzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4325 Cavello, Ivana Alejandra; Cavalitto, Sebastian Fernando; Hours, Roque Alberto; Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876; Korean Society Microbiology; Journal of Microbiology and Biotechnology; 23; 8; 6-2013; 1133-1139 1017-7825 |
url |
http://hdl.handle.net/11336/4325 |
identifier_str_mv |
Cavello, Ivana Alejandra; Cavalitto, Sebastian Fernando; Hours, Roque Alberto; Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876; Korean Society Microbiology; Journal of Microbiology and Biotechnology; 23; 8; 6-2013; 1133-1139 1017-7825 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.jmb.or.kr/journal/view.html?volume=23&number=8&spage=1133 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Korean Society Microbiology |
publisher.none.fl_str_mv |
Korean Society Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613461886107648 |
score |
13.070432 |