Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films

Autores
Velazquez, Diego Ezequiel; Latorre, Maria Emilia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Animal tissue by-products, rich in collagen, represent a valuable source of biomaterials. Understanding their physicochemical and thermal behavior is essential for expanding their applications. In this study, pigskin gelatin was extracted through acid hydrolysis using a combination of acetic acid (AH) and either lactic, citric, or ascorbic acid (75:25, v:v, [0.5 M]), followed by thermal denaturation. We evaluated the physicochemical properties of the gelatin solutions (pH, hydroxyproline content, and extraction yield), as well as the macroscopic gel characteristics. Gelatin films were then prepared and analyzed for moisture content, color, and thermal properties. One-way ANOVA was applied to compare treatments, and Pearson’s correlation was used to assess the relationship between the solution pH and physicochemical parameters. Significant differences in the final pH of the solutions were observed among the acid mixture treatments, though the hydroxyproline content and extraction yield were not significantly affected. All gelatin solutions formed stable gels, and the resulting films exhibited similar moisture content. Thermal analysis revealed treatmentdependent variations. Specifically, a significant negative correlation (p < 0.005) was found between the gelatin solution pH and the melting temperature. These results suggest that the use of organic acid mixtures can effectively modulate gelatin properties, offering a versatile approach for tailoring biomaterials for both food and non-food applications.
Fil: Velazquez, Diego Ezequiel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto de Física de Materiales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires | Universidad Nacional del Centro de la Pcia. de Bs.as. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
Fil: Latorre, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires | Universidad Nacional del Centro de la Pcia. de Bs.as. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
Materia
BIOMATERIALS
PROTEIN BIOPOLYMERS
DSC
ACID HYDROLYSIS
SKIN TISSUE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272465
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272465
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting FilmsVelazquez, Diego EzequielLatorre, Maria EmiliaBIOMATERIALSPROTEIN BIOPOLYMERSDSCACID HYDROLYSISSKIN TISSUEhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Animal tissue by-products, rich in collagen, represent a valuable source of biomaterials. Understanding their physicochemical and thermal behavior is essential for expanding their applications. In this study, pigskin gelatin was extracted through acid hydrolysis using a combination of acetic acid (AH) and either lactic, citric, or ascorbic acid (75:25, v:v, [0.5 M]), followed by thermal denaturation. We evaluated the physicochemical properties of the gelatin solutions (pH, hydroxyproline content, and extraction yield), as well as the macroscopic gel characteristics. Gelatin films were then prepared and analyzed for moisture content, color, and thermal properties. One-way ANOVA was applied to compare treatments, and Pearson’s correlation was used to assess the relationship between the solution pH and physicochemical parameters. Significant differences in the final pH of the solutions were observed among the acid mixture treatments, though the hydroxyproline content and extraction yield were not significantly affected. All gelatin solutions formed stable gels, and the resulting films exhibited similar moisture content. Thermal analysis revealed treatmentdependent variations. Specifically, a significant negative correlation (p < 0.005) was found between the gelatin solution pH and the melting temperature. These results suggest that the use of organic acid mixtures can effectively modulate gelatin properties, offering a versatile approach for tailoring biomaterials for both food and non-food applications.Fil: Velazquez, Diego Ezequiel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto de Física de Materiales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires | Universidad Nacional del Centro de la Pcia. de Bs.as. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Latorre, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires | Universidad Nacional del Centro de la Pcia. de Bs.as. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaMultidisciplinary Digital Publishing Institute2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272465Velazquez, Diego Ezequiel; Latorre, Maria Emilia; Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films; Multidisciplinary Digital Publishing Institute; Physchem; 5; 3; 9-2025; 1-122673-7167CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7167/5/3/38info:eu-repo/semantics/altIdentifier/doi/10.3390/physchem5030038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:16:03Zoai:ri.conicet.gov.ar:11336/272465instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:16:03.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
title Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
spellingShingle Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
Velazquez, Diego Ezequiel
BIOMATERIALS
PROTEIN BIOPOLYMERS
DSC
ACID HYDROLYSIS
SKIN TISSUE
title_short Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
title_full Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
title_fullStr Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
title_full_unstemmed Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
title_sort Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films
dc.creator.none.fl_str_mv Velazquez, Diego Ezequiel
Latorre, Maria Emilia
author Velazquez, Diego Ezequiel
author_facet Velazquez, Diego Ezequiel
Latorre, Maria Emilia
author_role author
author2 Latorre, Maria Emilia
author2_role author
dc.subject.none.fl_str_mv BIOMATERIALS
PROTEIN BIOPOLYMERS
DSC
ACID HYDROLYSIS
SKIN TISSUE
topic BIOMATERIALS
PROTEIN BIOPOLYMERS
DSC
ACID HYDROLYSIS
SKIN TISSUE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Animal tissue by-products, rich in collagen, represent a valuable source of biomaterials. Understanding their physicochemical and thermal behavior is essential for expanding their applications. In this study, pigskin gelatin was extracted through acid hydrolysis using a combination of acetic acid (AH) and either lactic, citric, or ascorbic acid (75:25, v:v, [0.5 M]), followed by thermal denaturation. We evaluated the physicochemical properties of the gelatin solutions (pH, hydroxyproline content, and extraction yield), as well as the macroscopic gel characteristics. Gelatin films were then prepared and analyzed for moisture content, color, and thermal properties. One-way ANOVA was applied to compare treatments, and Pearson’s correlation was used to assess the relationship between the solution pH and physicochemical parameters. Significant differences in the final pH of the solutions were observed among the acid mixture treatments, though the hydroxyproline content and extraction yield were not significantly affected. All gelatin solutions formed stable gels, and the resulting films exhibited similar moisture content. Thermal analysis revealed treatmentdependent variations. Specifically, a significant negative correlation (p < 0.005) was found between the gelatin solution pH and the melting temperature. These results suggest that the use of organic acid mixtures can effectively modulate gelatin properties, offering a versatile approach for tailoring biomaterials for both food and non-food applications.
Fil: Velazquez, Diego Ezequiel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto de Física de Materiales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires | Universidad Nacional del Centro de la Pcia. de Bs.as. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
Fil: Latorre, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires | Universidad Nacional del Centro de la Pcia. de Bs.as. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. Sede Olavarría del Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
description Animal tissue by-products, rich in collagen, represent a valuable source of biomaterials. Understanding their physicochemical and thermal behavior is essential for expanding their applications. In this study, pigskin gelatin was extracted through acid hydrolysis using a combination of acetic acid (AH) and either lactic, citric, or ascorbic acid (75:25, v:v, [0.5 M]), followed by thermal denaturation. We evaluated the physicochemical properties of the gelatin solutions (pH, hydroxyproline content, and extraction yield), as well as the macroscopic gel characteristics. Gelatin films were then prepared and analyzed for moisture content, color, and thermal properties. One-way ANOVA was applied to compare treatments, and Pearson’s correlation was used to assess the relationship between the solution pH and physicochemical parameters. Significant differences in the final pH of the solutions were observed among the acid mixture treatments, though the hydroxyproline content and extraction yield were not significantly affected. All gelatin solutions formed stable gels, and the resulting films exhibited similar moisture content. Thermal analysis revealed treatmentdependent variations. Specifically, a significant negative correlation (p < 0.005) was found between the gelatin solution pH and the melting temperature. These results suggest that the use of organic acid mixtures can effectively modulate gelatin properties, offering a versatile approach for tailoring biomaterials for both food and non-food applications.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272465
Velazquez, Diego Ezequiel; Latorre, Maria Emilia; Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films; Multidisciplinary Digital Publishing Institute; Physchem; 5; 3; 9-2025; 1-12
2673-7167
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272465
identifier_str_mv Velazquez, Diego Ezequiel; Latorre, Maria Emilia; Effect of Organic Acid Mixtures on the Extraction Efficiency, Physicochemical, and Thermal Properties of Pigskin Gelatin and Resulting Films; Multidisciplinary Digital Publishing Institute; Physchem; 5; 3; 9-2025; 1-12
2673-7167
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7167/5/3/38
info:eu-repo/semantics/altIdentifier/doi/10.3390/physchem5030038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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