Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

Autores
Fonseca, Maria Isabel; Fariña, Julia Inés; Sanabria, Irma Lorena; Villalba, Laura; Zapata, Pedro Dario
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many biotechnological processes pursuing sustainability search effective, inexpensive and environmentally friendly alternatives to replace conventional practices. Lignocellulolytic white rot fungi can serve for this task through their enzymatic system including laccase (Lac). One of purposes of biotechnological enzymes production processes is usually to find the best growing and secretion conditions for a particularly selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633 and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33°C) and pHs (3.5, 4.5, 5.5) under static conditions for 7, 10 and 14 days to evaluate their growing ability and Lac production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29ºC and pH 4.5 at the 10th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature and time of cultivation for the different tested fungi.
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Fil: Fariña, Julia Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;
Fil: Sanabria, Irma Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Materia
WHITE ROT FUNGI
LACCASE
CULTURE CONDITIONS
ISOENZYMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2241
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2241
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)Fonseca, Maria IsabelFariña, Julia InésSanabria, Irma LorenaVillalba, LauraZapata, Pedro DarioWHITE ROT FUNGILACCASECULTURE CONDITIONSISOENZYMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many biotechnological processes pursuing sustainability search effective, inexpensive and environmentally friendly alternatives to replace conventional practices. Lignocellulolytic white rot fungi can serve for this task through their enzymatic system including laccase (Lac). One of purposes of biotechnological enzymes production processes is usually to find the best growing and secretion conditions for a particularly selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633 and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33°C) and pHs (3.5, 4.5, 5.5) under static conditions for 7, 10 and 14 days to evaluate their growing ability and Lac production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29ºC and pH 4.5 at the 10th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature and time of cultivation for the different tested fungi.Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;Fil: Fariña, Julia Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;Fil: Sanabria, Irma Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;North Carolina State University. Department of Wood and Paper Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2241Fonseca, Maria Isabel; Fariña, Julia Inés; Sanabria, Irma Lorena; Villalba, Laura; Zapata, Pedro Dario; Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina); North Carolina State University. Department of Wood and Paper Science; Bioresources; 8; 2; 4-2013; 2855-28661930-2126enginfo:eu-repo/semantics/altIdentifier/url/http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_08_2_2855_Fonseca_Culture_Conditions_Laccase/2104info:eu-repo/semantics/altIdentifier/url/http://www.ncsu.edu/bioresources/BioRes_08/BioRes_08_2_2855_Fonseca_FSVZ_Laccase_Isoenzyme_White_Rot_Fungi_Misiones_3835.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:20Zoai:ri.conicet.gov.ar:11336/2241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:21.214CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
title Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
spellingShingle Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
Fonseca, Maria Isabel
WHITE ROT FUNGI
LACCASE
CULTURE CONDITIONS
ISOENZYMES
title_short Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
title_full Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
title_fullStr Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
title_full_unstemmed Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
title_sort Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)
dc.creator.none.fl_str_mv Fonseca, Maria Isabel
Fariña, Julia Inés
Sanabria, Irma Lorena
Villalba, Laura
Zapata, Pedro Dario
author Fonseca, Maria Isabel
author_facet Fonseca, Maria Isabel
Fariña, Julia Inés
Sanabria, Irma Lorena
Villalba, Laura
Zapata, Pedro Dario
author_role author
author2 Fariña, Julia Inés
Sanabria, Irma Lorena
Villalba, Laura
Zapata, Pedro Dario
author2_role author
author
author
author
dc.subject.none.fl_str_mv WHITE ROT FUNGI
LACCASE
CULTURE CONDITIONS
ISOENZYMES
topic WHITE ROT FUNGI
LACCASE
CULTURE CONDITIONS
ISOENZYMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many biotechnological processes pursuing sustainability search effective, inexpensive and environmentally friendly alternatives to replace conventional practices. Lignocellulolytic white rot fungi can serve for this task through their enzymatic system including laccase (Lac). One of purposes of biotechnological enzymes production processes is usually to find the best growing and secretion conditions for a particularly selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633 and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33°C) and pHs (3.5, 4.5, 5.5) under static conditions for 7, 10 and 14 days to evaluate their growing ability and Lac production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29ºC and pH 4.5 at the 10th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature and time of cultivation for the different tested fungi.
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Fil: Fariña, Julia Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;
Fil: Sanabria, Irma Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina;
description Many biotechnological processes pursuing sustainability search effective, inexpensive and environmentally friendly alternatives to replace conventional practices. Lignocellulolytic white rot fungi can serve for this task through their enzymatic system including laccase (Lac). One of purposes of biotechnological enzymes production processes is usually to find the best growing and secretion conditions for a particularly selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633 and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33°C) and pHs (3.5, 4.5, 5.5) under static conditions for 7, 10 and 14 days to evaluate their growing ability and Lac production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29ºC and pH 4.5 at the 10th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature and time of cultivation for the different tested fungi.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2241
Fonseca, Maria Isabel; Fariña, Julia Inés; Sanabria, Irma Lorena; Villalba, Laura; Zapata, Pedro Dario; Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina); North Carolina State University. Department of Wood and Paper Science; Bioresources; 8; 2; 4-2013; 2855-2866
1930-2126
url http://hdl.handle.net/11336/2241
identifier_str_mv Fonseca, Maria Isabel; Fariña, Julia Inés; Sanabria, Irma Lorena; Villalba, Laura; Zapata, Pedro Dario; Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina); North Carolina State University. Department of Wood and Paper Science; Bioresources; 8; 2; 4-2013; 2855-2866
1930-2126
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_08_2_2855_Fonseca_Culture_Conditions_Laccase/2104
info:eu-repo/semantics/altIdentifier/url/http://www.ncsu.edu/bioresources/BioRes_08/BioRes_08_2_2855_Fonseca_FSVZ_Laccase_Isoenzyme_White_Rot_Fungi_Misiones_3835.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv North Carolina State University. Department of Wood and Paper Science
publisher.none.fl_str_mv North Carolina State University. Department of Wood and Paper Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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