Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss

Autores
Rodríguez, María Emilia; Poza Viejo, Laura; Maestro Gaitán, Isaac; Schneider Teixeira, Aline; Deladino, Lorena; Ixtaina, Vanesa Yanet; Reguera, Maria
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Exposure to elevated temperatures and relative humidity expeditesthe seed aging process, finally leading to seed viability loss. In this context, certainproteins play a pivotal role in safeguarding the longevity of seeds. However, theseedproteomic response to loss viability in Salvia hispanica L., commonly knownas chia, remains incompletely understood.Methods: This work explores the application of proteomics as a potent tool foruncovering molecular responses to viability loss caused by artificial aging in twochia genotypes, WN and MN.Results: By using a quantitative label-free proteomics analysis (LC-MS/MS), 1787proteins wereidentified in chia seeds at a 95% confidence level, including storageproteins, heat shock proteins (HSPs), late embryogenesis abundant proteins (LEA),oleosins, reactive oxygen species (ROS)-related enzymes, and ribosomal proteins.A relatively low percentage of exclusive proteins were identified in viable and nonviable seeds. However, proteins exhibiting differential abundancebetween samplesindicated variations in the genotype and physiological status. Specifically, the WNgenotype showed 130 proteins with differential abundancecomparing viable andnon-viable seeds, while MN displayed changes in the abundance of 174 proteins.While both showed a significant decrease in keyproteins responsible formaintaining seed functionality, longevity, and vigor withhigh-temperature andhumidity conditions, such as LEA proteins or HSPs, ROS, and oleosins, distinctresponses between genotypes were noted, particularly in ribosomal proteins thatwere accumulated in MN and diminished in WN seeds.Discussion: Overall, the results emphasize the importance of evaluating changesin proteins of viable and non-viable seeds as they offer valuable insights into theunderlying biological mechanisms responsible for the maintenance of chia seedintegrity throughout high-temperature and humidity exposure.
Fil: Rodríguez, María Emilia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Poza Viejo, Laura. Universidad Autónoma de Madrid; España
Fil: Maestro Gaitán, Isaac. Universidad Autónoma de Madrid; España
Fil: Schneider Teixeira, Aline. YPF - Tecnología; Argentina
Fil: Deladino, Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Ixtaina, Vanesa Yanet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Reguera, Maria. Universidad Autónoma de Madrid; España
Materia
CHIA
NUTLET
SEED VIABILITY
ARTIFICIAL AGING
EMERGENT CROPS
PROTEOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247866
Acceder
id CONICETDig_1011b48975653debc904b7d7db343a2c
oai_identifier_str oai:ri.conicet.gov.ar:11336/247866
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability lossRodríguez, María EmiliaPoza Viejo, LauraMaestro Gaitán, IsaacSchneider Teixeira, AlineDeladino, LorenaIxtaina, Vanesa YanetReguera, MariaCHIANUTLETSEED VIABILITYARTIFICIAL AGINGEMERGENT CROPSPROTEOMICShttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Introduction: Exposure to elevated temperatures and relative humidity expeditesthe seed aging process, finally leading to seed viability loss. In this context, certainproteins play a pivotal role in safeguarding the longevity of seeds. However, theseedproteomic response to loss viability in Salvia hispanica L., commonly knownas chia, remains incompletely understood.Methods: This work explores the application of proteomics as a potent tool foruncovering molecular responses to viability loss caused by artificial aging in twochia genotypes, WN and MN.Results: By using a quantitative label-free proteomics analysis (LC-MS/MS), 1787proteins wereidentified in chia seeds at a 95% confidence level, including storageproteins, heat shock proteins (HSPs), late embryogenesis abundant proteins (LEA),oleosins, reactive oxygen species (ROS)-related enzymes, and ribosomal proteins.A relatively low percentage of exclusive proteins were identified in viable and nonviable seeds. However, proteins exhibiting differential abundancebetween samplesindicated variations in the genotype and physiological status. Specifically, the WNgenotype showed 130 proteins with differential abundancecomparing viable andnon-viable seeds, while MN displayed changes in the abundance of 174 proteins.While both showed a significant decrease in keyproteins responsible formaintaining seed functionality, longevity, and vigor withhigh-temperature andhumidity conditions, such as LEA proteins or HSPs, ROS, and oleosins, distinctresponses between genotypes were noted, particularly in ribosomal proteins thatwere accumulated in MN and diminished in WN seeds.Discussion: Overall, the results emphasize the importance of evaluating changesin proteins of viable and non-viable seeds as they offer valuable insights into theunderlying biological mechanisms responsible for the maintenance of chia seedintegrity throughout high-temperature and humidity exposure.Fil: Rodríguez, María Emilia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Poza Viejo, Laura. Universidad Autónoma de Madrid; EspañaFil: Maestro Gaitán, Isaac. Universidad Autónoma de Madrid; EspañaFil: Schneider Teixeira, Aline. YPF - Tecnología; ArgentinaFil: Deladino, Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Ixtaina, Vanesa Yanet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Reguera, Maria. Universidad Autónoma de Madrid; EspañaFrontiers2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247866Rodríguez, María Emilia; Poza Viejo, Laura; Maestro Gaitán, Isaac; Schneider Teixeira, Aline; Deladino, Lorena; et al.; Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss; Frontiers; Frontiers in Plant Science; 15; 8-2024; 1-141664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2024.1441234/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2024.1441234info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:01Zoai:ri.conicet.gov.ar:11336/247866instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:01.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
title Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
spellingShingle Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
Rodríguez, María Emilia
CHIA
NUTLET
SEED VIABILITY
ARTIFICIAL AGING
EMERGENT CROPS
PROTEOMICS
title_short Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
title_full Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
title_fullStr Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
title_full_unstemmed Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
title_sort Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss
dc.creator.none.fl_str_mv Rodríguez, María Emilia
Poza Viejo, Laura
Maestro Gaitán, Isaac
Schneider Teixeira, Aline
Deladino, Lorena
Ixtaina, Vanesa Yanet
Reguera, Maria
author Rodríguez, María Emilia
author_facet Rodríguez, María Emilia
Poza Viejo, Laura
Maestro Gaitán, Isaac
Schneider Teixeira, Aline
Deladino, Lorena
Ixtaina, Vanesa Yanet
Reguera, Maria
author_role author
author2 Poza Viejo, Laura
Maestro Gaitán, Isaac
Schneider Teixeira, Aline
Deladino, Lorena
Ixtaina, Vanesa Yanet
Reguera, Maria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHIA
NUTLET
SEED VIABILITY
ARTIFICIAL AGING
EMERGENT CROPS
PROTEOMICS
topic CHIA
NUTLET
SEED VIABILITY
ARTIFICIAL AGING
EMERGENT CROPS
PROTEOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Introduction: Exposure to elevated temperatures and relative humidity expeditesthe seed aging process, finally leading to seed viability loss. In this context, certainproteins play a pivotal role in safeguarding the longevity of seeds. However, theseedproteomic response to loss viability in Salvia hispanica L., commonly knownas chia, remains incompletely understood.Methods: This work explores the application of proteomics as a potent tool foruncovering molecular responses to viability loss caused by artificial aging in twochia genotypes, WN and MN.Results: By using a quantitative label-free proteomics analysis (LC-MS/MS), 1787proteins wereidentified in chia seeds at a 95% confidence level, including storageproteins, heat shock proteins (HSPs), late embryogenesis abundant proteins (LEA),oleosins, reactive oxygen species (ROS)-related enzymes, and ribosomal proteins.A relatively low percentage of exclusive proteins were identified in viable and nonviable seeds. However, proteins exhibiting differential abundancebetween samplesindicated variations in the genotype and physiological status. Specifically, the WNgenotype showed 130 proteins with differential abundancecomparing viable andnon-viable seeds, while MN displayed changes in the abundance of 174 proteins.While both showed a significant decrease in keyproteins responsible formaintaining seed functionality, longevity, and vigor withhigh-temperature andhumidity conditions, such as LEA proteins or HSPs, ROS, and oleosins, distinctresponses between genotypes were noted, particularly in ribosomal proteins thatwere accumulated in MN and diminished in WN seeds.Discussion: Overall, the results emphasize the importance of evaluating changesin proteins of viable and non-viable seeds as they offer valuable insights into theunderlying biological mechanisms responsible for the maintenance of chia seedintegrity throughout high-temperature and humidity exposure.
Fil: Rodríguez, María Emilia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Poza Viejo, Laura. Universidad Autónoma de Madrid; España
Fil: Maestro Gaitán, Isaac. Universidad Autónoma de Madrid; España
Fil: Schneider Teixeira, Aline. YPF - Tecnología; Argentina
Fil: Deladino, Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Ixtaina, Vanesa Yanet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Reguera, Maria. Universidad Autónoma de Madrid; España
description Introduction: Exposure to elevated temperatures and relative humidity expeditesthe seed aging process, finally leading to seed viability loss. In this context, certainproteins play a pivotal role in safeguarding the longevity of seeds. However, theseedproteomic response to loss viability in Salvia hispanica L., commonly knownas chia, remains incompletely understood.Methods: This work explores the application of proteomics as a potent tool foruncovering molecular responses to viability loss caused by artificial aging in twochia genotypes, WN and MN.Results: By using a quantitative label-free proteomics analysis (LC-MS/MS), 1787proteins wereidentified in chia seeds at a 95% confidence level, including storageproteins, heat shock proteins (HSPs), late embryogenesis abundant proteins (LEA),oleosins, reactive oxygen species (ROS)-related enzymes, and ribosomal proteins.A relatively low percentage of exclusive proteins were identified in viable and nonviable seeds. However, proteins exhibiting differential abundancebetween samplesindicated variations in the genotype and physiological status. Specifically, the WNgenotype showed 130 proteins with differential abundancecomparing viable andnon-viable seeds, while MN displayed changes in the abundance of 174 proteins.While both showed a significant decrease in keyproteins responsible formaintaining seed functionality, longevity, and vigor withhigh-temperature andhumidity conditions, such as LEA proteins or HSPs, ROS, and oleosins, distinctresponses between genotypes were noted, particularly in ribosomal proteins thatwere accumulated in MN and diminished in WN seeds.Discussion: Overall, the results emphasize the importance of evaluating changesin proteins of viable and non-viable seeds as they offer valuable insights into theunderlying biological mechanisms responsible for the maintenance of chia seedintegrity throughout high-temperature and humidity exposure.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247866
Rodríguez, María Emilia; Poza Viejo, Laura; Maestro Gaitán, Isaac; Schneider Teixeira, Aline; Deladino, Lorena; et al.; Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss; Frontiers; Frontiers in Plant Science; 15; 8-2024; 1-14
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247866
identifier_str_mv Rodríguez, María Emilia; Poza Viejo, Laura; Maestro Gaitán, Isaac; Schneider Teixeira, Aline; Deladino, Lorena; et al.; Shotgun proteomics profiling of chia seeds (Salvia hispanica L.) reveals genotypic differential responses to viability loss; Frontiers; Frontiers in Plant Science; 15; 8-2024; 1-14
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2024.1441234/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2024.1441234
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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