Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay

Autores
Pan, D.; Mionetto, A.; Calero, N.; Reynoso, Maria Marta; Torres, Adriana Mabel; Bettucci, L.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding its population genetics. Therefore, isolates of F. graminearum s.s. from the major wheat-growing region of Uruguay were analyzed by amplified fragment length polymorphism assays, PCR genotyping, and chemical analysis of trichothecene production. Of the 102 isolates identified as having the 15-ADON genotype via PCR genotyping, all were DON producers, but only 41 strains were also 15-ADON producers, as determined by chemical analysis. The populations were genotypically diverse but genetically similar, with significant genetic exchange occurring between them. Analysis of molecular variance indicated that most of the genetic variability resulted from differences between isolates within populations. Multilocus linkage disequilibrium analysis suggested that the isolates had a panmictic population genetic structure and that there is significant recombination occurs in F. graminearum s.s. In conclusion, tour findings provide the first detailed description of the genetic structure and trichothecene production of populations of F. graminearum s.s. from Uruguay, and expands our understanding of the agroecology of F. graminearum and of the correlation between genotypes and trichothecene chemotypes.
Fil: Pan, D.. Universidad de la República; Uruguay
Fil: Mionetto, A.. Universidad de la República; Uruguay
Fil: Calero, N.. Universidad de la República; Uruguay
Fil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Torres, Adriana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Bettucci, L.. Universidad de la República; Uruguay
Materia
FUSARIUM GRAMINEARUM
FUSARIUM HEAD BLIGHT
GENETIC STRUCTURE
TRICHOTHECENE
WHEAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180060
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180060
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in UruguayPan, D.Mionetto, A.Calero, N.Reynoso, Maria MartaTorres, Adriana MabelBettucci, L.FUSARIUM GRAMINEARUMFUSARIUM HEAD BLIGHTGENETIC STRUCTURETRICHOTHECENEWHEAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding its population genetics. Therefore, isolates of F. graminearum s.s. from the major wheat-growing region of Uruguay were analyzed by amplified fragment length polymorphism assays, PCR genotyping, and chemical analysis of trichothecene production. Of the 102 isolates identified as having the 15-ADON genotype via PCR genotyping, all were DON producers, but only 41 strains were also 15-ADON producers, as determined by chemical analysis. The populations were genotypically diverse but genetically similar, with significant genetic exchange occurring between them. Analysis of molecular variance indicated that most of the genetic variability resulted from differences between isolates within populations. Multilocus linkage disequilibrium analysis suggested that the isolates had a panmictic population genetic structure and that there is significant recombination occurs in F. graminearum s.s. In conclusion, tour findings provide the first detailed description of the genetic structure and trichothecene production of populations of F. graminearum s.s. from Uruguay, and expands our understanding of the agroecology of F. graminearum and of the correlation between genotypes and trichothecene chemotypes.Fil: Pan, D.. Universidad de la República; UruguayFil: Mionetto, A.. Universidad de la República; UruguayFil: Calero, N.. Universidad de la República; UruguayFil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Torres, Adriana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Bettucci, L.. Universidad de la República; UruguayFundacao de Pesquisas Científicas de Riberao Preto2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180060Pan, D.; Mionetto, A.; Calero, N.; Reynoso, Maria Marta; Torres, Adriana Mabel; et al.; Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 15; 1; 3-2016; 1-111676-5680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.geneticsmr.com/articles/6113info:eu-repo/semantics/altIdentifier/doi/10.4238/gmr.15017270info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:25Zoai:ri.conicet.gov.ar:11336/180060instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:26.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
title Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
spellingShingle Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
Pan, D.
FUSARIUM GRAMINEARUM
FUSARIUM HEAD BLIGHT
GENETIC STRUCTURE
TRICHOTHECENE
WHEAT
title_short Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
title_full Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
title_fullStr Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
title_full_unstemmed Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
title_sort Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
dc.creator.none.fl_str_mv Pan, D.
Mionetto, A.
Calero, N.
Reynoso, Maria Marta
Torres, Adriana Mabel
Bettucci, L.
author Pan, D.
author_facet Pan, D.
Mionetto, A.
Calero, N.
Reynoso, Maria Marta
Torres, Adriana Mabel
Bettucci, L.
author_role author
author2 Mionetto, A.
Calero, N.
Reynoso, Maria Marta
Torres, Adriana Mabel
Bettucci, L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FUSARIUM GRAMINEARUM
FUSARIUM HEAD BLIGHT
GENETIC STRUCTURE
TRICHOTHECENE
WHEAT
topic FUSARIUM GRAMINEARUM
FUSARIUM HEAD BLIGHT
GENETIC STRUCTURE
TRICHOTHECENE
WHEAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding its population genetics. Therefore, isolates of F. graminearum s.s. from the major wheat-growing region of Uruguay were analyzed by amplified fragment length polymorphism assays, PCR genotyping, and chemical analysis of trichothecene production. Of the 102 isolates identified as having the 15-ADON genotype via PCR genotyping, all were DON producers, but only 41 strains were also 15-ADON producers, as determined by chemical analysis. The populations were genotypically diverse but genetically similar, with significant genetic exchange occurring between them. Analysis of molecular variance indicated that most of the genetic variability resulted from differences between isolates within populations. Multilocus linkage disequilibrium analysis suggested that the isolates had a panmictic population genetic structure and that there is significant recombination occurs in F. graminearum s.s. In conclusion, tour findings provide the first detailed description of the genetic structure and trichothecene production of populations of F. graminearum s.s. from Uruguay, and expands our understanding of the agroecology of F. graminearum and of the correlation between genotypes and trichothecene chemotypes.
Fil: Pan, D.. Universidad de la República; Uruguay
Fil: Mionetto, A.. Universidad de la República; Uruguay
Fil: Calero, N.. Universidad de la República; Uruguay
Fil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Torres, Adriana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Bettucci, L.. Universidad de la República; Uruguay
description Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding its population genetics. Therefore, isolates of F. graminearum s.s. from the major wheat-growing region of Uruguay were analyzed by amplified fragment length polymorphism assays, PCR genotyping, and chemical analysis of trichothecene production. Of the 102 isolates identified as having the 15-ADON genotype via PCR genotyping, all were DON producers, but only 41 strains were also 15-ADON producers, as determined by chemical analysis. The populations were genotypically diverse but genetically similar, with significant genetic exchange occurring between them. Analysis of molecular variance indicated that most of the genetic variability resulted from differences between isolates within populations. Multilocus linkage disequilibrium analysis suggested that the isolates had a panmictic population genetic structure and that there is significant recombination occurs in F. graminearum s.s. In conclusion, tour findings provide the first detailed description of the genetic structure and trichothecene production of populations of F. graminearum s.s. from Uruguay, and expands our understanding of the agroecology of F. graminearum and of the correlation between genotypes and trichothecene chemotypes.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180060
Pan, D.; Mionetto, A.; Calero, N.; Reynoso, Maria Marta; Torres, Adriana Mabel; et al.; Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 15; 1; 3-2016; 1-11
1676-5680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180060
identifier_str_mv Pan, D.; Mionetto, A.; Calero, N.; Reynoso, Maria Marta; Torres, Adriana Mabel; et al.; Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 15; 1; 3-2016; 1-11
1676-5680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.geneticsmr.com/articles/6113
info:eu-repo/semantics/altIdentifier/doi/10.4238/gmr.15017270
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundacao de Pesquisas Científicas de Riberao Preto
publisher.none.fl_str_mv Fundacao de Pesquisas Científicas de Riberao Preto
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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