Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings

Autores
Gatica Sosa, Claudia; Brzek, Pawel; Chediack, Juan Gabriel; Cid, Fabricio Damian; Karasov, William H.; Caviedes Vidal, Enrique Juan Raul
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many species show diet-induced flexibility of activity of intestinal enzymes; however, molecular and genetic mechanisms responsible for such modulation are less known, particularly in altricial birds. The goal of our study was to test whether a diet-induced increase of activity of intestinal maltase and sucrase in house sparrow nestlings is matched with an increase in maltase-glucoamylase (MG) and sucrase-isomaltase (SI) complex mRNAs, respectively. Both enzyme activities were significantly higher in mid intestine of nestlings fed a medium starch (MS) diet compared to those fed a starch-free (SF) diet. In contrast to the similar pattern of dietary induction for both enzyme activities, diet MS elevated significantly only the level of MG mRNA, but not SI mRNA. The coordinated increase in activity of maltase and in MG mRNA is consistent with the hypothesis that dietary induction of this enzyme is under transcriptional control. In contrast, the lack of such coordination for changes in activity of sucrase and SI mRNA suggests that up-regulation of this enzyme may be achieved by post-translational factor(s). We conclude that genetic mechanisms responsible for diet-induced flexibility of digestive enzymes in birds may differ from that observed in mammals.
Fil: Gatica Sosa, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Brzek, Pawel. University of Bialystok; Polonia
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Karasov, William H.. University of Wisconsin-Madison; Estados Unidos
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. University Of Wisconsin; Estados Unidos
Materia
Enzyme Activity
Gene Expression
Small Intestine
House Sparrow
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8286
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8286
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlingsGatica Sosa, ClaudiaBrzek, PawelChediack, Juan GabrielCid, Fabricio DamianKarasov, William H.Caviedes Vidal, Enrique Juan RaulEnzyme ActivityGene ExpressionSmall IntestineHouse Sparrowhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many species show diet-induced flexibility of activity of intestinal enzymes; however, molecular and genetic mechanisms responsible for such modulation are less known, particularly in altricial birds. The goal of our study was to test whether a diet-induced increase of activity of intestinal maltase and sucrase in house sparrow nestlings is matched with an increase in maltase-glucoamylase (MG) and sucrase-isomaltase (SI) complex mRNAs, respectively. Both enzyme activities were significantly higher in mid intestine of nestlings fed a medium starch (MS) diet compared to those fed a starch-free (SF) diet. In contrast to the similar pattern of dietary induction for both enzyme activities, diet MS elevated significantly only the level of MG mRNA, but not SI mRNA. The coordinated increase in activity of maltase and in MG mRNA is consistent with the hypothesis that dietary induction of this enzyme is under transcriptional control. In contrast, the lack of such coordination for changes in activity of sucrase and SI mRNA suggests that up-regulation of this enzyme may be achieved by post-translational factor(s). We conclude that genetic mechanisms responsible for diet-induced flexibility of digestive enzymes in birds may differ from that observed in mammals.Fil: Gatica Sosa, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Brzek, Pawel. University of Bialystok; PoloniaFil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Karasov, William H.. University of Wisconsin-Madison; Estados UnidosFil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. University Of Wisconsin; Estados UnidosWiley2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8286Gatica Sosa, Claudia; Brzek, Pawel; Chediack, Juan Gabriel; Cid, Fabricio Damian; Karasov, William H.; et al.; Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings; Wiley; Journal Of Animal Physiology And Animal Nutrition; 100; 2; 6-2015; 236–2420931-2439enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jpn.12354/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/jpn.12354info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:09Zoai:ri.conicet.gov.ar:11336/8286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:09.71CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
title Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
spellingShingle Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
Gatica Sosa, Claudia
Enzyme Activity
Gene Expression
Small Intestine
House Sparrow
title_short Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
title_full Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
title_fullStr Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
title_full_unstemmed Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
title_sort Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings
dc.creator.none.fl_str_mv Gatica Sosa, Claudia
Brzek, Pawel
Chediack, Juan Gabriel
Cid, Fabricio Damian
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
author Gatica Sosa, Claudia
author_facet Gatica Sosa, Claudia
Brzek, Pawel
Chediack, Juan Gabriel
Cid, Fabricio Damian
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
author_role author
author2 Brzek, Pawel
Chediack, Juan Gabriel
Cid, Fabricio Damian
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enzyme Activity
Gene Expression
Small Intestine
House Sparrow
topic Enzyme Activity
Gene Expression
Small Intestine
House Sparrow
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many species show diet-induced flexibility of activity of intestinal enzymes; however, molecular and genetic mechanisms responsible for such modulation are less known, particularly in altricial birds. The goal of our study was to test whether a diet-induced increase of activity of intestinal maltase and sucrase in house sparrow nestlings is matched with an increase in maltase-glucoamylase (MG) and sucrase-isomaltase (SI) complex mRNAs, respectively. Both enzyme activities were significantly higher in mid intestine of nestlings fed a medium starch (MS) diet compared to those fed a starch-free (SF) diet. In contrast to the similar pattern of dietary induction for both enzyme activities, diet MS elevated significantly only the level of MG mRNA, but not SI mRNA. The coordinated increase in activity of maltase and in MG mRNA is consistent with the hypothesis that dietary induction of this enzyme is under transcriptional control. In contrast, the lack of such coordination for changes in activity of sucrase and SI mRNA suggests that up-regulation of this enzyme may be achieved by post-translational factor(s). We conclude that genetic mechanisms responsible for diet-induced flexibility of digestive enzymes in birds may differ from that observed in mammals.
Fil: Gatica Sosa, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Brzek, Pawel. University of Bialystok; Polonia
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Karasov, William H.. University of Wisconsin-Madison; Estados Unidos
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. University Of Wisconsin; Estados Unidos
description Many species show diet-induced flexibility of activity of intestinal enzymes; however, molecular and genetic mechanisms responsible for such modulation are less known, particularly in altricial birds. The goal of our study was to test whether a diet-induced increase of activity of intestinal maltase and sucrase in house sparrow nestlings is matched with an increase in maltase-glucoamylase (MG) and sucrase-isomaltase (SI) complex mRNAs, respectively. Both enzyme activities were significantly higher in mid intestine of nestlings fed a medium starch (MS) diet compared to those fed a starch-free (SF) diet. In contrast to the similar pattern of dietary induction for both enzyme activities, diet MS elevated significantly only the level of MG mRNA, but not SI mRNA. The coordinated increase in activity of maltase and in MG mRNA is consistent with the hypothesis that dietary induction of this enzyme is under transcriptional control. In contrast, the lack of such coordination for changes in activity of sucrase and SI mRNA suggests that up-regulation of this enzyme may be achieved by post-translational factor(s). We conclude that genetic mechanisms responsible for diet-induced flexibility of digestive enzymes in birds may differ from that observed in mammals.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8286
Gatica Sosa, Claudia; Brzek, Pawel; Chediack, Juan Gabriel; Cid, Fabricio Damian; Karasov, William H.; et al.; Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings; Wiley; Journal Of Animal Physiology And Animal Nutrition; 100; 2; 6-2015; 236–242
0931-2439
url http://hdl.handle.net/11336/8286
identifier_str_mv Gatica Sosa, Claudia; Brzek, Pawel; Chediack, Juan Gabriel; Cid, Fabricio Damian; Karasov, William H.; et al.; Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings; Wiley; Journal Of Animal Physiology And Animal Nutrition; 100; 2; 6-2015; 236–242
0931-2439
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jpn.12354/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/jpn.12354
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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