Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.

Autores
Mompeón, Ana; Lázaro Franco, Macarena; Bueno Betí, Carlos; Pérez Cremades, Daniel; Vidal Gómez, Xavier; Monsalve, Elena; Gironacci, Mariela Mercedes; Hermenegildo, Carlos; Novella, Susana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intracellular renin-angiotensin system (RAS) can operate independently of the circulating RAS. Estrogens provide protective effects by modulating the RAS. Our aim was to investigate the effect of estradiol (E2) on angiotensin converting enzymes (ACE) 1 and ACE2 expression and activities in human endothelial cells (HUVEC), and the role of estrogen receptors (ER). The results confirmed the presence of active intracellular RAS in HUVEC. Physiological concentrations of E2 induced a concentration-dependent increase of ACE1 and ACE2 mRNA expression and ACE1, but not ACE2, protein levels. ACE1 and ACE2 enzymatic activities were also induced with E2. These effects were mediated through ERα activation, since ER antagonists ICI 182780 and MPP completely abolished the effect of E2. Moreover, the ERα agonist PPT mirrored the E2 effects on ACE1 and ACE2 protein expression and activity. Exposure of endothelial cells to E2 significantly increased Ang-(1–7) production. In conclusion, E2 increases Ang-(1–7) production, through ERα, involving increased ACE1 and ACE2 mRNA expression and activity and ACE1 protein levels.
Fil: Mompeón, Ana. Universidad de Valencia; España
Fil: Lázaro Franco, Macarena. Universidad de Valencia; España
Fil: Bueno Betí, Carlos. Universidad de Valencia; España
Fil: Pérez Cremades, Daniel. Universidad de Valencia; España
Fil: Vidal Gómez, Xavier. Universidad de Valencia; España
Fil: Monsalve, Elena. Universidad de Valencia; España
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Hermenegildo, Carlos. Universidad de Valencia; España
Fil: Novella, Susana. Universidad de Valencia; España
Materia
Estrogen
Endothelial Cell
Angiotensin Converting Enzymes
Renin Angiotensin System
Estrogen Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18259
Acceder
id CONICETDig_0ec9f062a6b846b1006e0271f1f36fae
oai_identifier_str oai:ri.conicet.gov.ar:11336/18259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.Mompeón, AnaLázaro Franco, MacarenaBueno Betí, CarlosPérez Cremades, DanielVidal Gómez, XavierMonsalve, ElenaGironacci, Mariela MercedesHermenegildo, CarlosNovella, SusanaEstrogenEndothelial CellAngiotensin Converting EnzymesRenin Angiotensin SystemEstrogen Receptorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Intracellular renin-angiotensin system (RAS) can operate independently of the circulating RAS. Estrogens provide protective effects by modulating the RAS. Our aim was to investigate the effect of estradiol (E2) on angiotensin converting enzymes (ACE) 1 and ACE2 expression and activities in human endothelial cells (HUVEC), and the role of estrogen receptors (ER). The results confirmed the presence of active intracellular RAS in HUVEC. Physiological concentrations of E2 induced a concentration-dependent increase of ACE1 and ACE2 mRNA expression and ACE1, but not ACE2, protein levels. ACE1 and ACE2 enzymatic activities were also induced with E2. These effects were mediated through ERα activation, since ER antagonists ICI 182780 and MPP completely abolished the effect of E2. Moreover, the ERα agonist PPT mirrored the E2 effects on ACE1 and ACE2 protein expression and activity. Exposure of endothelial cells to E2 significantly increased Ang-(1–7) production. In conclusion, E2 increases Ang-(1–7) production, through ERα, involving increased ACE1 and ACE2 mRNA expression and activity and ACE1 protein levels.Fil: Mompeón, Ana. Universidad de Valencia; EspañaFil: Lázaro Franco, Macarena. Universidad de Valencia; EspañaFil: Bueno Betí, Carlos. Universidad de Valencia; EspañaFil: Pérez Cremades, Daniel. Universidad de Valencia; EspañaFil: Vidal Gómez, Xavier. Universidad de Valencia; EspañaFil: Monsalve, Elena. Universidad de Valencia; EspañaFil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Hermenegildo, Carlos. Universidad de Valencia; EspañaFil: Novella, Susana. Universidad de Valencia; EspañaElsevier Ireland2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18259Mompeón, Ana; Lázaro Franco, Macarena; Bueno Betí, Carlos; Pérez Cremades, Daniel; Vidal Gómez, Xavier; et al.; Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.; Elsevier Ireland; Molecular And Cellular Endocrinology; 422; 2-2016; 1-80303-7207CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720715301295info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2015.11.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:35Zoai:ri.conicet.gov.ar:11336/18259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:35.339CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
title Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
spellingShingle Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
Mompeón, Ana
Estrogen
Endothelial Cell
Angiotensin Converting Enzymes
Renin Angiotensin System
Estrogen Receptor
title_short Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
title_full Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
title_fullStr Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
title_full_unstemmed Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
title_sort Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.
dc.creator.none.fl_str_mv Mompeón, Ana
Lázaro Franco, Macarena
Bueno Betí, Carlos
Pérez Cremades, Daniel
Vidal Gómez, Xavier
Monsalve, Elena
Gironacci, Mariela Mercedes
Hermenegildo, Carlos
Novella, Susana
author Mompeón, Ana
author_facet Mompeón, Ana
Lázaro Franco, Macarena
Bueno Betí, Carlos
Pérez Cremades, Daniel
Vidal Gómez, Xavier
Monsalve, Elena
Gironacci, Mariela Mercedes
Hermenegildo, Carlos
Novella, Susana
author_role author
author2 Lázaro Franco, Macarena
Bueno Betí, Carlos
Pérez Cremades, Daniel
Vidal Gómez, Xavier
Monsalve, Elena
Gironacci, Mariela Mercedes
Hermenegildo, Carlos
Novella, Susana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrogen
Endothelial Cell
Angiotensin Converting Enzymes
Renin Angiotensin System
Estrogen Receptor
topic Estrogen
Endothelial Cell
Angiotensin Converting Enzymes
Renin Angiotensin System
Estrogen Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Intracellular renin-angiotensin system (RAS) can operate independently of the circulating RAS. Estrogens provide protective effects by modulating the RAS. Our aim was to investigate the effect of estradiol (E2) on angiotensin converting enzymes (ACE) 1 and ACE2 expression and activities in human endothelial cells (HUVEC), and the role of estrogen receptors (ER). The results confirmed the presence of active intracellular RAS in HUVEC. Physiological concentrations of E2 induced a concentration-dependent increase of ACE1 and ACE2 mRNA expression and ACE1, but not ACE2, protein levels. ACE1 and ACE2 enzymatic activities were also induced with E2. These effects were mediated through ERα activation, since ER antagonists ICI 182780 and MPP completely abolished the effect of E2. Moreover, the ERα agonist PPT mirrored the E2 effects on ACE1 and ACE2 protein expression and activity. Exposure of endothelial cells to E2 significantly increased Ang-(1–7) production. In conclusion, E2 increases Ang-(1–7) production, through ERα, involving increased ACE1 and ACE2 mRNA expression and activity and ACE1 protein levels.
Fil: Mompeón, Ana. Universidad de Valencia; España
Fil: Lázaro Franco, Macarena. Universidad de Valencia; España
Fil: Bueno Betí, Carlos. Universidad de Valencia; España
Fil: Pérez Cremades, Daniel. Universidad de Valencia; España
Fil: Vidal Gómez, Xavier. Universidad de Valencia; España
Fil: Monsalve, Elena. Universidad de Valencia; España
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Hermenegildo, Carlos. Universidad de Valencia; España
Fil: Novella, Susana. Universidad de Valencia; España
description Intracellular renin-angiotensin system (RAS) can operate independently of the circulating RAS. Estrogens provide protective effects by modulating the RAS. Our aim was to investigate the effect of estradiol (E2) on angiotensin converting enzymes (ACE) 1 and ACE2 expression and activities in human endothelial cells (HUVEC), and the role of estrogen receptors (ER). The results confirmed the presence of active intracellular RAS in HUVEC. Physiological concentrations of E2 induced a concentration-dependent increase of ACE1 and ACE2 mRNA expression and ACE1, but not ACE2, protein levels. ACE1 and ACE2 enzymatic activities were also induced with E2. These effects were mediated through ERα activation, since ER antagonists ICI 182780 and MPP completely abolished the effect of E2. Moreover, the ERα agonist PPT mirrored the E2 effects on ACE1 and ACE2 protein expression and activity. Exposure of endothelial cells to E2 significantly increased Ang-(1–7) production. In conclusion, E2 increases Ang-(1–7) production, through ERα, involving increased ACE1 and ACE2 mRNA expression and activity and ACE1 protein levels.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18259
Mompeón, Ana; Lázaro Franco, Macarena; Bueno Betí, Carlos; Pérez Cremades, Daniel; Vidal Gómez, Xavier; et al.; Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.; Elsevier Ireland; Molecular And Cellular Endocrinology; 422; 2-2016; 1-8
0303-7207
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18259
identifier_str_mv Mompeón, Ana; Lázaro Franco, Macarena; Bueno Betí, Carlos; Pérez Cremades, Daniel; Vidal Gómez, Xavier; et al.; Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production.; Elsevier Ireland; Molecular And Cellular Endocrinology; 422; 2-2016; 1-8
0303-7207
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720715301295
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2015.11.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613148334620672
score 13.069144

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