GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock

Autores
Oliveiro, Karina Andrea; Crepy, Maria Andrea; Martin Tryon, Ellen L.; Milich, Raechel; Harmer, Stacey L.; Putterill, Jo; Yanovsky, Marcelo Javier; Casal, Jorge José
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of gi have defects in the hypocotyl-growth and cotyledon-unfolding responses to hourly pulses of FR, a treatment perceived by phytochrome A (phyA). This phenotype is rescued by overexpression of GI. The very- low-fluence response of seed germination was also reduced in gi . Since the circadian clock modulates many light responses, we investigated whether these gi phenotypes were due to alterations in the circadian system or light signaling per se. In exper- iments where FR pulses were given to dark-incubated seeds or seedlings at different times of the day, gi showed reduced seed germination, cotyledon unfolding, and activity of a luciferase reporter fused to the promoter of a chlorophyll a/b -binding protein gene; however, rhythmic sensitivity was normal in these plants. We conclude that while GI does not affect the high- irradiance responses of phyA, it does affect phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functions
Fil: Oliveiro, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Crepy, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Martin Tryon, Ellen L.. University of California; Estados Unidos
Fil: Milich, Raechel. The University Of Auckland; Nueva Zelanda
Fil: Harmer, Stacey L.. University of California; Estados Unidos
Fil: Putterill, Jo. The University Of Auckland; Nueva Zelanda
Fil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Flowering
Photomorphogenesis
Arabidopsis Thaliana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31050
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31050
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clockOliveiro, Karina AndreaCrepy, Maria AndreaMartin Tryon, Ellen L.Milich, RaechelHarmer, Stacey L.Putterill, JoYanovsky, Marcelo JavierCasal, Jorge JoséFloweringPhotomorphogenesisArabidopsis Thalianahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of gi have defects in the hypocotyl-growth and cotyledon-unfolding responses to hourly pulses of FR, a treatment perceived by phytochrome A (phyA). This phenotype is rescued by overexpression of GI. The very- low-fluence response of seed germination was also reduced in gi . Since the circadian clock modulates many light responses, we investigated whether these gi phenotypes were due to alterations in the circadian system or light signaling per se. In exper- iments where FR pulses were given to dark-incubated seeds or seedlings at different times of the day, gi showed reduced seed germination, cotyledon unfolding, and activity of a luciferase reporter fused to the promoter of a chlorophyll a/b -binding protein gene; however, rhythmic sensitivity was normal in these plants. We conclude that while GI does not affect the high- irradiance responses of phyA, it does affect phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functionsFil: Oliveiro, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Crepy, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Martin Tryon, Ellen L.. University of California; Estados UnidosFil: Milich, Raechel. The University Of Auckland; Nueva ZelandaFil: Harmer, Stacey L.. University of California; Estados UnidosFil: Putterill, Jo. The University Of Auckland; Nueva ZelandaFil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaAmerican Society of Plant Biologist2007-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31050Casal, Jorge José; Yanovsky, Marcelo Javier; Putterill, Jo; Harmer, Stacey L.; Oliveiro, Karina Andrea; Milich, Raechel; et al.; GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock; American Society of Plant Biologist; Plant Physiology; 144; 1; 3-2007; 495-5020032-08891532-2548CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/144/1/495.longinfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.107.097048info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:04Zoai:ri.conicet.gov.ar:11336/31050instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:04.9CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
title GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
spellingShingle GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
Oliveiro, Karina Andrea
Flowering
Photomorphogenesis
Arabidopsis Thaliana
title_short GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
title_full GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
title_fullStr GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
title_full_unstemmed GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
title_sort GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
dc.creator.none.fl_str_mv Oliveiro, Karina Andrea
Crepy, Maria Andrea
Martin Tryon, Ellen L.
Milich, Raechel
Harmer, Stacey L.
Putterill, Jo
Yanovsky, Marcelo Javier
Casal, Jorge José
author Oliveiro, Karina Andrea
author_facet Oliveiro, Karina Andrea
Crepy, Maria Andrea
Martin Tryon, Ellen L.
Milich, Raechel
Harmer, Stacey L.
Putterill, Jo
Yanovsky, Marcelo Javier
Casal, Jorge José
author_role author
author2 Crepy, Maria Andrea
Martin Tryon, Ellen L.
Milich, Raechel
Harmer, Stacey L.
Putterill, Jo
Yanovsky, Marcelo Javier
Casal, Jorge José
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Flowering
Photomorphogenesis
Arabidopsis Thaliana
topic Flowering
Photomorphogenesis
Arabidopsis Thaliana
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of gi have defects in the hypocotyl-growth and cotyledon-unfolding responses to hourly pulses of FR, a treatment perceived by phytochrome A (phyA). This phenotype is rescued by overexpression of GI. The very- low-fluence response of seed germination was also reduced in gi . Since the circadian clock modulates many light responses, we investigated whether these gi phenotypes were due to alterations in the circadian system or light signaling per se. In exper- iments where FR pulses were given to dark-incubated seeds or seedlings at different times of the day, gi showed reduced seed germination, cotyledon unfolding, and activity of a luciferase reporter fused to the promoter of a chlorophyll a/b -binding protein gene; however, rhythmic sensitivity was normal in these plants. We conclude that while GI does not affect the high- irradiance responses of phyA, it does affect phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functions
Fil: Oliveiro, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Crepy, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Martin Tryon, Ellen L.. University of California; Estados Unidos
Fil: Milich, Raechel. The University Of Auckland; Nueva Zelanda
Fil: Harmer, Stacey L.. University of California; Estados Unidos
Fil: Putterill, Jo. The University Of Auckland; Nueva Zelanda
Fil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of gi have defects in the hypocotyl-growth and cotyledon-unfolding responses to hourly pulses of FR, a treatment perceived by phytochrome A (phyA). This phenotype is rescued by overexpression of GI. The very- low-fluence response of seed germination was also reduced in gi . Since the circadian clock modulates many light responses, we investigated whether these gi phenotypes were due to alterations in the circadian system or light signaling per se. In exper- iments where FR pulses were given to dark-incubated seeds or seedlings at different times of the day, gi showed reduced seed germination, cotyledon unfolding, and activity of a luciferase reporter fused to the promoter of a chlorophyll a/b -binding protein gene; however, rhythmic sensitivity was normal in these plants. We conclude that while GI does not affect the high- irradiance responses of phyA, it does affect phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functions
publishDate 2007
dc.date.none.fl_str_mv 2007-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31050
Casal, Jorge José; Yanovsky, Marcelo Javier; Putterill, Jo; Harmer, Stacey L.; Oliveiro, Karina Andrea; Milich, Raechel; et al.; GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock; American Society of Plant Biologist; Plant Physiology; 144; 1; 3-2007; 495-502
0032-0889
1532-2548
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31050
identifier_str_mv Casal, Jorge José; Yanovsky, Marcelo Javier; Putterill, Jo; Harmer, Stacey L.; Oliveiro, Karina Andrea; Milich, Raechel; et al.; GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock; American Society of Plant Biologist; Plant Physiology; 144; 1; 3-2007; 495-502
0032-0889
1532-2548
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/144/1/495.long
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.107.097048
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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