A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibiti...

Autores: Pérez Giménez, Julieta; Iturralde, Esteban Tomás; Torres Tejerizo, Gonzalo Arturo; Quelas, Juan Ignacio; Krol, Elizaveta; Borassi, Cecilia; Becker, Anke; Estevez, Jose Manuel; Lodeiro, Anibal
Año de publicación: 2021
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: When subjected to nutritional stress, bacteria modify their amino acid metabolism and cell division activities by means of the stringent response, which is controlled by the Rsh protein in alphaproteobacteria. An important group of alphaproteobacteria are the rhizobia, which fix atmospheric N2 in symbiosis with legume plants. Although nutritional stress is common for rhizobia while infecting legume roots, the stringent response was scarcely studied in this group of soil bacteria. In this report, we obtained a mutant in the rsh gene of Bradyrhizobium diazoefficiens, the N2-fixing symbiont of soybean. This mutant was defective for type-3-secretion system induction, plant-defense suppression at early root infection, and competition for nodulation. Furthermore, the mutant produced smaller nodules, although with normal morphology, which lead to lower plant biomass production. Soybean genes GmRIC1 and GmRIC2, involved in autoregulation of nodulation, were upregulated in plants inoculated with the mutant in N-free condition. In addition, when plants were inoculated in the presence of 10 mM NH4NO3, the mutant produced nodules containing bacteroids, and GmRIC1 and GmRIC2 were downregulated. The rsh mutant released more auxin to the culture supernatant than the wild type, which might in part explain its symbiotic behavior in the presence of combined-N. These results indicate that B. diazoefficiens stringent response integrates into the plant defense suppression and regulation of nodulation circuits in soybean, perhaps mediated by the type-3-secretion system.IMPORTANCE The symbiotic N2 fixation carried out between prokaryotic rhizobia and legume plants performs a substantial contribution to the N-cycle in the biosphere. This symbiotic association is initiated when rhizobia infect and penetrate the root hairs, which is followed by the growth and development of root nodules within which the infective rhizobia are established and protected. Thus, the nodule environment allows the expression and function of the enzyme complex that catalyzes N2 fixation. However, during early infection the rhizobia find a harsh environment while penetrating the root hairs. To cope with this nuisance, the rhizobia mount a stress response known as stringent response. In turn, the plant regulates nodulation in response to the presence of alternative sources of combined-N in the surrounding medium. Control of these processes is crucial for a successful symbiosis, and here we show how the rhizobial stringent response may modulate plant defense suppression and the networks of regulation of nodulation.
Fil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Iturralde, Esteban Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Krol, Elizaveta. Philipps-Universität Marburg; Alemania
Fil: Borassi, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Becker, Anke. Philipps-Universität Marburg; Alemania
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Andrés Bello; Chile
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia: BRADYRHIZOBIUM DIAZOEFFICIENS
STRINGENT RESPONSE
PLANT DEFENSE
SYMBIOSIS
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/155931

Acceder

id	CONICETDig_0dcc2ed5abad7c00dde057952215777b
oai_identifier_str	oai:ri.conicet.gov.ar:11336/155931
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulationPérez Giménez, JulietaIturralde, Esteban TomásTorres Tejerizo, Gonzalo ArturoQuelas, Juan IgnacioKrol, ElizavetaBorassi, CeciliaBecker, AnkeEstevez, Jose ManuelLodeiro, AnibalBRADYRHIZOBIUM DIAZOEFFICIENSSTRINGENT RESPONSEPLANT DEFENSESYMBIOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1When subjected to nutritional stress, bacteria modify their amino acid metabolism and cell division activities by means of the stringent response, which is controlled by the Rsh protein in alphaproteobacteria. An important group of alphaproteobacteria are the rhizobia, which fix atmospheric N2 in symbiosis with legume plants. Although nutritional stress is common for rhizobia while infecting legume roots, the stringent response was scarcely studied in this group of soil bacteria. In this report, we obtained a mutant in the rsh gene of Bradyrhizobium diazoefficiens, the N2-fixing symbiont of soybean. This mutant was defective for type-3-secretion system induction, plant-defense suppression at early root infection, and competition for nodulation. Furthermore, the mutant produced smaller nodules, although with normal morphology, which lead to lower plant biomass production. Soybean genes GmRIC1 and GmRIC2, involved in autoregulation of nodulation, were upregulated in plants inoculated with the mutant in N-free condition. In addition, when plants were inoculated in the presence of 10 mM NH4NO3, the mutant produced nodules containing bacteroids, and GmRIC1 and GmRIC2 were downregulated. The rsh mutant released more auxin to the culture supernatant than the wild type, which might in part explain its symbiotic behavior in the presence of combined-N. These results indicate that B. diazoefficiens stringent response integrates into the plant defense suppression and regulation of nodulation circuits in soybean, perhaps mediated by the type-3-secretion system.IMPORTANCE The symbiotic N2 fixation carried out between prokaryotic rhizobia and legume plants performs a substantial contribution to the N-cycle in the biosphere. This symbiotic association is initiated when rhizobia infect and penetrate the root hairs, which is followed by the growth and development of root nodules within which the infective rhizobia are established and protected. Thus, the nodule environment allows the expression and function of the enzyme complex that catalyzes N2 fixation. However, during early infection the rhizobia find a harsh environment while penetrating the root hairs. To cope with this nuisance, the rhizobia mount a stress response known as stringent response. In turn, the plant regulates nodulation in response to the presence of alternative sources of combined-N in the surrounding medium. Control of these processes is crucial for a successful symbiosis, and here we show how the rhizobial stringent response may modulate plant defense suppression and the networks of regulation of nodulation.Fil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Iturralde, Esteban Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Krol, Elizaveta. Philipps-Universität Marburg; AlemaniaFil: Borassi, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Becker, Anke. Philipps-Universität Marburg; AlemaniaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Andrés Bello; ChileFil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAmerican Society for Microbiology2021-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155931Pérez Giménez, Julieta; Iturralde, Esteban Tomás; Torres Tejerizo, Gonzalo Arturo; Quelas, Juan Ignacio; Krol, Elizaveta; et al.; A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation; American Society for Microbiology; Applied and Environmental Microbiology; 87; 9; 13-4-2021; 1-140099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/lookup/doi/10.1128/AEM.02989-20info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02989-20info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:51Zoai:ri.conicet.gov.ar:11336/155931instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:51.812CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
title	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
spellingShingle	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation Pérez Giménez, Julieta BRADYRHIZOBIUM DIAZOEFFICIENS STRINGENT RESPONSE PLANT DEFENSE SYMBIOSIS
title_short	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
title_full	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
title_fullStr	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
title_full_unstemmed	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
title_sort	A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation
dc.creator.none.fl_str_mv	Pérez Giménez, Julieta Iturralde, Esteban Tomás Torres Tejerizo, Gonzalo Arturo Quelas, Juan Ignacio Krol, Elizaveta Borassi, Cecilia Becker, Anke Estevez, Jose Manuel Lodeiro, Anibal
author	Pérez Giménez, Julieta
author_facet	Pérez Giménez, Julieta Iturralde, Esteban Tomás Torres Tejerizo, Gonzalo Arturo Quelas, Juan Ignacio Krol, Elizaveta Borassi, Cecilia Becker, Anke Estevez, Jose Manuel Lodeiro, Anibal
author_role	author
author2	Iturralde, Esteban Tomás Torres Tejerizo, Gonzalo Arturo Quelas, Juan Ignacio Krol, Elizaveta Borassi, Cecilia Becker, Anke Estevez, Jose Manuel Lodeiro, Anibal
author2_role	author author author author author author author author
dc.subject.none.fl_str_mv	BRADYRHIZOBIUM DIAZOEFFICIENS STRINGENT RESPONSE PLANT DEFENSE SYMBIOSIS
topic	BRADYRHIZOBIUM DIAZOEFFICIENS STRINGENT RESPONSE PLANT DEFENSE SYMBIOSIS
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	When subjected to nutritional stress, bacteria modify their amino acid metabolism and cell division activities by means of the stringent response, which is controlled by the Rsh protein in alphaproteobacteria. An important group of alphaproteobacteria are the rhizobia, which fix atmospheric N2 in symbiosis with legume plants. Although nutritional stress is common for rhizobia while infecting legume roots, the stringent response was scarcely studied in this group of soil bacteria. In this report, we obtained a mutant in the rsh gene of Bradyrhizobium diazoefficiens, the N2-fixing symbiont of soybean. This mutant was defective for type-3-secretion system induction, plant-defense suppression at early root infection, and competition for nodulation. Furthermore, the mutant produced smaller nodules, although with normal morphology, which lead to lower plant biomass production. Soybean genes GmRIC1 and GmRIC2, involved in autoregulation of nodulation, were upregulated in plants inoculated with the mutant in N-free condition. In addition, when plants were inoculated in the presence of 10 mM NH4NO3, the mutant produced nodules containing bacteroids, and GmRIC1 and GmRIC2 were downregulated. The rsh mutant released more auxin to the culture supernatant than the wild type, which might in part explain its symbiotic behavior in the presence of combined-N. These results indicate that B. diazoefficiens stringent response integrates into the plant defense suppression and regulation of nodulation circuits in soybean, perhaps mediated by the type-3-secretion system.IMPORTANCE The symbiotic N2 fixation carried out between prokaryotic rhizobia and legume plants performs a substantial contribution to the N-cycle in the biosphere. This symbiotic association is initiated when rhizobia infect and penetrate the root hairs, which is followed by the growth and development of root nodules within which the infective rhizobia are established and protected. Thus, the nodule environment allows the expression and function of the enzyme complex that catalyzes N2 fixation. However, during early infection the rhizobia find a harsh environment while penetrating the root hairs. To cope with this nuisance, the rhizobia mount a stress response known as stringent response. In turn, the plant regulates nodulation in response to the presence of alternative sources of combined-N in the surrounding medium. Control of these processes is crucial for a successful symbiosis, and here we show how the rhizobial stringent response may modulate plant defense suppression and the networks of regulation of nodulation. Fil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Iturralde, Esteban Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Krol, Elizaveta. Philipps-Universität Marburg; Alemania Fil: Borassi, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Becker, Anke. Philipps-Universität Marburg; Alemania Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Andrés Bello; Chile Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description	When subjected to nutritional stress, bacteria modify their amino acid metabolism and cell division activities by means of the stringent response, which is controlled by the Rsh protein in alphaproteobacteria. An important group of alphaproteobacteria are the rhizobia, which fix atmospheric N2 in symbiosis with legume plants. Although nutritional stress is common for rhizobia while infecting legume roots, the stringent response was scarcely studied in this group of soil bacteria. In this report, we obtained a mutant in the rsh gene of Bradyrhizobium diazoefficiens, the N2-fixing symbiont of soybean. This mutant was defective for type-3-secretion system induction, plant-defense suppression at early root infection, and competition for nodulation. Furthermore, the mutant produced smaller nodules, although with normal morphology, which lead to lower plant biomass production. Soybean genes GmRIC1 and GmRIC2, involved in autoregulation of nodulation, were upregulated in plants inoculated with the mutant in N-free condition. In addition, when plants were inoculated in the presence of 10 mM NH4NO3, the mutant produced nodules containing bacteroids, and GmRIC1 and GmRIC2 were downregulated. The rsh mutant released more auxin to the culture supernatant than the wild type, which might in part explain its symbiotic behavior in the presence of combined-N. These results indicate that B. diazoefficiens stringent response integrates into the plant defense suppression and regulation of nodulation circuits in soybean, perhaps mediated by the type-3-secretion system.IMPORTANCE The symbiotic N2 fixation carried out between prokaryotic rhizobia and legume plants performs a substantial contribution to the N-cycle in the biosphere. This symbiotic association is initiated when rhizobia infect and penetrate the root hairs, which is followed by the growth and development of root nodules within which the infective rhizobia are established and protected. Thus, the nodule environment allows the expression and function of the enzyme complex that catalyzes N2 fixation. However, during early infection the rhizobia find a harsh environment while penetrating the root hairs. To cope with this nuisance, the rhizobia mount a stress response known as stringent response. In turn, the plant regulates nodulation in response to the presence of alternative sources of combined-N in the surrounding medium. Control of these processes is crucial for a successful symbiosis, and here we show how the rhizobial stringent response may modulate plant defense suppression and the networks of regulation of nodulation.
publishDate	2021
dc.date.none.fl_str_mv	2021-04-13
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/155931 Pérez Giménez, Julieta; Iturralde, Esteban Tomás; Torres Tejerizo, Gonzalo Arturo; Quelas, Juan Ignacio; Krol, Elizaveta; et al.; A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation; American Society for Microbiology; Applied and Environmental Microbiology; 87; 9; 13-4-2021; 1-14 0099-2240 CONICET Digital CONICET
url	http://hdl.handle.net/11336/155931
identifier_str_mv	Pérez Giménez, Julieta; Iturralde, Esteban Tomás; Torres Tejerizo, Gonzalo Arturo; Quelas, Juan Ignacio; Krol, Elizaveta; et al.; A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibition of nodulation; American Society for Microbiology; Applied and Environmental Microbiology; 87; 9; 13-4-2021; 1-14 0099-2240 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/lookup/doi/10.1128/AEM.02989-20 info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02989-20
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	American Society for Microbiology
publisher.none.fl_str_mv	American Society for Microbiology
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1842269663418908672
score	13.13397

A stringent response-defective Bradyrhizobium diazoefficiens does not activate the type-3-secretion system, elicits early plant defense, and circumvents NH 4 NO 3 -induced inhibiti...

Publicaciones similares