Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata

Autores
Campoy Díaz, Alejandra Daniela; Malanga, Gabriela Fabiana; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The freshwater gastropod Pomacea canaliculata fulfills the ideal conditions of a bioindicator species since its digestive gland bioconcentrates elements toxic for human and ecosystems health. The aim of this work was to study the balance between production of free radicals and antioxidant defenses, and the generation of oxidative damage in the digestive gland of this mollusk after exposure (96 h) to three elements with differential affinities for functional biological groups: mercury (5.5 μg/L of Hg+2 as HgCl2), arsenic [500 μg/L of (AsO4)−3 as Na3AsO47H2O], or uranium [700 μg/L of (UO2)+2 as UO2(CH2COOH)2]. Bioconcentration factors of Hg, As, and U were 25, 23, and 53, respectively. Snails exhibited a sustained increase of reactive species (RS), and protein and lipid damage. Lipid radicals increased between 72 and 96 h, respectively, in snails exposed to U and Hg while this parameter changed early (24 h) in As- exposed snails. Snails showed protein damage, reaching maximum values at different endpoints. This redox disbalance was partially compensated by non-enzymatic antioxidant defenses α-tocopherol (α-T), β-carotene (β-C), uric acid, metallothionein (MTs). Snails consumed α-T and β-C in an element-dependent manner. The digestive gland consumed rapidly uric acid and this molecule was not recovered at 96 h. Digestive gland showed a significant increase in MTs after elemental exposure at different endpoints. The enzymatic antioxidant defenses, represented by the catalase and glutathione-S-transferase activities, seems to be not necessary for the early stages of the oxidative process by metals. This work is the first attempt to elucidate cellular mechanisms involved in the tolerance of this gastropod to non-essential elements. The bioconcentration factors and changes in the oxidative status and damage confirm that this species can be used as a bioindicator species of metal pollution in freshwater bodies.
Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes. Escuela de Cs. de la Salud; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Malanga, Gabriela Fabiana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Giraud Billoud, Maximiliano German. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes. Escuela de Cs. de la Salud; Argentina
Fil: Vega, Israel Aníbal. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
ANTIOXIDANT DEFENSE
APPLE SNAIL
ARSENIC
MERCURY
OXIDATIVE DAMAGE
OXIDATIVE STRESS
URANIUM
WATER POLLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228328
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228328
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network_name_str CONICET Digital (CONICET)
spelling Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculataCampoy Díaz, Alejandra DanielaMalanga, Gabriela FabianaGiraud Billoud, Maximiliano GermanVega, Israel AníbalANTIOXIDANT DEFENSEAPPLE SNAILARSENICMERCURYOXIDATIVE DAMAGEOXIDATIVE STRESSURANIUMWATER POLLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The freshwater gastropod Pomacea canaliculata fulfills the ideal conditions of a bioindicator species since its digestive gland bioconcentrates elements toxic for human and ecosystems health. The aim of this work was to study the balance between production of free radicals and antioxidant defenses, and the generation of oxidative damage in the digestive gland of this mollusk after exposure (96 h) to three elements with differential affinities for functional biological groups: mercury (5.5 μg/L of Hg+2 as HgCl2), arsenic [500 μg/L of (AsO4)−3 as Na3AsO47H2O], or uranium [700 μg/L of (UO2)+2 as UO2(CH2COOH)2]. Bioconcentration factors of Hg, As, and U were 25, 23, and 53, respectively. Snails exhibited a sustained increase of reactive species (RS), and protein and lipid damage. Lipid radicals increased between 72 and 96 h, respectively, in snails exposed to U and Hg while this parameter changed early (24 h) in As- exposed snails. Snails showed protein damage, reaching maximum values at different endpoints. This redox disbalance was partially compensated by non-enzymatic antioxidant defenses α-tocopherol (α-T), β-carotene (β-C), uric acid, metallothionein (MTs). Snails consumed α-T and β-C in an element-dependent manner. The digestive gland consumed rapidly uric acid and this molecule was not recovered at 96 h. Digestive gland showed a significant increase in MTs after elemental exposure at different endpoints. The enzymatic antioxidant defenses, represented by the catalase and glutathione-S-transferase activities, seems to be not necessary for the early stages of the oxidative process by metals. This work is the first attempt to elucidate cellular mechanisms involved in the tolerance of this gastropod to non-essential elements. The bioconcentration factors and changes in the oxidative status and damage confirm that this species can be used as a bioindicator species of metal pollution in freshwater bodies.Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes. Escuela de Cs. de la Salud; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; ArgentinaFil: Malanga, Gabriela Fabiana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Giraud Billoud, Maximiliano German. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes. Escuela de Cs. de la Salud; ArgentinaFil: Vega, Israel Aníbal. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFrontiers Media2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228328Campoy Díaz, Alejandra Daniela; Malanga, Gabriela Fabiana; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata; Frontiers Media; Frontiers in Physiology; 14; 3-2023; 1-101664-042XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fphys.2023.1123977/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fphys.2023.1123977info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:45:10Zoai:ri.conicet.gov.ar:11336/228328instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:45:11.214CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
title Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
spellingShingle Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
Campoy Díaz, Alejandra Daniela
ANTIOXIDANT DEFENSE
APPLE SNAIL
ARSENIC
MERCURY
OXIDATIVE DAMAGE
OXIDATIVE STRESS
URANIUM
WATER POLLUTION
title_short Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
title_full Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
title_fullStr Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
title_full_unstemmed Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
title_sort Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata
dc.creator.none.fl_str_mv Campoy Díaz, Alejandra Daniela
Malanga, Gabriela Fabiana
Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
author Campoy Díaz, Alejandra Daniela
author_facet Campoy Díaz, Alejandra Daniela
Malanga, Gabriela Fabiana
Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
author_role author
author2 Malanga, Gabriela Fabiana
Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT DEFENSE
APPLE SNAIL
ARSENIC
MERCURY
OXIDATIVE DAMAGE
OXIDATIVE STRESS
URANIUM
WATER POLLUTION
topic ANTIOXIDANT DEFENSE
APPLE SNAIL
ARSENIC
MERCURY
OXIDATIVE DAMAGE
OXIDATIVE STRESS
URANIUM
WATER POLLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The freshwater gastropod Pomacea canaliculata fulfills the ideal conditions of a bioindicator species since its digestive gland bioconcentrates elements toxic for human and ecosystems health. The aim of this work was to study the balance between production of free radicals and antioxidant defenses, and the generation of oxidative damage in the digestive gland of this mollusk after exposure (96 h) to three elements with differential affinities for functional biological groups: mercury (5.5 μg/L of Hg+2 as HgCl2), arsenic [500 μg/L of (AsO4)−3 as Na3AsO47H2O], or uranium [700 μg/L of (UO2)+2 as UO2(CH2COOH)2]. Bioconcentration factors of Hg, As, and U were 25, 23, and 53, respectively. Snails exhibited a sustained increase of reactive species (RS), and protein and lipid damage. Lipid radicals increased between 72 and 96 h, respectively, in snails exposed to U and Hg while this parameter changed early (24 h) in As- exposed snails. Snails showed protein damage, reaching maximum values at different endpoints. This redox disbalance was partially compensated by non-enzymatic antioxidant defenses α-tocopherol (α-T), β-carotene (β-C), uric acid, metallothionein (MTs). Snails consumed α-T and β-C in an element-dependent manner. The digestive gland consumed rapidly uric acid and this molecule was not recovered at 96 h. Digestive gland showed a significant increase in MTs after elemental exposure at different endpoints. The enzymatic antioxidant defenses, represented by the catalase and glutathione-S-transferase activities, seems to be not necessary for the early stages of the oxidative process by metals. This work is the first attempt to elucidate cellular mechanisms involved in the tolerance of this gastropod to non-essential elements. The bioconcentration factors and changes in the oxidative status and damage confirm that this species can be used as a bioindicator species of metal pollution in freshwater bodies.
Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes. Escuela de Cs. de la Salud; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Malanga, Gabriela Fabiana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Giraud Billoud, Maximiliano German. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes. Escuela de Cs. de la Salud; Argentina
Fil: Vega, Israel Aníbal. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description The freshwater gastropod Pomacea canaliculata fulfills the ideal conditions of a bioindicator species since its digestive gland bioconcentrates elements toxic for human and ecosystems health. The aim of this work was to study the balance between production of free radicals and antioxidant defenses, and the generation of oxidative damage in the digestive gland of this mollusk after exposure (96 h) to three elements with differential affinities for functional biological groups: mercury (5.5 μg/L of Hg+2 as HgCl2), arsenic [500 μg/L of (AsO4)−3 as Na3AsO47H2O], or uranium [700 μg/L of (UO2)+2 as UO2(CH2COOH)2]. Bioconcentration factors of Hg, As, and U were 25, 23, and 53, respectively. Snails exhibited a sustained increase of reactive species (RS), and protein and lipid damage. Lipid radicals increased between 72 and 96 h, respectively, in snails exposed to U and Hg while this parameter changed early (24 h) in As- exposed snails. Snails showed protein damage, reaching maximum values at different endpoints. This redox disbalance was partially compensated by non-enzymatic antioxidant defenses α-tocopherol (α-T), β-carotene (β-C), uric acid, metallothionein (MTs). Snails consumed α-T and β-C in an element-dependent manner. The digestive gland consumed rapidly uric acid and this molecule was not recovered at 96 h. Digestive gland showed a significant increase in MTs after elemental exposure at different endpoints. The enzymatic antioxidant defenses, represented by the catalase and glutathione-S-transferase activities, seems to be not necessary for the early stages of the oxidative process by metals. This work is the first attempt to elucidate cellular mechanisms involved in the tolerance of this gastropod to non-essential elements. The bioconcentration factors and changes in the oxidative status and damage confirm that this species can be used as a bioindicator species of metal pollution in freshwater bodies.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228328
Campoy Díaz, Alejandra Daniela; Malanga, Gabriela Fabiana; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata; Frontiers Media; Frontiers in Physiology; 14; 3-2023; 1-10
1664-042X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228328
identifier_str_mv Campoy Díaz, Alejandra Daniela; Malanga, Gabriela Fabiana; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Changes in the oxidative status and damage by non-essential elements in the digestive gland of the gastropod Pomacea canaliculata; Frontiers Media; Frontiers in Physiology; 14; 3-2023; 1-10
1664-042X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fphys.2023.1123977/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fphys.2023.1123977
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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