Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size

Autores
Zhang, Yong Jiang; Bucci, Sandra Janet; Arias, Nadia Soledad; Scholz, Fabian Gustavo; Hao, Guang You; Cao, Kun Fang; Goldstein, Guillermo Hernan
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Freezing resistance through avoidance or tolerance of extracellular ice nucleation is important for plant survival in habitats with frequent subzero temperatures. However, the role of cell walls in leaf freezing resistance and the coordination between leaf and stem physiological processes under subzero temperatures are not well understood. We studied leaf and stem responses to freezing temperatures, leaf and stem supercooling, leaf bulk elastic modulus and stem xylem vessel size of six Patagonian shrub species from two sites (plateau and low elevation sites) with different elevation and minimum temperatures. Ice seeding was initiated in the stem and quickly spread to leaves, but two species from the plateau site had barriers against rapid spread of ice. Shrubs with xylem vessels smaller in diameter had greater stem supercooling capacity, i.e., ice nucleated at lower subzero temperatures. Only one species with the lowest ice nucleation temperature among all species studied exhibited freezing avoidance by substantial supercooling, while the rest were able to tolerate extracellular freezing from −11.3 to −20 °C. Leaves of species with more rigid cell walls (higher bulk elastic modulus) could survive freezing to lower subzero temperatures, suggesting that rigid cell walls potentially reduce the degree of physical injury to cell membranes during the extracellular freezing and/or thaw processes. In conclusion, our results reveal the temporal–spatial ice spreading pattern (from stem to leaves) in Patagonian shrubs, and indicate the role of xylem vessel size in determining supercooling capacity and the role of cell wall elasticity in determining leaf tolerance of extracellular ice formation.
Fil: Zhang, Yong Jiang. Chinese Academy of Sciences; República de China. Harvard University; Estados Unidos
Fil: Bucci, Sandra Janet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; Argentina
Fil: Arias, Nadia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; Argentina
Fil: Scholz, Fabian Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; Argentina
Fil: Hao, Guang You. Chinese Academy of Sciences; República de China
Fil: Cao, Kun Fang. Guangxi University; China
Fil: Goldstein, Guillermo Hernan. University of Miami; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bulk Elastic Modulus
Patagonian Steppe
Leaf Lethal Temperature
Supercooling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46053
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel sizeZhang, Yong JiangBucci, Sandra JanetArias, Nadia SoledadScholz, Fabian GustavoHao, Guang YouCao, Kun FangGoldstein, Guillermo HernanBulk Elastic ModulusPatagonian SteppeLeaf Lethal TemperatureSupercoolinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Freezing resistance through avoidance or tolerance of extracellular ice nucleation is important for plant survival in habitats with frequent subzero temperatures. However, the role of cell walls in leaf freezing resistance and the coordination between leaf and stem physiological processes under subzero temperatures are not well understood. We studied leaf and stem responses to freezing temperatures, leaf and stem supercooling, leaf bulk elastic modulus and stem xylem vessel size of six Patagonian shrub species from two sites (plateau and low elevation sites) with different elevation and minimum temperatures. Ice seeding was initiated in the stem and quickly spread to leaves, but two species from the plateau site had barriers against rapid spread of ice. Shrubs with xylem vessels smaller in diameter had greater stem supercooling capacity, i.e., ice nucleated at lower subzero temperatures. Only one species with the lowest ice nucleation temperature among all species studied exhibited freezing avoidance by substantial supercooling, while the rest were able to tolerate extracellular freezing from −11.3 to −20 °C. Leaves of species with more rigid cell walls (higher bulk elastic modulus) could survive freezing to lower subzero temperatures, suggesting that rigid cell walls potentially reduce the degree of physical injury to cell membranes during the extracellular freezing and/or thaw processes. In conclusion, our results reveal the temporal–spatial ice spreading pattern (from stem to leaves) in Patagonian shrubs, and indicate the role of xylem vessel size in determining supercooling capacity and the role of cell wall elasticity in determining leaf tolerance of extracellular ice formation.Fil: Zhang, Yong Jiang. Chinese Academy of Sciences; República de China. Harvard University; Estados UnidosFil: Bucci, Sandra Janet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; ArgentinaFil: Arias, Nadia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; ArgentinaFil: Scholz, Fabian Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; ArgentinaFil: Hao, Guang You. Chinese Academy of Sciences; República de ChinaFil: Cao, Kun Fang. Guangxi University; ChinaFil: Goldstein, Guillermo Hernan. University of Miami; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaOxford University Press2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46053Zhang, Yong Jiang; Bucci, Sandra Janet; Arias, Nadia Soledad; Scholz, Fabian Gustavo; Hao, Guang You; et al.; Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size; Oxford University Press; Tree Physiology; 36; 8; 3-2016; 1007-10180829-318X1758-4469CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/treephys/tpw036info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/treephys/article/36/8/1007/2515579info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:57:56Zoai:ri.conicet.gov.ar:11336/46053instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:57:57.21CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
title Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
spellingShingle Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
Zhang, Yong Jiang
Bulk Elastic Modulus
Patagonian Steppe
Leaf Lethal Temperature
Supercooling
title_short Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
title_full Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
title_fullStr Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
title_full_unstemmed Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
title_sort Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size
dc.creator.none.fl_str_mv Zhang, Yong Jiang
Bucci, Sandra Janet
Arias, Nadia Soledad
Scholz, Fabian Gustavo
Hao, Guang You
Cao, Kun Fang
Goldstein, Guillermo Hernan
author Zhang, Yong Jiang
author_facet Zhang, Yong Jiang
Bucci, Sandra Janet
Arias, Nadia Soledad
Scholz, Fabian Gustavo
Hao, Guang You
Cao, Kun Fang
Goldstein, Guillermo Hernan
author_role author
author2 Bucci, Sandra Janet
Arias, Nadia Soledad
Scholz, Fabian Gustavo
Hao, Guang You
Cao, Kun Fang
Goldstein, Guillermo Hernan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bulk Elastic Modulus
Patagonian Steppe
Leaf Lethal Temperature
Supercooling
topic Bulk Elastic Modulus
Patagonian Steppe
Leaf Lethal Temperature
Supercooling
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Freezing resistance through avoidance or tolerance of extracellular ice nucleation is important for plant survival in habitats with frequent subzero temperatures. However, the role of cell walls in leaf freezing resistance and the coordination between leaf and stem physiological processes under subzero temperatures are not well understood. We studied leaf and stem responses to freezing temperatures, leaf and stem supercooling, leaf bulk elastic modulus and stem xylem vessel size of six Patagonian shrub species from two sites (plateau and low elevation sites) with different elevation and minimum temperatures. Ice seeding was initiated in the stem and quickly spread to leaves, but two species from the plateau site had barriers against rapid spread of ice. Shrubs with xylem vessels smaller in diameter had greater stem supercooling capacity, i.e., ice nucleated at lower subzero temperatures. Only one species with the lowest ice nucleation temperature among all species studied exhibited freezing avoidance by substantial supercooling, while the rest were able to tolerate extracellular freezing from −11.3 to −20 °C. Leaves of species with more rigid cell walls (higher bulk elastic modulus) could survive freezing to lower subzero temperatures, suggesting that rigid cell walls potentially reduce the degree of physical injury to cell membranes during the extracellular freezing and/or thaw processes. In conclusion, our results reveal the temporal–spatial ice spreading pattern (from stem to leaves) in Patagonian shrubs, and indicate the role of xylem vessel size in determining supercooling capacity and the role of cell wall elasticity in determining leaf tolerance of extracellular ice formation.
Fil: Zhang, Yong Jiang. Chinese Academy of Sciences; República de China. Harvard University; Estados Unidos
Fil: Bucci, Sandra Janet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; Argentina
Fil: Arias, Nadia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; Argentina
Fil: Scholz, Fabian Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia ; Argentina
Fil: Hao, Guang You. Chinese Academy of Sciences; República de China
Fil: Cao, Kun Fang. Guangxi University; China
Fil: Goldstein, Guillermo Hernan. University of Miami; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Freezing resistance through avoidance or tolerance of extracellular ice nucleation is important for plant survival in habitats with frequent subzero temperatures. However, the role of cell walls in leaf freezing resistance and the coordination between leaf and stem physiological processes under subzero temperatures are not well understood. We studied leaf and stem responses to freezing temperatures, leaf and stem supercooling, leaf bulk elastic modulus and stem xylem vessel size of six Patagonian shrub species from two sites (plateau and low elevation sites) with different elevation and minimum temperatures. Ice seeding was initiated in the stem and quickly spread to leaves, but two species from the plateau site had barriers against rapid spread of ice. Shrubs with xylem vessels smaller in diameter had greater stem supercooling capacity, i.e., ice nucleated at lower subzero temperatures. Only one species with the lowest ice nucleation temperature among all species studied exhibited freezing avoidance by substantial supercooling, while the rest were able to tolerate extracellular freezing from −11.3 to −20 °C. Leaves of species with more rigid cell walls (higher bulk elastic modulus) could survive freezing to lower subzero temperatures, suggesting that rigid cell walls potentially reduce the degree of physical injury to cell membranes during the extracellular freezing and/or thaw processes. In conclusion, our results reveal the temporal–spatial ice spreading pattern (from stem to leaves) in Patagonian shrubs, and indicate the role of xylem vessel size in determining supercooling capacity and the role of cell wall elasticity in determining leaf tolerance of extracellular ice formation.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46053
Zhang, Yong Jiang; Bucci, Sandra Janet; Arias, Nadia Soledad; Scholz, Fabian Gustavo; Hao, Guang You; et al.; Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size; Oxford University Press; Tree Physiology; 36; 8; 3-2016; 1007-1018
0829-318X
1758-4469
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46053
identifier_str_mv Zhang, Yong Jiang; Bucci, Sandra Janet; Arias, Nadia Soledad; Scholz, Fabian Gustavo; Hao, Guang You; et al.; Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size; Oxford University Press; Tree Physiology; 36; 8; 3-2016; 1007-1018
0829-318X
1758-4469
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/treephys/tpw036
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/treephys/article/36/8/1007/2515579
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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