Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts

Autores
Mechoud, Monica Adriana; Mateos, Melina Valeria; Font, Graciela Maria; Villena, Julio Cesar; Salvador, Gabriela Alejandra; Rodriguez, Ana Virginia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate TNF-α production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced production of TNF-α. In addition, Lr-S treatment activated ERK and p38 MAPK pathways in PBMC. Lipid rafts participation in the reduced production of TNF-α by PBMC induced by Lr-S was verified by lipid rafts disruption with methyl-β-cyclodextrin and the reduction of the Src-tyrosine kinase Lck localization in rafts. Moreover, PBMC pre-treatment with Lck inhibitors blocked the effect of Lr-S on TNF-α production suggesting that activation and mobilization of Lck from lipid rafts would be involved in the modulatory effect of L. reuteri CRL1098. A secreted peptide of 5785 Da would be responsible of the modulatory effect of CRL1098 strain. This study demonstrated for the first time the lipid rafts participation in a response induced by a beneficial bacterium. Also, these results open new possibilities for investigating the molecular mechanisms involved in the interaction of probiotic bacterial extracellular compounds with immune cells.
Fil: Mechoud, Monica Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rodriguez, Ana Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Lactobacillus Reuteri Crl 1098
Lipid Rafts
Pbmc
Tnf-Α
Secreted Peptide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25073
Acceder
id CONICETDig_0c81fd0f4fb8ecdba3f2969f5be40c83
oai_identifier_str oai:ri.conicet.gov.ar:11336/25073
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid raftsMechoud, Monica AdrianaMateos, Melina ValeriaFont, Graciela MariaVillena, Julio CesarSalvador, Gabriela AlejandraRodriguez, Ana VirginiaLactobacillus Reuteri Crl 1098Lipid RaftsPbmcTnf-ΑSecreted Peptidehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate TNF-α production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced production of TNF-α. In addition, Lr-S treatment activated ERK and p38 MAPK pathways in PBMC. Lipid rafts participation in the reduced production of TNF-α by PBMC induced by Lr-S was verified by lipid rafts disruption with methyl-β-cyclodextrin and the reduction of the Src-tyrosine kinase Lck localization in rafts. Moreover, PBMC pre-treatment with Lck inhibitors blocked the effect of Lr-S on TNF-α production suggesting that activation and mobilization of Lck from lipid rafts would be involved in the modulatory effect of L. reuteri CRL1098. A secreted peptide of 5785 Da would be responsible of the modulatory effect of CRL1098 strain. This study demonstrated for the first time the lipid rafts participation in a response induced by a beneficial bacterium. Also, these results open new possibilities for investigating the molecular mechanisms involved in the interaction of probiotic bacterial extracellular compounds with immune cells.Fil: Mechoud, Monica Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rodriguez, Ana Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaElsevier2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25073Mechoud, Monica Adriana; Mateos, Melina Valeria; Font, Graciela Maria; Villena, Julio Cesar; Salvador, Gabriela Alejandra; et al.; Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts; Elsevier; International Immunopharmacology; 14; 4; 10-2012; 446-4531567-57691878-1705CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.intimp.2012.08.020info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567576912002627info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:20Zoai:ri.conicet.gov.ar:11336/25073instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:20.739CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
title Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
spellingShingle Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
Mechoud, Monica Adriana
Lactobacillus Reuteri Crl 1098
Lipid Rafts
Pbmc
Tnf-Α
Secreted Peptide
title_short Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
title_full Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
title_fullStr Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
title_full_unstemmed Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
title_sort Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
dc.creator.none.fl_str_mv Mechoud, Monica Adriana
Mateos, Melina Valeria
Font, Graciela Maria
Villena, Julio Cesar
Salvador, Gabriela Alejandra
Rodriguez, Ana Virginia
author Mechoud, Monica Adriana
author_facet Mechoud, Monica Adriana
Mateos, Melina Valeria
Font, Graciela Maria
Villena, Julio Cesar
Salvador, Gabriela Alejandra
Rodriguez, Ana Virginia
author_role author
author2 Mateos, Melina Valeria
Font, Graciela Maria
Villena, Julio Cesar
Salvador, Gabriela Alejandra
Rodriguez, Ana Virginia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Reuteri Crl 1098
Lipid Rafts
Pbmc
Tnf-Α
Secreted Peptide
topic Lactobacillus Reuteri Crl 1098
Lipid Rafts
Pbmc
Tnf-Α
Secreted Peptide
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate TNF-α production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced production of TNF-α. In addition, Lr-S treatment activated ERK and p38 MAPK pathways in PBMC. Lipid rafts participation in the reduced production of TNF-α by PBMC induced by Lr-S was verified by lipid rafts disruption with methyl-β-cyclodextrin and the reduction of the Src-tyrosine kinase Lck localization in rafts. Moreover, PBMC pre-treatment with Lck inhibitors blocked the effect of Lr-S on TNF-α production suggesting that activation and mobilization of Lck from lipid rafts would be involved in the modulatory effect of L. reuteri CRL1098. A secreted peptide of 5785 Da would be responsible of the modulatory effect of CRL1098 strain. This study demonstrated for the first time the lipid rafts participation in a response induced by a beneficial bacterium. Also, these results open new possibilities for investigating the molecular mechanisms involved in the interaction of probiotic bacterial extracellular compounds with immune cells.
Fil: Mechoud, Monica Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rodriguez, Ana Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate TNF-α production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced production of TNF-α. In addition, Lr-S treatment activated ERK and p38 MAPK pathways in PBMC. Lipid rafts participation in the reduced production of TNF-α by PBMC induced by Lr-S was verified by lipid rafts disruption with methyl-β-cyclodextrin and the reduction of the Src-tyrosine kinase Lck localization in rafts. Moreover, PBMC pre-treatment with Lck inhibitors blocked the effect of Lr-S on TNF-α production suggesting that activation and mobilization of Lck from lipid rafts would be involved in the modulatory effect of L. reuteri CRL1098. A secreted peptide of 5785 Da would be responsible of the modulatory effect of CRL1098 strain. This study demonstrated for the first time the lipid rafts participation in a response induced by a beneficial bacterium. Also, these results open new possibilities for investigating the molecular mechanisms involved in the interaction of probiotic bacterial extracellular compounds with immune cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25073
Mechoud, Monica Adriana; Mateos, Melina Valeria; Font, Graciela Maria; Villena, Julio Cesar; Salvador, Gabriela Alejandra; et al.; Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts; Elsevier; International Immunopharmacology; 14; 4; 10-2012; 446-453
1567-5769
1878-1705
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25073
identifier_str_mv Mechoud, Monica Adriana; Mateos, Melina Valeria; Font, Graciela Maria; Villena, Julio Cesar; Salvador, Gabriela Alejandra; et al.; Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts; Elsevier; International Immunopharmacology; 14; 4; 10-2012; 446-453
1567-5769
1878-1705
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.intimp.2012.08.020
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567576912002627
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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