Partial characterization of enzymatic activities produced by a wild strain of A. niger

Autores
Martos, María Alicia; Baumann, Alicia; Cruz, Mónica Nancy; Hours, Roque Alberto; Garro, Oscar Alfredo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aspergillus niger, isolated from decay citrus peels in the province of Misiones, was able to produce pectinases by submerged fermentation. The enzymatic extract exhibited polygalacturonase, pectinesterase and lyase activities. Others enzymes capable of degrading cell wall polymers were also detected in the enzymatic extract such as cellulases and xylanases. Polygalacturonase was an endo-polygalacturonase. The enzyme exhibited a maximal activity at pH range between 4.5 to 5.0, was stable in the pH range from 2.5 to 5.5 and remained unchanged when was incubated at temperatures lower than 50 ºC. The fungi produced three PG isoenzymes. The enzymatic extract was able to clarify apple juice. The results observed make the pectinolytic enzymes produced by A. niger appropriate for future application in fruit juice processing industries.
Fil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Baumann, Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Cruz, Mónica Nancy. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Garro, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Chaco Austral; Argentina
Materia
ASPERGILLUS NIGER
PECTINOLYTIC ENZYMES
POLYGALACTURONASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82969
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Partial characterization of enzymatic activities produced by a wild strain of A. nigerMartos, María AliciaBaumann, AliciaCruz, Mónica NancyHours, Roque AlbertoGarro, Oscar AlfredoASPERGILLUS NIGERPECTINOLYTIC ENZYMESPOLYGALACTURONASEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Aspergillus niger, isolated from decay citrus peels in the province of Misiones, was able to produce pectinases by submerged fermentation. The enzymatic extract exhibited polygalacturonase, pectinesterase and lyase activities. Others enzymes capable of degrading cell wall polymers were also detected in the enzymatic extract such as cellulases and xylanases. Polygalacturonase was an endo-polygalacturonase. The enzyme exhibited a maximal activity at pH range between 4.5 to 5.0, was stable in the pH range from 2.5 to 5.5 and remained unchanged when was incubated at temperatures lower than 50 ºC. The fungi produced three PG isoenzymes. The enzymatic extract was able to clarify apple juice. The results observed make the pectinolytic enzymes produced by A. niger appropriate for future application in fruit juice processing industries.Fil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Baumann, Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Cruz, Mónica Nancy. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Garro, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Chaco Austral; ArgentinaSlovak University of Agriculture. Faculty of Biotechnology and Food Science2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82969Martos, María Alicia; Baumann, Alicia; Cruz, Mónica Nancy; Hours, Roque Alberto; Garro, Oscar Alfredo; Partial characterization of enzymatic activities produced by a wild strain of A. niger; Slovak University of Agriculture. Faculty of Biotechnology and Food Science; Journal of Microbiology, Biotechnology and Food Sciences; 2; 3; 12-2012; 917-9321338-5178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jmbfs.org/jmbfs_0146_martos/?issue_id=1574&article_id=6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:36Zoai:ri.conicet.gov.ar:11336/82969instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:36.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Partial characterization of enzymatic activities produced by a wild strain of A. niger
title Partial characterization of enzymatic activities produced by a wild strain of A. niger
spellingShingle Partial characterization of enzymatic activities produced by a wild strain of A. niger
Martos, María Alicia
ASPERGILLUS NIGER
PECTINOLYTIC ENZYMES
POLYGALACTURONASE
title_short Partial characterization of enzymatic activities produced by a wild strain of A. niger
title_full Partial characterization of enzymatic activities produced by a wild strain of A. niger
title_fullStr Partial characterization of enzymatic activities produced by a wild strain of A. niger
title_full_unstemmed Partial characterization of enzymatic activities produced by a wild strain of A. niger
title_sort Partial characterization of enzymatic activities produced by a wild strain of A. niger
dc.creator.none.fl_str_mv Martos, María Alicia
Baumann, Alicia
Cruz, Mónica Nancy
Hours, Roque Alberto
Garro, Oscar Alfredo
author Martos, María Alicia
author_facet Martos, María Alicia
Baumann, Alicia
Cruz, Mónica Nancy
Hours, Roque Alberto
Garro, Oscar Alfredo
author_role author
author2 Baumann, Alicia
Cruz, Mónica Nancy
Hours, Roque Alberto
Garro, Oscar Alfredo
author2_role author
author
author
author
dc.subject.none.fl_str_mv ASPERGILLUS NIGER
PECTINOLYTIC ENZYMES
POLYGALACTURONASE
topic ASPERGILLUS NIGER
PECTINOLYTIC ENZYMES
POLYGALACTURONASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Aspergillus niger, isolated from decay citrus peels in the province of Misiones, was able to produce pectinases by submerged fermentation. The enzymatic extract exhibited polygalacturonase, pectinesterase and lyase activities. Others enzymes capable of degrading cell wall polymers were also detected in the enzymatic extract such as cellulases and xylanases. Polygalacturonase was an endo-polygalacturonase. The enzyme exhibited a maximal activity at pH range between 4.5 to 5.0, was stable in the pH range from 2.5 to 5.5 and remained unchanged when was incubated at temperatures lower than 50 ºC. The fungi produced three PG isoenzymes. The enzymatic extract was able to clarify apple juice. The results observed make the pectinolytic enzymes produced by A. niger appropriate for future application in fruit juice processing industries.
Fil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Baumann, Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Cruz, Mónica Nancy. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Garro, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Chaco Austral; Argentina
description Aspergillus niger, isolated from decay citrus peels in the province of Misiones, was able to produce pectinases by submerged fermentation. The enzymatic extract exhibited polygalacturonase, pectinesterase and lyase activities. Others enzymes capable of degrading cell wall polymers were also detected in the enzymatic extract such as cellulases and xylanases. Polygalacturonase was an endo-polygalacturonase. The enzyme exhibited a maximal activity at pH range between 4.5 to 5.0, was stable in the pH range from 2.5 to 5.5 and remained unchanged when was incubated at temperatures lower than 50 ºC. The fungi produced three PG isoenzymes. The enzymatic extract was able to clarify apple juice. The results observed make the pectinolytic enzymes produced by A. niger appropriate for future application in fruit juice processing industries.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82969
Martos, María Alicia; Baumann, Alicia; Cruz, Mónica Nancy; Hours, Roque Alberto; Garro, Oscar Alfredo; Partial characterization of enzymatic activities produced by a wild strain of A. niger; Slovak University of Agriculture. Faculty of Biotechnology and Food Science; Journal of Microbiology, Biotechnology and Food Sciences; 2; 3; 12-2012; 917-932
1338-5178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82969
identifier_str_mv Martos, María Alicia; Baumann, Alicia; Cruz, Mónica Nancy; Hours, Roque Alberto; Garro, Oscar Alfredo; Partial characterization of enzymatic activities produced by a wild strain of A. niger; Slovak University of Agriculture. Faculty of Biotechnology and Food Science; Journal of Microbiology, Biotechnology and Food Sciences; 2; 3; 12-2012; 917-932
1338-5178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jmbfs.org/jmbfs_0146_martos/?issue_id=1574&article_id=6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Slovak University of Agriculture. Faculty of Biotechnology and Food Science
publisher.none.fl_str_mv Slovak University of Agriculture. Faculty of Biotechnology and Food Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082648762482688
score 13.216834

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