Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid

Autores
Parra, Boris; Tortella, Gonzalo R.; Cuozzo, Sergio Antonio; Martínez, Miguel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Due to their antimicrobial properties Copper nanoparticles (CuNps) have been proposed to be used in agriculture for pest control. Pesticides removal is mainly done by microorganisms, whose genes usually are found in degradative plasmids transferable by conjugation. Our objective was to evaluate if subinhibitory concentrations of CuNps affect the conjugation frequency (CF) of two degradative plasmids, pJP4 and pADP1. CuNps were characterized by scanning electron microscopy with X-ray detector, dynamic light scattering and atomic absorption spectrophotometry. Mating pairs were incubated in liquid media supplemented with subinhibitory concentrations of CuNps (nominal size 40-60 nm) or copper sulphate (CuSO4) as salt form. Transconjugants strains were checked for pesticide degradation and presence of plasmidial genes by PCR. CuNps dissolution in LB broth were quickly (less 3 h), meanwhile in saline medium without organic matter dissolution never pass over 20%. Neither nanoparticles nor copper salt modify the bacterial growth under subinhibitory concentrations assessed. However, under low concentrations of both copper forms (20 µg mL-1), the conjugation frequencies of degradative plasmids were reduced to 90 %. Therefore, our results indicate that ionic copper affect transference of catabolic plasmids as a subinhibitory consequence. The intentional or accidental release of copper to the environment would be affecting the biodegradative potential of microbial communities and inevitably producing as a result a decrease in the pesticides removal.
Fil: Parra, Boris. Universidad de Concepcion; Chile
Fil: Tortella, Gonzalo R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martínez, Miguel. Universidad de Concepción; Chile
Materia
Copper
Nanoparticules
Conjugation
Plasmid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82294
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82294
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmidParra, BorisTortella, Gonzalo R.Cuozzo, Sergio AntonioMartínez, MiguelCopperNanoparticulesConjugationPlasmidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Due to their antimicrobial properties Copper nanoparticles (CuNps) have been proposed to be used in agriculture for pest control. Pesticides removal is mainly done by microorganisms, whose genes usually are found in degradative plasmids transferable by conjugation. Our objective was to evaluate if subinhibitory concentrations of CuNps affect the conjugation frequency (CF) of two degradative plasmids, pJP4 and pADP1. CuNps were characterized by scanning electron microscopy with X-ray detector, dynamic light scattering and atomic absorption spectrophotometry. Mating pairs were incubated in liquid media supplemented with subinhibitory concentrations of CuNps (nominal size 40-60 nm) or copper sulphate (CuSO4) as salt form. Transconjugants strains were checked for pesticide degradation and presence of plasmidial genes by PCR. CuNps dissolution in LB broth were quickly (less 3 h), meanwhile in saline medium without organic matter dissolution never pass over 20%. Neither nanoparticles nor copper salt modify the bacterial growth under subinhibitory concentrations assessed. However, under low concentrations of both copper forms (20 µg mL-1), the conjugation frequencies of degradative plasmids were reduced to 90 %. Therefore, our results indicate that ionic copper affect transference of catabolic plasmids as a subinhibitory consequence. The intentional or accidental release of copper to the environment would be affecting the biodegradative potential of microbial communities and inevitably producing as a result a decrease in the pesticides removal.Fil: Parra, Boris. Universidad de Concepcion; ChileFil: Tortella, Gonzalo R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martínez, Miguel. Universidad de Concepción; ChileAcademic Press Inc Elsevier Science2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82294Parra, Boris; Tortella, Gonzalo R.; Cuozzo, Sergio Antonio; Martínez, Miguel; Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 169; 3; 2-2019; 662-6680147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014765131831193X?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2018.11.057info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:05Zoai:ri.conicet.gov.ar:11336/82294instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:06.126CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
title Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
spellingShingle Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
Parra, Boris
Copper
Nanoparticules
Conjugation
Plasmid
title_short Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
title_full Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
title_fullStr Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
title_full_unstemmed Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
title_sort Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
dc.creator.none.fl_str_mv Parra, Boris
Tortella, Gonzalo R.
Cuozzo, Sergio Antonio
Martínez, Miguel
author Parra, Boris
author_facet Parra, Boris
Tortella, Gonzalo R.
Cuozzo, Sergio Antonio
Martínez, Miguel
author_role author
author2 Tortella, Gonzalo R.
Cuozzo, Sergio Antonio
Martínez, Miguel
author2_role author
author
author
dc.subject.none.fl_str_mv Copper
Nanoparticules
Conjugation
Plasmid
topic Copper
Nanoparticules
Conjugation
Plasmid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Due to their antimicrobial properties Copper nanoparticles (CuNps) have been proposed to be used in agriculture for pest control. Pesticides removal is mainly done by microorganisms, whose genes usually are found in degradative plasmids transferable by conjugation. Our objective was to evaluate if subinhibitory concentrations of CuNps affect the conjugation frequency (CF) of two degradative plasmids, pJP4 and pADP1. CuNps were characterized by scanning electron microscopy with X-ray detector, dynamic light scattering and atomic absorption spectrophotometry. Mating pairs were incubated in liquid media supplemented with subinhibitory concentrations of CuNps (nominal size 40-60 nm) or copper sulphate (CuSO4) as salt form. Transconjugants strains were checked for pesticide degradation and presence of plasmidial genes by PCR. CuNps dissolution in LB broth were quickly (less 3 h), meanwhile in saline medium without organic matter dissolution never pass over 20%. Neither nanoparticles nor copper salt modify the bacterial growth under subinhibitory concentrations assessed. However, under low concentrations of both copper forms (20 µg mL-1), the conjugation frequencies of degradative plasmids were reduced to 90 %. Therefore, our results indicate that ionic copper affect transference of catabolic plasmids as a subinhibitory consequence. The intentional or accidental release of copper to the environment would be affecting the biodegradative potential of microbial communities and inevitably producing as a result a decrease in the pesticides removal.
Fil: Parra, Boris. Universidad de Concepcion; Chile
Fil: Tortella, Gonzalo R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martínez, Miguel. Universidad de Concepción; Chile
description Due to their antimicrobial properties Copper nanoparticles (CuNps) have been proposed to be used in agriculture for pest control. Pesticides removal is mainly done by microorganisms, whose genes usually are found in degradative plasmids transferable by conjugation. Our objective was to evaluate if subinhibitory concentrations of CuNps affect the conjugation frequency (CF) of two degradative plasmids, pJP4 and pADP1. CuNps were characterized by scanning electron microscopy with X-ray detector, dynamic light scattering and atomic absorption spectrophotometry. Mating pairs were incubated in liquid media supplemented with subinhibitory concentrations of CuNps (nominal size 40-60 nm) or copper sulphate (CuSO4) as salt form. Transconjugants strains were checked for pesticide degradation and presence of plasmidial genes by PCR. CuNps dissolution in LB broth were quickly (less 3 h), meanwhile in saline medium without organic matter dissolution never pass over 20%. Neither nanoparticles nor copper salt modify the bacterial growth under subinhibitory concentrations assessed. However, under low concentrations of both copper forms (20 µg mL-1), the conjugation frequencies of degradative plasmids were reduced to 90 %. Therefore, our results indicate that ionic copper affect transference of catabolic plasmids as a subinhibitory consequence. The intentional or accidental release of copper to the environment would be affecting the biodegradative potential of microbial communities and inevitably producing as a result a decrease in the pesticides removal.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82294
Parra, Boris; Tortella, Gonzalo R.; Cuozzo, Sergio Antonio; Martínez, Miguel; Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 169; 3; 2-2019; 662-668
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82294
identifier_str_mv Parra, Boris; Tortella, Gonzalo R.; Cuozzo, Sergio Antonio; Martínez, Miguel; Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 169; 3; 2-2019; 662-668
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014765131831193X?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2018.11.057
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613800110587904
score 13.070432

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