Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts

Autores
Sampietro, Diego Alejandro; Mercado, Maria Ines; Aristimuño Ficoseco, Maria Eugenia del Milagro; Ponessa, Graciela; Vattuone, Marta Amelia; Catalan, Cesar Atilio Nazareno
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The trees Schinopsis lorentzii and S. marginata produce alk(en)ylcatechols, also known as urushiols, which function in plant defense as antifungals, antifeedants and cause contact dermatitis in human beings. Here, we propose the use of the diazonium salts Fast Blue B ½ZnCl2 and Fast Blue RR ZnCl2 for the in situ histochemical localization of urushiols in stems and leaflets of S. lorentzii and S. marginata. The main surface and internal anatomical features of the aerial plant parts were visualized with cresyl violet and astra blue safranin. Extracts from leaflets and stems were separated by TLC and revealed with aqueous solutions of 0.5% Fast Blue B ½ZnCl2 in 5% acetic acid (FBB), 0.5% Fast Blue RR ZnCl2 in 5% KOH (FBRR) and 5% FeCl3. Fresh cross sections of leaflets and stems were also stained with these chromogenic reagents. Mature leaflets and stems showed few glandular trichomes in their surfaces. Well developed phloem schizogenous ducts were uniformly distributed in the leaflet mesophylls and radially organized in the cross sections of the stems. TLC of the extracts revealed with FBB, FBRR and 5% FeCl3 gave bluish black spots for urushiols. Very few constituents of the extracts other than the urushiols revealed the same colour after spraying with the diazonium salts. FBB and FBRR indicated the presence of urushiols in both the cavity of the phloem schizogenous ducts and their surrounding epithelial cells. Urushiols were not visualized neither in the cuticle of the aerial parts nor in the glandular trichomes. FBB was coupled to more individual polyphenol species than FBRR whereas FeCl3 was by far a less specific chromogenic reagent. Nevertheless, FBRR was more selective towards the urushiols than FBB. The exposure to urushiols should be possible only after the breakdown of leaflets and stems of Schinopsis trees.
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Mercado, Maria Ines. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina
Fil: Aristimuño Ficoseco, Maria Eugenia del Milagro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Ponessa, Graciela. Fundación Miguel Lillo; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Materia
Alk(En)Ylcatechols
Diazonium Salts
Histochemistry
Schinopsis
Urushiols
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67484
Acceder
id CONICETDig_0b688fbacce4c655a59fddbb4f8f434f
oai_identifier_str oai:ri.conicet.gov.ar:11336/67484
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium saltsSampietro, Diego AlejandroMercado, Maria InesAristimuño Ficoseco, Maria Eugenia del MilagroPonessa, GracielaVattuone, Marta AmeliaCatalan, Cesar Atilio NazarenoAlk(En)YlcatecholsDiazonium SaltsHistochemistrySchinopsisUrushiolshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The trees Schinopsis lorentzii and S. marginata produce alk(en)ylcatechols, also known as urushiols, which function in plant defense as antifungals, antifeedants and cause contact dermatitis in human beings. Here, we propose the use of the diazonium salts Fast Blue B ½ZnCl2 and Fast Blue RR ZnCl2 for the in situ histochemical localization of urushiols in stems and leaflets of S. lorentzii and S. marginata. The main surface and internal anatomical features of the aerial plant parts were visualized with cresyl violet and astra blue safranin. Extracts from leaflets and stems were separated by TLC and revealed with aqueous solutions of 0.5% Fast Blue B ½ZnCl2 in 5% acetic acid (FBB), 0.5% Fast Blue RR ZnCl2 in 5% KOH (FBRR) and 5% FeCl3. Fresh cross sections of leaflets and stems were also stained with these chromogenic reagents. Mature leaflets and stems showed few glandular trichomes in their surfaces. Well developed phloem schizogenous ducts were uniformly distributed in the leaflet mesophylls and radially organized in the cross sections of the stems. TLC of the extracts revealed with FBB, FBRR and 5% FeCl3 gave bluish black spots for urushiols. Very few constituents of the extracts other than the urushiols revealed the same colour after spraying with the diazonium salts. FBB and FBRR indicated the presence of urushiols in both the cavity of the phloem schizogenous ducts and their surrounding epithelial cells. Urushiols were not visualized neither in the cuticle of the aerial parts nor in the glandular trichomes. FBB was coupled to more individual polyphenol species than FBRR whereas FeCl3 was by far a less specific chromogenic reagent. Nevertheless, FBRR was more selective towards the urushiols than FBB. The exposure to urushiols should be possible only after the breakdown of leaflets and stems of Schinopsis trees.Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Mercado, Maria Ines. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Aristimuño Ficoseco, Maria Eugenia del Milagro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Ponessa, Graciela. Fundación Miguel Lillo; ArgentinaFil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaElsevier Gmbh2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67484Sampietro, Diego Alejandro; Mercado, Maria Ines; Aristimuño Ficoseco, Maria Eugenia del Milagro; Ponessa, Graciela; Vattuone, Marta Amelia; et al.; Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts; Elsevier Gmbh; Flora; 236-237; 11-2017; 25-320367-2530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.flora.2017.09.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0367253017333157info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:54Zoai:ri.conicet.gov.ar:11336/67484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:54.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
title Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
spellingShingle Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
Sampietro, Diego Alejandro
Alk(En)Ylcatechols
Diazonium Salts
Histochemistry
Schinopsis
Urushiols
title_short Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
title_full Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
title_fullStr Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
title_full_unstemmed Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
title_sort Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts
dc.creator.none.fl_str_mv Sampietro, Diego Alejandro
Mercado, Maria Ines
Aristimuño Ficoseco, Maria Eugenia del Milagro
Ponessa, Graciela
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
author Sampietro, Diego Alejandro
author_facet Sampietro, Diego Alejandro
Mercado, Maria Ines
Aristimuño Ficoseco, Maria Eugenia del Milagro
Ponessa, Graciela
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
author_role author
author2 Mercado, Maria Ines
Aristimuño Ficoseco, Maria Eugenia del Milagro
Ponessa, Graciela
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Alk(En)Ylcatechols
Diazonium Salts
Histochemistry
Schinopsis
Urushiols
topic Alk(En)Ylcatechols
Diazonium Salts
Histochemistry
Schinopsis
Urushiols
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The trees Schinopsis lorentzii and S. marginata produce alk(en)ylcatechols, also known as urushiols, which function in plant defense as antifungals, antifeedants and cause contact dermatitis in human beings. Here, we propose the use of the diazonium salts Fast Blue B ½ZnCl2 and Fast Blue RR ZnCl2 for the in situ histochemical localization of urushiols in stems and leaflets of S. lorentzii and S. marginata. The main surface and internal anatomical features of the aerial plant parts were visualized with cresyl violet and astra blue safranin. Extracts from leaflets and stems were separated by TLC and revealed with aqueous solutions of 0.5% Fast Blue B ½ZnCl2 in 5% acetic acid (FBB), 0.5% Fast Blue RR ZnCl2 in 5% KOH (FBRR) and 5% FeCl3. Fresh cross sections of leaflets and stems were also stained with these chromogenic reagents. Mature leaflets and stems showed few glandular trichomes in their surfaces. Well developed phloem schizogenous ducts were uniformly distributed in the leaflet mesophylls and radially organized in the cross sections of the stems. TLC of the extracts revealed with FBB, FBRR and 5% FeCl3 gave bluish black spots for urushiols. Very few constituents of the extracts other than the urushiols revealed the same colour after spraying with the diazonium salts. FBB and FBRR indicated the presence of urushiols in both the cavity of the phloem schizogenous ducts and their surrounding epithelial cells. Urushiols were not visualized neither in the cuticle of the aerial parts nor in the glandular trichomes. FBB was coupled to more individual polyphenol species than FBRR whereas FeCl3 was by far a less specific chromogenic reagent. Nevertheless, FBRR was more selective towards the urushiols than FBB. The exposure to urushiols should be possible only after the breakdown of leaflets and stems of Schinopsis trees.
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Mercado, Maria Ines. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina
Fil: Aristimuño Ficoseco, Maria Eugenia del Milagro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Ponessa, Graciela. Fundación Miguel Lillo; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
description The trees Schinopsis lorentzii and S. marginata produce alk(en)ylcatechols, also known as urushiols, which function in plant defense as antifungals, antifeedants and cause contact dermatitis in human beings. Here, we propose the use of the diazonium salts Fast Blue B ½ZnCl2 and Fast Blue RR ZnCl2 for the in situ histochemical localization of urushiols in stems and leaflets of S. lorentzii and S. marginata. The main surface and internal anatomical features of the aerial plant parts were visualized with cresyl violet and astra blue safranin. Extracts from leaflets and stems were separated by TLC and revealed with aqueous solutions of 0.5% Fast Blue B ½ZnCl2 in 5% acetic acid (FBB), 0.5% Fast Blue RR ZnCl2 in 5% KOH (FBRR) and 5% FeCl3. Fresh cross sections of leaflets and stems were also stained with these chromogenic reagents. Mature leaflets and stems showed few glandular trichomes in their surfaces. Well developed phloem schizogenous ducts were uniformly distributed in the leaflet mesophylls and radially organized in the cross sections of the stems. TLC of the extracts revealed with FBB, FBRR and 5% FeCl3 gave bluish black spots for urushiols. Very few constituents of the extracts other than the urushiols revealed the same colour after spraying with the diazonium salts. FBB and FBRR indicated the presence of urushiols in both the cavity of the phloem schizogenous ducts and their surrounding epithelial cells. Urushiols were not visualized neither in the cuticle of the aerial parts nor in the glandular trichomes. FBB was coupled to more individual polyphenol species than FBRR whereas FeCl3 was by far a less specific chromogenic reagent. Nevertheless, FBRR was more selective towards the urushiols than FBB. The exposure to urushiols should be possible only after the breakdown of leaflets and stems of Schinopsis trees.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67484
Sampietro, Diego Alejandro; Mercado, Maria Ines; Aristimuño Ficoseco, Maria Eugenia del Milagro; Ponessa, Graciela; Vattuone, Marta Amelia; et al.; Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts; Elsevier Gmbh; Flora; 236-237; 11-2017; 25-32
0367-2530
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67484
identifier_str_mv Sampietro, Diego Alejandro; Mercado, Maria Ines; Aristimuño Ficoseco, Maria Eugenia del Milagro; Ponessa, Graciela; Vattuone, Marta Amelia; et al.; Histochemical localization of urushiols in stems and leaflets of Schinopsis lorentzii and S. marginata using diazonium salts; Elsevier Gmbh; Flora; 236-237; 11-2017; 25-32
0367-2530
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.flora.2017.09.006
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0367253017333157
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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