Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction

Autores
Veggetti, Mariela Iris; de Lorenzo, Silvana; Cassone, Julieta; Losavio, Adriana Silvia; Muchnik, Salomon
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The diagnosis of bipolar disease frequently requires a long time since the age of onset, especially because the disease ismisdiagnosed with schizophrenia. The aim of the present work was to investigate whether sera from bipolar patients have an activesubstance that allows making a fast identification of the disease.Methods: Sera from healthy volunteers, euthymic and non-stabilized bipolar patients, and schizophrenic patients were passively transferredinto CF1 mice and after 2 day injections, MEPP frequency from diaphragm muscles was recorded. The same procedure was performedwith sera fraction of high and low MW (cut-off 3000).Results: Sera from non-stabilized bipolar patients induced a decreased MEPP frequency and occluded the presynaptic inhibitory effect ofthe specific adenosine A1 receptor agonist 2-chloro-N6-cyclopentyl-adenosine (CCPA) in the recipient mice, while in the euthymic bipolargroup spontaneous secretion reached control values although the action of CCPA was still prevented. Similar results were obtained withlow MW sera fraction from euthymic and non-stabilized bipolar patients. The addition of adenosine deaminase to the sera fraction preventedthe modification of spontaneous ACh release. In mice injected with sera from schizophrenic patients, MEPP frequency was withincontrol values and CCPA induced its typical inhibitory action.Conclusions: These results indicate that bipolar patients contain in their blood an active substance compatible with adenosine, which wasable to modify spontaneous ACh release in the recipient mice. This effect was not observed with sera from healthy volunteers and schizophrenicpatients. The increase of adenosine concentration may result from synaptic hyperactivity that presumably plays a role in thesymptoms of bipolar disorder and/or may derive from peripheral cells through a more general mechanism.Significance: The different results obtained with bipolar and schizophrenic sera raise the possibility that the passive transfer model couldbe used as a diagnostic test in the future.
Fil: Veggetti, Mariela Iris. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Lorenzo, Silvana. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Cassone, Julieta. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Losavio, Adriana Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Muchnik, Salomon. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BIPOLAR DISORDER
SCHIZOPHRENIA
ADENOSINE
MAMMALIAN NEUROMUSCULAR JUNCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107557
Acceder
id CONICETDig_0a459b3b08667aee958ae66ee053274b
oai_identifier_str oai:ri.conicet.gov.ar:11336/107557
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junctionVeggetti, Mariela Irisde Lorenzo, SilvanaCassone, JulietaLosavio, Adriana SilviaMuchnik, SalomonBIPOLAR DISORDERSCHIZOPHRENIAADENOSINEMAMMALIAN NEUROMUSCULAR JUNCTIONhttps://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1Objective: The diagnosis of bipolar disease frequently requires a long time since the age of onset, especially because the disease ismisdiagnosed with schizophrenia. The aim of the present work was to investigate whether sera from bipolar patients have an activesubstance that allows making a fast identification of the disease.Methods: Sera from healthy volunteers, euthymic and non-stabilized bipolar patients, and schizophrenic patients were passively transferredinto CF1 mice and after 2 day injections, MEPP frequency from diaphragm muscles was recorded. The same procedure was performedwith sera fraction of high and low MW (cut-off 3000).Results: Sera from non-stabilized bipolar patients induced a decreased MEPP frequency and occluded the presynaptic inhibitory effect ofthe specific adenosine A1 receptor agonist 2-chloro-N6-cyclopentyl-adenosine (CCPA) in the recipient mice, while in the euthymic bipolargroup spontaneous secretion reached control values although the action of CCPA was still prevented. Similar results were obtained withlow MW sera fraction from euthymic and non-stabilized bipolar patients. The addition of adenosine deaminase to the sera fraction preventedthe modification of spontaneous ACh release. In mice injected with sera from schizophrenic patients, MEPP frequency was withincontrol values and CCPA induced its typical inhibitory action.Conclusions: These results indicate that bipolar patients contain in their blood an active substance compatible with adenosine, which wasable to modify spontaneous ACh release in the recipient mice. This effect was not observed with sera from healthy volunteers and schizophrenicpatients. The increase of adenosine concentration may result from synaptic hyperactivity that presumably plays a role in thesymptoms of bipolar disorder and/or may derive from peripheral cells through a more general mechanism.Significance: The different results obtained with bipolar and schizophrenic sera raise the possibility that the passive transfer model couldbe used as a diagnostic test in the future.Fil: Veggetti, Mariela Iris. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Lorenzo, Silvana. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Cassone, Julieta. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Losavio, Adriana Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Muchnik, Salomon. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107557Veggetti, Mariela Iris; de Lorenzo, Silvana; Cassone, Julieta; Losavio, Adriana Silvia; Muchnik, Salomon; Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction; Elsevier Ireland; Clinical Neurophysiology; 117; 12; 12-2006; 2645-26521388-2457CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1388245706014052info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clinph.2006.08.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:16:01Zoai:ri.conicet.gov.ar:11336/107557instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:16:01.908CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
title Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
spellingShingle Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
Veggetti, Mariela Iris
BIPOLAR DISORDER
SCHIZOPHRENIA
ADENOSINE
MAMMALIAN NEUROMUSCULAR JUNCTION
title_short Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
title_full Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
title_fullStr Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
title_full_unstemmed Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
title_sort Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
dc.creator.none.fl_str_mv Veggetti, Mariela Iris
de Lorenzo, Silvana
Cassone, Julieta
Losavio, Adriana Silvia
Muchnik, Salomon
author Veggetti, Mariela Iris
author_facet Veggetti, Mariela Iris
de Lorenzo, Silvana
Cassone, Julieta
Losavio, Adriana Silvia
Muchnik, Salomon
author_role author
author2 de Lorenzo, Silvana
Cassone, Julieta
Losavio, Adriana Silvia
Muchnik, Salomon
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIPOLAR DISORDER
SCHIZOPHRENIA
ADENOSINE
MAMMALIAN NEUROMUSCULAR JUNCTION
topic BIPOLAR DISORDER
SCHIZOPHRENIA
ADENOSINE
MAMMALIAN NEUROMUSCULAR JUNCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
dc.description.none.fl_txt_mv Objective: The diagnosis of bipolar disease frequently requires a long time since the age of onset, especially because the disease ismisdiagnosed with schizophrenia. The aim of the present work was to investigate whether sera from bipolar patients have an activesubstance that allows making a fast identification of the disease.Methods: Sera from healthy volunteers, euthymic and non-stabilized bipolar patients, and schizophrenic patients were passively transferredinto CF1 mice and after 2 day injections, MEPP frequency from diaphragm muscles was recorded. The same procedure was performedwith sera fraction of high and low MW (cut-off 3000).Results: Sera from non-stabilized bipolar patients induced a decreased MEPP frequency and occluded the presynaptic inhibitory effect ofthe specific adenosine A1 receptor agonist 2-chloro-N6-cyclopentyl-adenosine (CCPA) in the recipient mice, while in the euthymic bipolargroup spontaneous secretion reached control values although the action of CCPA was still prevented. Similar results were obtained withlow MW sera fraction from euthymic and non-stabilized bipolar patients. The addition of adenosine deaminase to the sera fraction preventedthe modification of spontaneous ACh release. In mice injected with sera from schizophrenic patients, MEPP frequency was withincontrol values and CCPA induced its typical inhibitory action.Conclusions: These results indicate that bipolar patients contain in their blood an active substance compatible with adenosine, which wasable to modify spontaneous ACh release in the recipient mice. This effect was not observed with sera from healthy volunteers and schizophrenicpatients. The increase of adenosine concentration may result from synaptic hyperactivity that presumably plays a role in thesymptoms of bipolar disorder and/or may derive from peripheral cells through a more general mechanism.Significance: The different results obtained with bipolar and schizophrenic sera raise the possibility that the passive transfer model couldbe used as a diagnostic test in the future.
Fil: Veggetti, Mariela Iris. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Lorenzo, Silvana. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Cassone, Julieta. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Losavio, Adriana Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Muchnik, Salomon. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective: The diagnosis of bipolar disease frequently requires a long time since the age of onset, especially because the disease ismisdiagnosed with schizophrenia. The aim of the present work was to investigate whether sera from bipolar patients have an activesubstance that allows making a fast identification of the disease.Methods: Sera from healthy volunteers, euthymic and non-stabilized bipolar patients, and schizophrenic patients were passively transferredinto CF1 mice and after 2 day injections, MEPP frequency from diaphragm muscles was recorded. The same procedure was performedwith sera fraction of high and low MW (cut-off 3000).Results: Sera from non-stabilized bipolar patients induced a decreased MEPP frequency and occluded the presynaptic inhibitory effect ofthe specific adenosine A1 receptor agonist 2-chloro-N6-cyclopentyl-adenosine (CCPA) in the recipient mice, while in the euthymic bipolargroup spontaneous secretion reached control values although the action of CCPA was still prevented. Similar results were obtained withlow MW sera fraction from euthymic and non-stabilized bipolar patients. The addition of adenosine deaminase to the sera fraction preventedthe modification of spontaneous ACh release. In mice injected with sera from schizophrenic patients, MEPP frequency was withincontrol values and CCPA induced its typical inhibitory action.Conclusions: These results indicate that bipolar patients contain in their blood an active substance compatible with adenosine, which wasable to modify spontaneous ACh release in the recipient mice. This effect was not observed with sera from healthy volunteers and schizophrenicpatients. The increase of adenosine concentration may result from synaptic hyperactivity that presumably plays a role in thesymptoms of bipolar disorder and/or may derive from peripheral cells through a more general mechanism.Significance: The different results obtained with bipolar and schizophrenic sera raise the possibility that the passive transfer model couldbe used as a diagnostic test in the future.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107557
Veggetti, Mariela Iris; de Lorenzo, Silvana; Cassone, Julieta; Losavio, Adriana Silvia; Muchnik, Salomon; Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction; Elsevier Ireland; Clinical Neurophysiology; 117; 12; 12-2006; 2645-2652
1388-2457
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107557
identifier_str_mv Veggetti, Mariela Iris; de Lorenzo, Silvana; Cassone, Julieta; Losavio, Adriana Silvia; Muchnik, Salomon; Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction; Elsevier Ireland; Clinical Neurophysiology; 117; 12; 12-2006; 2645-2652
1388-2457
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1388245706014052
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clinph.2006.08.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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