Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption

Autores
Soldano, Sara; Bonanomi, Marcella; Aramini, Tecla; Moyano, Andrea del Huerto; Garbelli, Anna; Croce, Anna Cleta; Weththimuni, Maduka L.; Vaghi, Patrizia; Puggioli, Arianna; Gomulski, Ludvik M.; Gaglio, Daniela; Scolari, Francesca
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Urban aquatic environments are increasingly contaminated with micro- and nanoplastics (MNLPs), posing risks to biodiversity and human health. These environments are ideal breeding sites for larvae of the Asian tiger mosquito Aedes albopictus, a key arbovirus vector. While plastic pollution is a global concern, physiological and metabolic consequences of MNPL-exposure in mosquitoes remain poorly understood. Combining epifluorescence and confocal imaging, mosquito life-history parameter assessment, and high-throughput metabolomic profiling, we investigated the effects of polystyrene MNPLs on Ae. albopictus. We demonstrated that ingested MNPLs cross the larval gut barrier, persist in various tissues, and are retained through development. Exposure did not significantly affect pre-imaginal survival or development time, but reduced larval body weight and caused profound metabolic alterations. Metabolomic analyses revealed downregulation of central carbon metabolism, particularly glycolysis and pentose phosphate pathway, alongside amino acid alterations linked to stress responses. Notably, MNPL-exposure also affected juvenile hormone biosynthesis, suggesting endocrine disruption. This trend was observed in both NP- and MP-exposed larvae, with a greater number of differentially regulated metabolites following MP-treatment. Interestingly, TCA cycle dysregulation was more pronounced in NP-exposed larvae, whereas perturbations in glutathione metabolism, amino sugar metabolism and nitrogen excretion were associated with MP-exposure. These findings highlight the metabolic and physiological consequences of MNPL-exposure, with potential implications for mosquito ecology, vector capacity, and environmental dissemination of plastic contaminants. Given the role of Ae. albopictus in disease transmission and its adaptation to urban habitats, further research is needed to explore the long-term ecological and epidemiological consequences of plastic pollution on mosquito populations.
Fil: Soldano, Sara. Consiglio Nazionale delle Ricerche; Italia. Universita degli Studi di Pavia; Italia
Fil: Bonanomi, Marcella. Consiglio Nazionale delle Ricerche; Italia
Fil: Aramini, Tecla. Consiglio Nazionale delle Ricerche; Italia
Fil: Moyano, Andrea del Huerto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consiglio Nazionale delle Ricerche; Italia
Fil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; Italia
Fil: Weththimuni, Maduka L.. Universita degli Studi di Pavia; Italia
Fil: Vaghi, Patrizia. Universita degli Studi di Pavia; Italia
Fil: Puggioli, Arianna. Centro Agricoltura Ambiente; Italia
Fil: Gomulski, Ludvik M.. Universita degli Studi di Pavia; Italia
Fil: Gaglio, Daniela. Consiglio Nazionale delle Ricerche; Italia
Fil: Scolari, Francesca. Consiglio Nazionale delle Ricerche; Italia
Materia
Asian tiger mosquito
Fluorescence confocal imaging
Liquid chromatography-mass spectrometry
Metabolomics
Nanoplastic
Ontogenic transfer
Polystyrene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275576
Acceder
id CONICETDig_0a4451ded0ab9bd166de30d259b83069
oai_identifier_str oai:ri.conicet.gov.ar:11336/275576
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruptionSoldano, SaraBonanomi, MarcellaAramini, TeclaMoyano, Andrea del HuertoGarbelli, AnnaCroce, Anna CletaWeththimuni, Maduka L.Vaghi, PatriziaPuggioli, AriannaGomulski, Ludvik M.Gaglio, DanielaScolari, FrancescaAsian tiger mosquitoFluorescence confocal imagingLiquid chromatography-mass spectrometryMetabolomicsNanoplasticOntogenic transferPolystyrenehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Urban aquatic environments are increasingly contaminated with micro- and nanoplastics (MNLPs), posing risks to biodiversity and human health. These environments are ideal breeding sites for larvae of the Asian tiger mosquito Aedes albopictus, a key arbovirus vector. While plastic pollution is a global concern, physiological and metabolic consequences of MNPL-exposure in mosquitoes remain poorly understood. Combining epifluorescence and confocal imaging, mosquito life-history parameter assessment, and high-throughput metabolomic profiling, we investigated the effects of polystyrene MNPLs on Ae. albopictus. We demonstrated that ingested MNPLs cross the larval gut barrier, persist in various tissues, and are retained through development. Exposure did not significantly affect pre-imaginal survival or development time, but reduced larval body weight and caused profound metabolic alterations. Metabolomic analyses revealed downregulation of central carbon metabolism, particularly glycolysis and pentose phosphate pathway, alongside amino acid alterations linked to stress responses. Notably, MNPL-exposure also affected juvenile hormone biosynthesis, suggesting endocrine disruption. This trend was observed in both NP- and MP-exposed larvae, with a greater number of differentially regulated metabolites following MP-treatment. Interestingly, TCA cycle dysregulation was more pronounced in NP-exposed larvae, whereas perturbations in glutathione metabolism, amino sugar metabolism and nitrogen excretion were associated with MP-exposure. These findings highlight the metabolic and physiological consequences of MNPL-exposure, with potential implications for mosquito ecology, vector capacity, and environmental dissemination of plastic contaminants. Given the role of Ae. albopictus in disease transmission and its adaptation to urban habitats, further research is needed to explore the long-term ecological and epidemiological consequences of plastic pollution on mosquito populations.Fil: Soldano, Sara. Consiglio Nazionale delle Ricerche; Italia. Universita degli Studi di Pavia; ItaliaFil: Bonanomi, Marcella. Consiglio Nazionale delle Ricerche; ItaliaFil: Aramini, Tecla. Consiglio Nazionale delle Ricerche; ItaliaFil: Moyano, Andrea del Huerto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consiglio Nazionale delle Ricerche; ItaliaFil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; ItaliaFil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; ItaliaFil: Weththimuni, Maduka L.. Universita degli Studi di Pavia; ItaliaFil: Vaghi, Patrizia. Universita degli Studi di Pavia; ItaliaFil: Puggioli, Arianna. Centro Agricoltura Ambiente; ItaliaFil: Gomulski, Ludvik M.. Universita degli Studi di Pavia; ItaliaFil: Gaglio, Daniela. Consiglio Nazionale delle Ricerche; ItaliaFil: Scolari, Francesca. Consiglio Nazionale delle Ricerche; ItaliaElsevier2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275576Soldano, Sara; Bonanomi, Marcella; Aramini, Tecla; Moyano, Andrea del Huerto; Garbelli, Anna; et al.; Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption; Elsevier; Science of the Total Environment; 1002; 11-2025; 1-190048-9697CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S004896972502087Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2025.180447info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T11:52:48Zoai:ri.conicet.gov.ar:11336/275576instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 11:52:49.217CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
title Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
spellingShingle Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
Soldano, Sara
Asian tiger mosquito
Fluorescence confocal imaging
Liquid chromatography-mass spectrometry
Metabolomics
Nanoplastic
Ontogenic transfer
Polystyrene
title_short Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
title_full Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
title_fullStr Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
title_full_unstemmed Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
title_sort Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption
dc.creator.none.fl_str_mv Soldano, Sara
Bonanomi, Marcella
Aramini, Tecla
Moyano, Andrea del Huerto
Garbelli, Anna
Croce, Anna Cleta
Weththimuni, Maduka L.
Vaghi, Patrizia
Puggioli, Arianna
Gomulski, Ludvik M.
Gaglio, Daniela
Scolari, Francesca
author Soldano, Sara
author_facet Soldano, Sara
Bonanomi, Marcella
Aramini, Tecla
Moyano, Andrea del Huerto
Garbelli, Anna
Croce, Anna Cleta
Weththimuni, Maduka L.
Vaghi, Patrizia
Puggioli, Arianna
Gomulski, Ludvik M.
Gaglio, Daniela
Scolari, Francesca
author_role author
author2 Bonanomi, Marcella
Aramini, Tecla
Moyano, Andrea del Huerto
Garbelli, Anna
Croce, Anna Cleta
Weththimuni, Maduka L.
Vaghi, Patrizia
Puggioli, Arianna
Gomulski, Ludvik M.
Gaglio, Daniela
Scolari, Francesca
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Asian tiger mosquito
Fluorescence confocal imaging
Liquid chromatography-mass spectrometry
Metabolomics
Nanoplastic
Ontogenic transfer
Polystyrene
topic Asian tiger mosquito
Fluorescence confocal imaging
Liquid chromatography-mass spectrometry
Metabolomics
Nanoplastic
Ontogenic transfer
Polystyrene
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Urban aquatic environments are increasingly contaminated with micro- and nanoplastics (MNLPs), posing risks to biodiversity and human health. These environments are ideal breeding sites for larvae of the Asian tiger mosquito Aedes albopictus, a key arbovirus vector. While plastic pollution is a global concern, physiological and metabolic consequences of MNPL-exposure in mosquitoes remain poorly understood. Combining epifluorescence and confocal imaging, mosquito life-history parameter assessment, and high-throughput metabolomic profiling, we investigated the effects of polystyrene MNPLs on Ae. albopictus. We demonstrated that ingested MNPLs cross the larval gut barrier, persist in various tissues, and are retained through development. Exposure did not significantly affect pre-imaginal survival or development time, but reduced larval body weight and caused profound metabolic alterations. Metabolomic analyses revealed downregulation of central carbon metabolism, particularly glycolysis and pentose phosphate pathway, alongside amino acid alterations linked to stress responses. Notably, MNPL-exposure also affected juvenile hormone biosynthesis, suggesting endocrine disruption. This trend was observed in both NP- and MP-exposed larvae, with a greater number of differentially regulated metabolites following MP-treatment. Interestingly, TCA cycle dysregulation was more pronounced in NP-exposed larvae, whereas perturbations in glutathione metabolism, amino sugar metabolism and nitrogen excretion were associated with MP-exposure. These findings highlight the metabolic and physiological consequences of MNPL-exposure, with potential implications for mosquito ecology, vector capacity, and environmental dissemination of plastic contaminants. Given the role of Ae. albopictus in disease transmission and its adaptation to urban habitats, further research is needed to explore the long-term ecological and epidemiological consequences of plastic pollution on mosquito populations.
Fil: Soldano, Sara. Consiglio Nazionale delle Ricerche; Italia. Universita degli Studi di Pavia; Italia
Fil: Bonanomi, Marcella. Consiglio Nazionale delle Ricerche; Italia
Fil: Aramini, Tecla. Consiglio Nazionale delle Ricerche; Italia
Fil: Moyano, Andrea del Huerto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consiglio Nazionale delle Ricerche; Italia
Fil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; Italia
Fil: Weththimuni, Maduka L.. Universita degli Studi di Pavia; Italia
Fil: Vaghi, Patrizia. Universita degli Studi di Pavia; Italia
Fil: Puggioli, Arianna. Centro Agricoltura Ambiente; Italia
Fil: Gomulski, Ludvik M.. Universita degli Studi di Pavia; Italia
Fil: Gaglio, Daniela. Consiglio Nazionale delle Ricerche; Italia
Fil: Scolari, Francesca. Consiglio Nazionale delle Ricerche; Italia
description Urban aquatic environments are increasingly contaminated with micro- and nanoplastics (MNLPs), posing risks to biodiversity and human health. These environments are ideal breeding sites for larvae of the Asian tiger mosquito Aedes albopictus, a key arbovirus vector. While plastic pollution is a global concern, physiological and metabolic consequences of MNPL-exposure in mosquitoes remain poorly understood. Combining epifluorescence and confocal imaging, mosquito life-history parameter assessment, and high-throughput metabolomic profiling, we investigated the effects of polystyrene MNPLs on Ae. albopictus. We demonstrated that ingested MNPLs cross the larval gut barrier, persist in various tissues, and are retained through development. Exposure did not significantly affect pre-imaginal survival or development time, but reduced larval body weight and caused profound metabolic alterations. Metabolomic analyses revealed downregulation of central carbon metabolism, particularly glycolysis and pentose phosphate pathway, alongside amino acid alterations linked to stress responses. Notably, MNPL-exposure also affected juvenile hormone biosynthesis, suggesting endocrine disruption. This trend was observed in both NP- and MP-exposed larvae, with a greater number of differentially regulated metabolites following MP-treatment. Interestingly, TCA cycle dysregulation was more pronounced in NP-exposed larvae, whereas perturbations in glutathione metabolism, amino sugar metabolism and nitrogen excretion were associated with MP-exposure. These findings highlight the metabolic and physiological consequences of MNPL-exposure, with potential implications for mosquito ecology, vector capacity, and environmental dissemination of plastic contaminants. Given the role of Ae. albopictus in disease transmission and its adaptation to urban habitats, further research is needed to explore the long-term ecological and epidemiological consequences of plastic pollution on mosquito populations.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275576
Soldano, Sara; Bonanomi, Marcella; Aramini, Tecla; Moyano, Andrea del Huerto; Garbelli, Anna; et al.; Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption; Elsevier; Science of the Total Environment; 1002; 11-2025; 1-19
0048-9697
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275576
identifier_str_mv Soldano, Sara; Bonanomi, Marcella; Aramini, Tecla; Moyano, Andrea del Huerto; Garbelli, Anna; et al.; Tracking micro- and nanoplastics in Aedes albopictus: From ingestion to metabolic disruption; Elsevier; Science of the Total Environment; 1002; 11-2025; 1-19
0048-9697
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S004896972502087X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2025.180447
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.113929

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