Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America

Autores
Cardozo, Rubén Marino; Panzera, Francisco; Gentile, Alberto Gerónimo; Segura, Maria Asuncion; Pérez, Ruben; Diaz, Renee Analia; Basombrío, Miguel Ángel Manuel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An outbreak of pyrethroid resistance was recently detected in Triatoma infestans from northern Argentina. To analyze the inheritance of the resistant phenotype, we carried out experimental crosses between resistant (R) and susceptible (S) strains captured in Argentina during 2005. The R strain was collected from sprayed houses in the north of the province of Salta while the S strain was collected in the province of Chaco. Both strains were bred in the laboratory for reciprocal crosses (F1), intercrosses (F2) and backcrosses (BC). The descendents were tested by a standard insecticide resistance bioassay. Resistance ratios were 1 for S strain, 103.36 for R strain and 18.34 for F1. The regression lines of F1 generations (R. × S and S. × R) showed no significant differences and were closer to that of the R parents, indicating that inheritance of deltamethrin resistance in T. infestans is autosomal and incompletely dominant (D=0.20). Chi-square analysis from responses of intercross and backcross progenies rejected the hypothesis of a single gene being responsible for resistance. The minimum number of independent segregation genes was three, as calculated with Lande's method. The genetic basis here described for the resistant phenotype indicate that, under pyrethroid selective pressure, the resistant genotypes could be easily spread to susceptible insects from resistant individuals, posing a major threat to vectorial control of Chagas disease.
Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; Argentina
Fil: Panzera, Francisco. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Segura, Maria Asuncion. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Pérez, Ruben. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Diaz, Renee Analia. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; Argentina
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Materia
Chagas Disease
Deltametrin
Inheritance of Resistance
Pyrethroids
Triatoma Infestans
Vectorial Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77010
Acceder
id CONICETDig_0a40c61d251b1fd0bbc93949a57558ac
oai_identifier_str oai:ri.conicet.gov.ar:11336/77010
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South AmericaCardozo, Rubén MarinoPanzera, FranciscoGentile, Alberto GerónimoSegura, Maria AsuncionPérez, RubenDiaz, Renee AnaliaBasombrío, Miguel Ángel ManuelChagas DiseaseDeltametrinInheritance of ResistancePyrethroidsTriatoma InfestansVectorial Controlhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An outbreak of pyrethroid resistance was recently detected in Triatoma infestans from northern Argentina. To analyze the inheritance of the resistant phenotype, we carried out experimental crosses between resistant (R) and susceptible (S) strains captured in Argentina during 2005. The R strain was collected from sprayed houses in the north of the province of Salta while the S strain was collected in the province of Chaco. Both strains were bred in the laboratory for reciprocal crosses (F1), intercrosses (F2) and backcrosses (BC). The descendents were tested by a standard insecticide resistance bioassay. Resistance ratios were 1 for S strain, 103.36 for R strain and 18.34 for F1. The regression lines of F1 generations (R. × S and S. × R) showed no significant differences and were closer to that of the R parents, indicating that inheritance of deltamethrin resistance in T. infestans is autosomal and incompletely dominant (D=0.20). Chi-square analysis from responses of intercross and backcross progenies rejected the hypothesis of a single gene being responsible for resistance. The minimum number of independent segregation genes was three, as calculated with Lande's method. The genetic basis here described for the resistant phenotype indicate that, under pyrethroid selective pressure, the resistant genotypes could be easily spread to susceptible insects from resistant individuals, posing a major threat to vectorial control of Chagas disease.Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; ArgentinaFil: Panzera, Francisco. Universidad de la República. Facultad de Ciencias; UruguayFil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; ArgentinaFil: Segura, Maria Asuncion. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Pérez, Ruben. Universidad de la República. Facultad de Ciencias; UruguayFil: Diaz, Renee Analia. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; ArgentinaFil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaElsevier Science2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77010Cardozo, Rubén Marino; Panzera, Francisco; Gentile, Alberto Gerónimo; Segura, Maria Asuncion; Pérez, Ruben; et al.; Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America; Elsevier Science; Infection, Genetics and Evolution; 10; 8; 12-2010; 1174-11781567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134810002054info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2010.07.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:36Zoai:ri.conicet.gov.ar:11336/77010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:36.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
title Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
spellingShingle Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
Cardozo, Rubén Marino
Chagas Disease
Deltametrin
Inheritance of Resistance
Pyrethroids
Triatoma Infestans
Vectorial Control
title_short Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
title_full Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
title_fullStr Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
title_full_unstemmed Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
title_sort Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America
dc.creator.none.fl_str_mv Cardozo, Rubén Marino
Panzera, Francisco
Gentile, Alberto Gerónimo
Segura, Maria Asuncion
Pérez, Ruben
Diaz, Renee Analia
Basombrío, Miguel Ángel Manuel
author Cardozo, Rubén Marino
author_facet Cardozo, Rubén Marino
Panzera, Francisco
Gentile, Alberto Gerónimo
Segura, Maria Asuncion
Pérez, Ruben
Diaz, Renee Analia
Basombrío, Miguel Ángel Manuel
author_role author
author2 Panzera, Francisco
Gentile, Alberto Gerónimo
Segura, Maria Asuncion
Pérez, Ruben
Diaz, Renee Analia
Basombrío, Miguel Ángel Manuel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Deltametrin
Inheritance of Resistance
Pyrethroids
Triatoma Infestans
Vectorial Control
topic Chagas Disease
Deltametrin
Inheritance of Resistance
Pyrethroids
Triatoma Infestans
Vectorial Control
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An outbreak of pyrethroid resistance was recently detected in Triatoma infestans from northern Argentina. To analyze the inheritance of the resistant phenotype, we carried out experimental crosses between resistant (R) and susceptible (S) strains captured in Argentina during 2005. The R strain was collected from sprayed houses in the north of the province of Salta while the S strain was collected in the province of Chaco. Both strains were bred in the laboratory for reciprocal crosses (F1), intercrosses (F2) and backcrosses (BC). The descendents were tested by a standard insecticide resistance bioassay. Resistance ratios were 1 for S strain, 103.36 for R strain and 18.34 for F1. The regression lines of F1 generations (R. × S and S. × R) showed no significant differences and were closer to that of the R parents, indicating that inheritance of deltamethrin resistance in T. infestans is autosomal and incompletely dominant (D=0.20). Chi-square analysis from responses of intercross and backcross progenies rejected the hypothesis of a single gene being responsible for resistance. The minimum number of independent segregation genes was three, as calculated with Lande's method. The genetic basis here described for the resistant phenotype indicate that, under pyrethroid selective pressure, the resistant genotypes could be easily spread to susceptible insects from resistant individuals, posing a major threat to vectorial control of Chagas disease.
Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; Argentina
Fil: Panzera, Francisco. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Segura, Maria Asuncion. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Pérez, Ruben. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Diaz, Renee Analia. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; Argentina
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
description An outbreak of pyrethroid resistance was recently detected in Triatoma infestans from northern Argentina. To analyze the inheritance of the resistant phenotype, we carried out experimental crosses between resistant (R) and susceptible (S) strains captured in Argentina during 2005. The R strain was collected from sprayed houses in the north of the province of Salta while the S strain was collected in the province of Chaco. Both strains were bred in the laboratory for reciprocal crosses (F1), intercrosses (F2) and backcrosses (BC). The descendents were tested by a standard insecticide resistance bioassay. Resistance ratios were 1 for S strain, 103.36 for R strain and 18.34 for F1. The regression lines of F1 generations (R. × S and S. × R) showed no significant differences and were closer to that of the R parents, indicating that inheritance of deltamethrin resistance in T. infestans is autosomal and incompletely dominant (D=0.20). Chi-square analysis from responses of intercross and backcross progenies rejected the hypothesis of a single gene being responsible for resistance. The minimum number of independent segregation genes was three, as calculated with Lande's method. The genetic basis here described for the resistant phenotype indicate that, under pyrethroid selective pressure, the resistant genotypes could be easily spread to susceptible insects from resistant individuals, posing a major threat to vectorial control of Chagas disease.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77010
Cardozo, Rubén Marino; Panzera, Francisco; Gentile, Alberto Gerónimo; Segura, Maria Asuncion; Pérez, Ruben; et al.; Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America; Elsevier Science; Infection, Genetics and Evolution; 10; 8; 12-2010; 1174-1178
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77010
identifier_str_mv Cardozo, Rubén Marino; Panzera, Francisco; Gentile, Alberto Gerónimo; Segura, Maria Asuncion; Pérez, Ruben; et al.; Inheritance of resistance to pyrethroids in Triatoma infestans, the main Chagas disease vector in South America; Elsevier Science; Infection, Genetics and Evolution; 10; 8; 12-2010; 1174-1178
1567-1348
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134810002054
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2010.07.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269810090573824
score 13.13397

Publicaciones similares