Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles

Autores
Ferronato, María Julia; Facchinetti, Maria Marta; Curino, Alejandro Carlos; Agotegaray, Mariela Alejandra; Lasalle, María de Las Nieves
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
INTRODUCTION. Magnetic nanoparticles (MNPs) represent a tool for localized therapeutics on specific sites of the body by the influence of an external magnetic field. They may act as drug carriers as well as contrast agents for resonance magnetic images and agents for hyperthermia treatment in different oncological pathologies. In this work, we developed MNPs coated with different agents in order to induce biocompatible platforms for future loading of chemotherapies. Their effects on cell viability of murine triple negative breast cancer cells (TNBC) were studied to provide novel information for possible future clinical applications.MATERIALS AND METHODS: MNPs were synthesized by co-precipitation method from iron precursors and different coating agents: citric acid, glutamic acid, adenine, oleic acid, beta-cyclodextrin, and 3-aminopropyltriethoxysilane (APTES). Physicochemical characterization was performed employing FTIR spectroscopy, analysis of hydrodynamic diameter and zeta potential, transmission electronic microscopy and iron content. Cell viability of murine 4T1TNBC cells exposed for 24 h to different concentrations of MNPs (0-500 μg/mL) was evaluated by crystal violet staining assays. The experiments were repeated twice and performed in triplicate.RESULTS. Spectroscopic studies demonstrated the successful coating of the different MNPs with the coating agents employed. Physicochemical properties of MNPs were proper for biomedical applications in terms of hydrodynamic diameters (between 200 and 350 nm) and stability in physiological media pH near 7 (PDI<0.5). No differences were found on TNBC cell viability between nano-formulations or control cells at any of the concentrations tested. CONCLUSION. The MNPs studied with different biocompatible coating agents did not alter viability of murine TNBC cells by themselves. In this way, these MNPs may be used as platform for loading of different drugs intended to improve breast cancer current therapies.
Fil: Ferronato, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lasalle, María de Las Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
1st zooming into preclinical Nanomedicines in the era Covid-19
Argentina
La Asociación Argentina de Nanomedicinas
Materia
MAGNETIC
NANOPARTICLES
BREAST CANCER
TRIPLE NEGATIVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199925
Acceder
id CONICETDig_09d83316469eb48c68efec4c50c19006
oai_identifier_str oai:ri.conicet.gov.ar:11336/199925
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticlesFerronato, María JuliaFacchinetti, Maria MartaCurino, Alejandro CarlosAgotegaray, Mariela AlejandraLasalle, María de Las NievesMAGNETICNANOPARTICLESBREAST CANCERTRIPLE NEGATIVEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3INTRODUCTION. Magnetic nanoparticles (MNPs) represent a tool for localized therapeutics on specific sites of the body by the influence of an external magnetic field. They may act as drug carriers as well as contrast agents for resonance magnetic images and agents for hyperthermia treatment in different oncological pathologies. In this work, we developed MNPs coated with different agents in order to induce biocompatible platforms for future loading of chemotherapies. Their effects on cell viability of murine triple negative breast cancer cells (TNBC) were studied to provide novel information for possible future clinical applications.MATERIALS AND METHODS: MNPs were synthesized by co-precipitation method from iron precursors and different coating agents: citric acid, glutamic acid, adenine, oleic acid, beta-cyclodextrin, and 3-aminopropyltriethoxysilane (APTES). Physicochemical characterization was performed employing FTIR spectroscopy, analysis of hydrodynamic diameter and zeta potential, transmission electronic microscopy and iron content. Cell viability of murine 4T1TNBC cells exposed for 24 h to different concentrations of MNPs (0-500 μg/mL) was evaluated by crystal violet staining assays. The experiments were repeated twice and performed in triplicate.RESULTS. Spectroscopic studies demonstrated the successful coating of the different MNPs with the coating agents employed. Physicochemical properties of MNPs were proper for biomedical applications in terms of hydrodynamic diameters (between 200 and 350 nm) and stability in physiological media pH near 7 (PDI<0.5). No differences were found on TNBC cell viability between nano-formulations or control cells at any of the concentrations tested. CONCLUSION. The MNPs studied with different biocompatible coating agents did not alter viability of murine TNBC cells by themselves. In this way, these MNPs may be used as platform for loading of different drugs intended to improve breast cancer current therapies.Fil: Ferronato, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Lasalle, María de Las Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina1st zooming into preclinical Nanomedicines in the era Covid-19ArgentinaLa Asociación Argentina de NanomedicinasAsociación Argentina de Nanomedicinas, NanomedAR2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199925Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles; 1st zooming into preclinical Nanomedicines in the era Covid-19; Argentina; 2020; 26-272718-7098CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:37Zoai:ri.conicet.gov.ar:11336/199925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:37.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
title Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
spellingShingle Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
Ferronato, María Julia
MAGNETIC
NANOPARTICLES
BREAST CANCER
TRIPLE NEGATIVE
title_short Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
title_full Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
title_fullStr Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
title_full_unstemmed Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
title_sort Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles
dc.creator.none.fl_str_mv Ferronato, María Julia
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Agotegaray, Mariela Alejandra
Lasalle, María de Las Nieves
author Ferronato, María Julia
author_facet Ferronato, María Julia
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Agotegaray, Mariela Alejandra
Lasalle, María de Las Nieves
author_role author
author2 Facchinetti, Maria Marta
Curino, Alejandro Carlos
Agotegaray, Mariela Alejandra
Lasalle, María de Las Nieves
author2_role author
author
author
author
dc.subject.none.fl_str_mv MAGNETIC
NANOPARTICLES
BREAST CANCER
TRIPLE NEGATIVE
topic MAGNETIC
NANOPARTICLES
BREAST CANCER
TRIPLE NEGATIVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv INTRODUCTION. Magnetic nanoparticles (MNPs) represent a tool for localized therapeutics on specific sites of the body by the influence of an external magnetic field. They may act as drug carriers as well as contrast agents for resonance magnetic images and agents for hyperthermia treatment in different oncological pathologies. In this work, we developed MNPs coated with different agents in order to induce biocompatible platforms for future loading of chemotherapies. Their effects on cell viability of murine triple negative breast cancer cells (TNBC) were studied to provide novel information for possible future clinical applications.MATERIALS AND METHODS: MNPs were synthesized by co-precipitation method from iron precursors and different coating agents: citric acid, glutamic acid, adenine, oleic acid, beta-cyclodextrin, and 3-aminopropyltriethoxysilane (APTES). Physicochemical characterization was performed employing FTIR spectroscopy, analysis of hydrodynamic diameter and zeta potential, transmission electronic microscopy and iron content. Cell viability of murine 4T1TNBC cells exposed for 24 h to different concentrations of MNPs (0-500 μg/mL) was evaluated by crystal violet staining assays. The experiments were repeated twice and performed in triplicate.RESULTS. Spectroscopic studies demonstrated the successful coating of the different MNPs with the coating agents employed. Physicochemical properties of MNPs were proper for biomedical applications in terms of hydrodynamic diameters (between 200 and 350 nm) and stability in physiological media pH near 7 (PDI<0.5). No differences were found on TNBC cell viability between nano-formulations or control cells at any of the concentrations tested. CONCLUSION. The MNPs studied with different biocompatible coating agents did not alter viability of murine TNBC cells by themselves. In this way, these MNPs may be used as platform for loading of different drugs intended to improve breast cancer current therapies.
Fil: Ferronato, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lasalle, María de Las Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
1st zooming into preclinical Nanomedicines in the era Covid-19
Argentina
La Asociación Argentina de Nanomedicinas
description INTRODUCTION. Magnetic nanoparticles (MNPs) represent a tool for localized therapeutics on specific sites of the body by the influence of an external magnetic field. They may act as drug carriers as well as contrast agents for resonance magnetic images and agents for hyperthermia treatment in different oncological pathologies. In this work, we developed MNPs coated with different agents in order to induce biocompatible platforms for future loading of chemotherapies. Their effects on cell viability of murine triple negative breast cancer cells (TNBC) were studied to provide novel information for possible future clinical applications.MATERIALS AND METHODS: MNPs were synthesized by co-precipitation method from iron precursors and different coating agents: citric acid, glutamic acid, adenine, oleic acid, beta-cyclodextrin, and 3-aminopropyltriethoxysilane (APTES). Physicochemical characterization was performed employing FTIR spectroscopy, analysis of hydrodynamic diameter and zeta potential, transmission electronic microscopy and iron content. Cell viability of murine 4T1TNBC cells exposed for 24 h to different concentrations of MNPs (0-500 μg/mL) was evaluated by crystal violet staining assays. The experiments were repeated twice and performed in triplicate.RESULTS. Spectroscopic studies demonstrated the successful coating of the different MNPs with the coating agents employed. Physicochemical properties of MNPs were proper for biomedical applications in terms of hydrodynamic diameters (between 200 and 350 nm) and stability in physiological media pH near 7 (PDI<0.5). No differences were found on TNBC cell viability between nano-formulations or control cells at any of the concentrations tested. CONCLUSION. The MNPs studied with different biocompatible coating agents did not alter viability of murine TNBC cells by themselves. In this way, these MNPs may be used as platform for loading of different drugs intended to improve breast cancer current therapies.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199925
Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles; 1st zooming into preclinical Nanomedicines in the era Covid-19; Argentina; 2020; 26-27
2718-7098
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199925
identifier_str_mv Evaluation of murine triple negative breast cancer cell viability exposed to magnetic nanoparticles; 1st zooming into preclinical Nanomedicines in the era Covid-19; Argentina; 2020; 26-27
2718-7098
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Asociación Argentina de Nanomedicinas, NanomedAR
publisher.none.fl_str_mv Asociación Argentina de Nanomedicinas, NanomedAR
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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