Electrical mapping of microtubular structures by surface potential microscopy
- Autores
- Zhang, Peng; Cantiello, Horacio Fabio
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.
Fil: Zhang, Peng. Harvard Medical School; Estados Unidos
Fil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Harvard Medical School; Estados Unidos - Materia
-
Microtubules
Electrical properties - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/241804
Ver los metadatos del registro completo
id |
CONICETDig_09c597b2e3914017f61820a2694a23e7 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/241804 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Electrical mapping of microtubular structures by surface potential microscopyZhang, PengCantiello, Horacio FabioMicrotubulesElectrical propertieshttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.Fil: Zhang, Peng. Harvard Medical School; Estados UnidosFil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Harvard Medical School; Estados UnidosAmerican Institute of Physics2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241804Zhang, Peng; Cantiello, Horacio Fabio; Electrical mapping of microtubular structures by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 11; 113703; 9-2009; 1-40003-6951CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1063/1.3212147info:eu-repo/semantics/altIdentifier/url/https://pubs.aip.org/aip/apl/article-abstract/95/11/113703/927341/Electrical-mapping-of-microtubular-structures-by?redirectedFrom=fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:31Zoai:ri.conicet.gov.ar:11336/241804instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:31.794CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Electrical mapping of microtubular structures by surface potential microscopy |
title |
Electrical mapping of microtubular structures by surface potential microscopy |
spellingShingle |
Electrical mapping of microtubular structures by surface potential microscopy Zhang, Peng Microtubules Electrical properties |
title_short |
Electrical mapping of microtubular structures by surface potential microscopy |
title_full |
Electrical mapping of microtubular structures by surface potential microscopy |
title_fullStr |
Electrical mapping of microtubular structures by surface potential microscopy |
title_full_unstemmed |
Electrical mapping of microtubular structures by surface potential microscopy |
title_sort |
Electrical mapping of microtubular structures by surface potential microscopy |
dc.creator.none.fl_str_mv |
Zhang, Peng Cantiello, Horacio Fabio |
author |
Zhang, Peng |
author_facet |
Zhang, Peng Cantiello, Horacio Fabio |
author_role |
author |
author2 |
Cantiello, Horacio Fabio |
author2_role |
author |
dc.subject.none.fl_str_mv |
Microtubules Electrical properties |
topic |
Microtubules Electrical properties |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties. Fil: Zhang, Peng. Harvard Medical School; Estados Unidos Fil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Harvard Medical School; Estados Unidos |
description |
Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/241804 Zhang, Peng; Cantiello, Horacio Fabio; Electrical mapping of microtubular structures by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 11; 113703; 9-2009; 1-4 0003-6951 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/241804 |
identifier_str_mv |
Zhang, Peng; Cantiello, Horacio Fabio; Electrical mapping of microtubular structures by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 11; 113703; 9-2009; 1-4 0003-6951 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1063/1.3212147 info:eu-repo/semantics/altIdentifier/url/https://pubs.aip.org/aip/apl/article-abstract/95/11/113703/927341/Electrical-mapping-of-microtubular-structures-by?redirectedFrom=fulltext |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Institute of Physics |
publisher.none.fl_str_mv |
American Institute of Physics |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613830761512960 |
score |
13.070432 |