Electrical mapping of microtubular structures by surface potential microscopy

Autores
Zhang, Peng; Cantiello, Horacio Fabio
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.
Fil: Zhang, Peng. Harvard Medical School; Estados Unidos
Fil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Harvard Medical School; Estados Unidos
Materia
Microtubules
Electrical properties
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241804
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Electrical mapping of microtubular structures by surface potential microscopyZhang, PengCantiello, Horacio FabioMicrotubulesElectrical propertieshttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.Fil: Zhang, Peng. Harvard Medical School; Estados UnidosFil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Harvard Medical School; Estados UnidosAmerican Institute of Physics2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241804Zhang, Peng; Cantiello, Horacio Fabio; Electrical mapping of microtubular structures by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 11; 113703; 9-2009; 1-40003-6951CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1063/1.3212147info:eu-repo/semantics/altIdentifier/url/https://pubs.aip.org/aip/apl/article-abstract/95/11/113703/927341/Electrical-mapping-of-microtubular-structures-by?redirectedFrom=fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:31:59Zoai:ri.conicet.gov.ar:11336/241804instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:59.662CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrical mapping of microtubular structures by surface potential microscopy
title Electrical mapping of microtubular structures by surface potential microscopy
spellingShingle Electrical mapping of microtubular structures by surface potential microscopy
Zhang, Peng
Microtubules
Electrical properties
title_short Electrical mapping of microtubular structures by surface potential microscopy
title_full Electrical mapping of microtubular structures by surface potential microscopy
title_fullStr Electrical mapping of microtubular structures by surface potential microscopy
title_full_unstemmed Electrical mapping of microtubular structures by surface potential microscopy
title_sort Electrical mapping of microtubular structures by surface potential microscopy
dc.creator.none.fl_str_mv Zhang, Peng
Cantiello, Horacio Fabio
author Zhang, Peng
author_facet Zhang, Peng
Cantiello, Horacio Fabio
author_role author
author2 Cantiello, Horacio Fabio
author2_role author
dc.subject.none.fl_str_mv Microtubules
Electrical properties
topic Microtubules
Electrical properties
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.
Fil: Zhang, Peng. Harvard Medical School; Estados Unidos
Fil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Harvard Medical School; Estados Unidos
description Microtubules sMTsd are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of ab-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy sAFMd, and determined their electrical mapping with surface potential microscopy sSPMd. We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.
publishDate 2009
dc.date.none.fl_str_mv 2009-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241804
Zhang, Peng; Cantiello, Horacio Fabio; Electrical mapping of microtubular structures by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 11; 113703; 9-2009; 1-4
0003-6951
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241804
identifier_str_mv Zhang, Peng; Cantiello, Horacio Fabio; Electrical mapping of microtubular structures by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 11; 113703; 9-2009; 1-4
0003-6951
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1063/1.3212147
info:eu-repo/semantics/altIdentifier/url/https://pubs.aip.org/aip/apl/article-abstract/95/11/113703/927341/Electrical-mapping-of-microtubular-structures-by?redirectedFrom=fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Institute of Physics
publisher.none.fl_str_mv American Institute of Physics
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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