Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism

Autores
Tossi, Vanesa Eleonora; Tossi, Vanesa Eleonora; Lamattina, Lorenzo; Lamattina, Lorenzo; Jenkins, Gareth; Jenkins, Gareth; Cassia, Raul Oscar; Cassia, Raul Oscar
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.
UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.
Fil: Tossi, Vanesa Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Tossi, Vanesa Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Jenkins, Gareth. University of Glasgow; Reino Unido
Fil: Jenkins, Gareth. University of Glasgow; Reino Unido
Fil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
Photomorphogenic1
Photomorphogenic1
Hy5 Homolog
Hy5 Homolog
Nitric Oxide
Nitric Oxide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34394
Acceder
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spelling Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent MechanismUltraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent MechanismTossi, Vanesa EleonoraTossi, Vanesa EleonoraLamattina, LorenzoLamattina, LorenzoJenkins, GarethJenkins, GarethCassia, Raul OscarCassia, Raul OscarPhotomorphogenic1Photomorphogenic1Hy5 HomologHy5 HomologNitric OxideNitric Oxidehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.Fil: Tossi, Vanesa Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Tossi, Vanesa Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Jenkins, Gareth. University of Glasgow; Reino UnidoFil: Jenkins, Gareth. University of Glasgow; Reino UnidoFil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaAmerican Society of Plant BiologistAmerican Society of Plant Biologist2014-022014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34394Tossi, Vanesa Eleonora; Lamattina, Lorenzo; Jenkins, Gareth; Cassia, Raul Oscar; Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism; American Society of Plant Biologist; Plant Physiology; 164; 4; 2-2014; 2220-2230Tossi, Vanesa Eleonora; Lamattina, Lorenzo; Jenkins, Gareth; Cassia, Raul Oscar; Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism; American Society of Plant Biologist; Plant Physiology; 164; 4; 2-2014; 2220-22300032-08890032-0889CONICET DigitalCONICETengenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.113.231753info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.113.231753info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/4/2220info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/4/2220info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:15Zoai:ri.conicet.gov.ar:11336/34394instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:15.485CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
title Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
spellingShingle Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
Tossi, Vanesa Eleonora
Photomorphogenic1
Photomorphogenic1
Hy5 Homolog
Hy5 Homolog
Nitric Oxide
Nitric Oxide
title_short Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
title_full Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
title_fullStr Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
title_full_unstemmed Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
title_sort Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism
dc.creator.none.fl_str_mv Tossi, Vanesa Eleonora
Tossi, Vanesa Eleonora
Lamattina, Lorenzo
Lamattina, Lorenzo
Jenkins, Gareth
Jenkins, Gareth
Cassia, Raul Oscar
Cassia, Raul Oscar
author Tossi, Vanesa Eleonora
author_facet Tossi, Vanesa Eleonora
Lamattina, Lorenzo
Jenkins, Gareth
Cassia, Raul Oscar
author_role author
author2 Lamattina, Lorenzo
Jenkins, Gareth
Cassia, Raul Oscar
author2_role author
author
author
dc.subject.none.fl_str_mv Photomorphogenic1
Photomorphogenic1
Hy5 Homolog
Hy5 Homolog
Nitric Oxide
Nitric Oxide
topic Photomorphogenic1
Photomorphogenic1
Hy5 Homolog
Hy5 Homolog
Nitric Oxide
Nitric Oxide
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.
UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.
Fil: Tossi, Vanesa Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Tossi, Vanesa Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Jenkins, Gareth. University of Glasgow; Reino Unido
Fil: Jenkins, Gareth. University of Glasgow; Reino Unido
Fil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATEDHYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated byUVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. Inthis study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatalmovements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsbergerectaclosed in response to increasing UV-Bfluence rates, with maximal closure after 3-h exposure to 5.46mmol m–2s–1UV-B.Both hydrogen peroxide (H2O2) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, didnot close in response to UV-B, although H2O2still increased. When theuvr8-1null mutant was exposed to UV-B, stomata remainedopen, irrespective of thefluence rate. Neither NO nor H2O2increased in stomata of theuvr8-1mutant. However, the NO donorS-nitrosoglutathione induced closure ofuvr8-1stomata to the same extent as in the wild type. Experiments with mutants in UVR8signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-inducedstomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involvingboth H2O2and NO generation in response to UV-B exposure.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34394
Tossi, Vanesa Eleonora; Lamattina, Lorenzo; Jenkins, Gareth; Cassia, Raul Oscar; Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism; American Society of Plant Biologist; Plant Physiology; 164; 4; 2-2014; 2220-2230
Tossi, Vanesa Eleonora; Lamattina, Lorenzo; Jenkins, Gareth; Cassia, Raul Oscar; Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism; American Society of Plant Biologist; Plant Physiology; 164; 4; 2-2014; 2220-2230
0032-0889
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34394
identifier_str_mv Tossi, Vanesa Eleonora; Lamattina, Lorenzo; Jenkins, Gareth; Cassia, Raul Oscar; Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism; American Society of Plant Biologist; Plant Physiology; 164; 4; 2-2014; 2220-2230
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.113.231753
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info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/4/2220
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/4/2220
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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