What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach

Autores
Ontañon, Ornella Mailén; Landi, Claudia; Carleo, Alfonso; Gagliardi, Assunta; Bianchi, Laura; González, Paola Solange; Agostini, Elizabeth; Bini, Luca
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acinetobacter guillouiae SFC 500-1A is an environmental bacterium able to efficiently co-remediate phenol and Cr(VI). To further understand the molecular mechanisms triggered in this strain during the bioremediation process, variations in the proteomic profile after treatment with phenol and phenol plus Cr(VI) were evaluated. The proteomic analysis revealed the induction of the β-ketoadipate pathway for phenol oxidation and the assimilation of degradation products through TCA cycle and glyoxylate shunt. Phenol exposure increased the abundance of proteins associated to energetic processes and ATP synthesis, but it also triggered cellular stress. The lipid bilayer was suggested as a target of phenol toxicity, and changing fatty acids composition seemed to be the bacterial response to protect the membrane integrity. The involvement of two flavoproteins in Cr(VI) reduction to Cr(III) was also proposed. The results suggested the important role of chaperones, antioxidant response and SOS-induced proteins in the ability of the strain to mitigate the damage generated by phenol and Cr(VI). This research contributes to elucidate the mechanisms involved in A. guillouiae SFC 500-1A tolerance and co-remediation of phenol and Cr(VI). Such information may result useful not only to improve its bioremediation efficiency but also to identify putative markers of resistance in environmental bacteria.
Fil: Ontañon, Ornella Mailén. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Landi, Claudia. Università degli Studi di Siena; Italia
Fil: Carleo, Alfonso. Hannover Medical School; Alemania. Università degli Studi di Siena; Italia
Fil: Gagliardi, Assunta. University of Trento; Italia. Università degli Studi di Siena; Italia
Fil: Bianchi, Laura. Università degli Studi di Siena; Italia
Fil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bini, Luca. Università degli Studi di Siena; Italia
Materia
ACINETOBACTER
CHROMIUM (VI)
PHENOL
PROTEOMICS
SIMULTANEOUS BIOREMEDIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99542
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99542
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approachOntañon, Ornella MailénLandi, ClaudiaCarleo, AlfonsoGagliardi, AssuntaBianchi, LauraGonzález, Paola SolangeAgostini, ElizabethBini, LucaACINETOBACTERCHROMIUM (VI)PHENOLPROTEOMICSSIMULTANEOUS BIOREMEDIATIONhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Acinetobacter guillouiae SFC 500-1A is an environmental bacterium able to efficiently co-remediate phenol and Cr(VI). To further understand the molecular mechanisms triggered in this strain during the bioremediation process, variations in the proteomic profile after treatment with phenol and phenol plus Cr(VI) were evaluated. The proteomic analysis revealed the induction of the β-ketoadipate pathway for phenol oxidation and the assimilation of degradation products through TCA cycle and glyoxylate shunt. Phenol exposure increased the abundance of proteins associated to energetic processes and ATP synthesis, but it also triggered cellular stress. The lipid bilayer was suggested as a target of phenol toxicity, and changing fatty acids composition seemed to be the bacterial response to protect the membrane integrity. The involvement of two flavoproteins in Cr(VI) reduction to Cr(III) was also proposed. The results suggested the important role of chaperones, antioxidant response and SOS-induced proteins in the ability of the strain to mitigate the damage generated by phenol and Cr(VI). This research contributes to elucidate the mechanisms involved in A. guillouiae SFC 500-1A tolerance and co-remediation of phenol and Cr(VI). Such information may result useful not only to improve its bioremediation efficiency but also to identify putative markers of resistance in environmental bacteria.Fil: Ontañon, Ornella Mailén. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Landi, Claudia. Università degli Studi di Siena; ItaliaFil: Carleo, Alfonso. Hannover Medical School; Alemania. Università degli Studi di Siena; ItaliaFil: Gagliardi, Assunta. University of Trento; Italia. Università degli Studi di Siena; ItaliaFil: Bianchi, Laura. Università degli Studi di Siena; ItaliaFil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bini, Luca. Università degli Studi di Siena; ItaliaElsevier Science2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99542Ontañon, Ornella Mailén; Landi, Claudia; Carleo, Alfonso; Gagliardi, Assunta; Bianchi, Laura; et al.; What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach; Elsevier Science; Journal of Hazardous Materials; 354; 7-2018; 215-2240304-3894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304389418303236info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jhazmat.2018.04.068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:48Zoai:ri.conicet.gov.ar:11336/99542instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:49.191CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
title What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
spellingShingle What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
Ontañon, Ornella Mailén
ACINETOBACTER
CHROMIUM (VI)
PHENOL
PROTEOMICS
SIMULTANEOUS BIOREMEDIATION
title_short What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
title_full What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
title_fullStr What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
title_full_unstemmed What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
title_sort What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
dc.creator.none.fl_str_mv Ontañon, Ornella Mailén
Landi, Claudia
Carleo, Alfonso
Gagliardi, Assunta
Bianchi, Laura
González, Paola Solange
Agostini, Elizabeth
Bini, Luca
author Ontañon, Ornella Mailén
author_facet Ontañon, Ornella Mailén
Landi, Claudia
Carleo, Alfonso
Gagliardi, Assunta
Bianchi, Laura
González, Paola Solange
Agostini, Elizabeth
Bini, Luca
author_role author
author2 Landi, Claudia
Carleo, Alfonso
Gagliardi, Assunta
Bianchi, Laura
González, Paola Solange
Agostini, Elizabeth
Bini, Luca
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER
CHROMIUM (VI)
PHENOL
PROTEOMICS
SIMULTANEOUS BIOREMEDIATION
topic ACINETOBACTER
CHROMIUM (VI)
PHENOL
PROTEOMICS
SIMULTANEOUS BIOREMEDIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Acinetobacter guillouiae SFC 500-1A is an environmental bacterium able to efficiently co-remediate phenol and Cr(VI). To further understand the molecular mechanisms triggered in this strain during the bioremediation process, variations in the proteomic profile after treatment with phenol and phenol plus Cr(VI) were evaluated. The proteomic analysis revealed the induction of the β-ketoadipate pathway for phenol oxidation and the assimilation of degradation products through TCA cycle and glyoxylate shunt. Phenol exposure increased the abundance of proteins associated to energetic processes and ATP synthesis, but it also triggered cellular stress. The lipid bilayer was suggested as a target of phenol toxicity, and changing fatty acids composition seemed to be the bacterial response to protect the membrane integrity. The involvement of two flavoproteins in Cr(VI) reduction to Cr(III) was also proposed. The results suggested the important role of chaperones, antioxidant response and SOS-induced proteins in the ability of the strain to mitigate the damage generated by phenol and Cr(VI). This research contributes to elucidate the mechanisms involved in A. guillouiae SFC 500-1A tolerance and co-remediation of phenol and Cr(VI). Such information may result useful not only to improve its bioremediation efficiency but also to identify putative markers of resistance in environmental bacteria.
Fil: Ontañon, Ornella Mailén. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Landi, Claudia. Università degli Studi di Siena; Italia
Fil: Carleo, Alfonso. Hannover Medical School; Alemania. Università degli Studi di Siena; Italia
Fil: Gagliardi, Assunta. University of Trento; Italia. Università degli Studi di Siena; Italia
Fil: Bianchi, Laura. Università degli Studi di Siena; Italia
Fil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bini, Luca. Università degli Studi di Siena; Italia
description Acinetobacter guillouiae SFC 500-1A is an environmental bacterium able to efficiently co-remediate phenol and Cr(VI). To further understand the molecular mechanisms triggered in this strain during the bioremediation process, variations in the proteomic profile after treatment with phenol and phenol plus Cr(VI) were evaluated. The proteomic analysis revealed the induction of the β-ketoadipate pathway for phenol oxidation and the assimilation of degradation products through TCA cycle and glyoxylate shunt. Phenol exposure increased the abundance of proteins associated to energetic processes and ATP synthesis, but it also triggered cellular stress. The lipid bilayer was suggested as a target of phenol toxicity, and changing fatty acids composition seemed to be the bacterial response to protect the membrane integrity. The involvement of two flavoproteins in Cr(VI) reduction to Cr(III) was also proposed. The results suggested the important role of chaperones, antioxidant response and SOS-induced proteins in the ability of the strain to mitigate the damage generated by phenol and Cr(VI). This research contributes to elucidate the mechanisms involved in A. guillouiae SFC 500-1A tolerance and co-remediation of phenol and Cr(VI). Such information may result useful not only to improve its bioremediation efficiency but also to identify putative markers of resistance in environmental bacteria.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99542
Ontañon, Ornella Mailén; Landi, Claudia; Carleo, Alfonso; Gagliardi, Assunta; Bianchi, Laura; et al.; What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach; Elsevier Science; Journal of Hazardous Materials; 354; 7-2018; 215-224
0304-3894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99542
identifier_str_mv Ontañon, Ornella Mailén; Landi, Claudia; Carleo, Alfonso; Gagliardi, Assunta; Bianchi, Laura; et al.; What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach; Elsevier Science; Journal of Hazardous Materials; 354; 7-2018; 215-224
0304-3894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304389418303236
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jhazmat.2018.04.068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269660779642880
score 13.13397

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