Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan

Autores
Bocanegra, María Victoria; Manucha, Walter Ariel Fernando; Rodriguez Peña, Marcelo Javier; Cacciamani, Valeria; Vallés, Patricia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Caveolin is required to traffic the AT1 receptor through the exocytic pathway. The chaperone Hsp70 regulates a diverse set of signaling pathways via their interactions with proteins. Here we examined the AT1 receptor antagonist Losartan effect on Caveolin-1 and Hsp70 protein association in SHR proximal tubules (PT). Hsp70 involvement on Losartan oxidative stress regulation was also studied. Four-week-old SHR were randomized for receiving Losartan (40 mg.kg-1day) (SHRLos) or no treatment (SHRH2O) during 8 weeks. Wistar-Kyoto rats (WKY) were normotensive controls. By western blotting, the relative abundance of Caveolin-1 was 2-fold higher in microdissected PT membrane fractions from treated SHR vs WKYH2O. Hsp70 membrane translocation was demonstrated in SHRLos through out the upregulation of Hsp70 expression in microdissected PT membrane fractions when compared with WKYH2O, p<0.001. Conversely, decreased Hsp70 protein levels were shown in microdissected PT cytosol fraction from SHRLos, p<0.01. Interaction between Cav-1 and Hsp70 was further determined by coimmunoprecipitation and by immunofluorescence colocalization in SHRLos PT membranes. After membrane translocation of Hsp70, the decreased NADPH oxidase activity (RFU/ìprot/min incubation) near controls demonstrated on microdissected PT membranes from SHRLos vs. SHRH2O, p<0.01, was reversed by the pre-incubation with anti-Hsp70 antibody. In addition, interaction between Hsp70 and Nox4 was determined by the coimmunoprecipitation strategy showing that membrane overexpression of Hsp70 was associated with decreased Nox4 after Losartan treatment in SHR. Conclusion: After Losartan administration interaction of Caveolin-1 and Hsp70 was shown in SHR proximal tubules. Translocation of Hsp70 to PT membranes in SHRLos might exert a cytoprotective effect by downregulation of NADPH isoform Nox4.
Fil: Bocanegra, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Rodriguez Peña, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cacciamani, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Vallés, Patricia. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Materia
CAVEOLIN-1
HSP70
SHR
LOSARTAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276941
Acceder
id CONICETDig_06dc3e50e85d9956eb39b4d641d05dd6
oai_identifier_str oai:ri.conicet.gov.ar:11336/276941
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of LosartanBocanegra, María VictoriaManucha, Walter Ariel FernandoRodriguez Peña, Marcelo JavierCacciamani, ValeriaVallés, PatriciaCAVEOLIN-1HSP70SHRLOSARTANhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Caveolin is required to traffic the AT1 receptor through the exocytic pathway. The chaperone Hsp70 regulates a diverse set of signaling pathways via their interactions with proteins. Here we examined the AT1 receptor antagonist Losartan effect on Caveolin-1 and Hsp70 protein association in SHR proximal tubules (PT). Hsp70 involvement on Losartan oxidative stress regulation was also studied. Four-week-old SHR were randomized for receiving Losartan (40 mg.kg-1day) (SHRLos) or no treatment (SHRH2O) during 8 weeks. Wistar-Kyoto rats (WKY) were normotensive controls. By western blotting, the relative abundance of Caveolin-1 was 2-fold higher in microdissected PT membrane fractions from treated SHR vs WKYH2O. Hsp70 membrane translocation was demonstrated in SHRLos through out the upregulation of Hsp70 expression in microdissected PT membrane fractions when compared with WKYH2O, p<0.001. Conversely, decreased Hsp70 protein levels were shown in microdissected PT cytosol fraction from SHRLos, p<0.01. Interaction between Cav-1 and Hsp70 was further determined by coimmunoprecipitation and by immunofluorescence colocalization in SHRLos PT membranes. After membrane translocation of Hsp70, the decreased NADPH oxidase activity (RFU/ìprot/min incubation) near controls demonstrated on microdissected PT membranes from SHRLos vs. SHRH2O, p<0.01, was reversed by the pre-incubation with anti-Hsp70 antibody. In addition, interaction between Hsp70 and Nox4 was determined by the coimmunoprecipitation strategy showing that membrane overexpression of Hsp70 was associated with decreased Nox4 after Losartan treatment in SHR. Conclusion: After Losartan administration interaction of Caveolin-1 and Hsp70 was shown in SHR proximal tubules. Translocation of Hsp70 to PT membranes in SHRLos might exert a cytoprotective effect by downregulation of NADPH isoform Nox4.Fil: Bocanegra, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; ArgentinaFil: Rodriguez Peña, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cacciamani, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; ArgentinaFil: Vallés, Patricia. Universidad del Aconcagua. Facultad de Ciencias Médicas; ArgentinaLippincott Williams2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276941Bocanegra, María Victoria; Manucha, Walter Ariel Fernando; Rodriguez Peña, Marcelo Javier; Cacciamani, Valeria; Vallés, Patricia; Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan; Lippincott Williams; Journal of Hypertension; 28; 1; 1-2010; 143-1550263-6352CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1097/HJH.0b013e328332b778info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:17:32Zoai:ri.conicet.gov.ar:11336/276941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:17:32.636CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
title Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
spellingShingle Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
Bocanegra, María Victoria
CAVEOLIN-1
HSP70
SHR
LOSARTAN
title_short Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
title_full Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
title_fullStr Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
title_full_unstemmed Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
title_sort Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
dc.creator.none.fl_str_mv Bocanegra, María Victoria
Manucha, Walter Ariel Fernando
Rodriguez Peña, Marcelo Javier
Cacciamani, Valeria
Vallés, Patricia
author Bocanegra, María Victoria
author_facet Bocanegra, María Victoria
Manucha, Walter Ariel Fernando
Rodriguez Peña, Marcelo Javier
Cacciamani, Valeria
Vallés, Patricia
author_role author
author2 Manucha, Walter Ariel Fernando
Rodriguez Peña, Marcelo Javier
Cacciamani, Valeria
Vallés, Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv CAVEOLIN-1
HSP70
SHR
LOSARTAN
topic CAVEOLIN-1
HSP70
SHR
LOSARTAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Caveolin is required to traffic the AT1 receptor through the exocytic pathway. The chaperone Hsp70 regulates a diverse set of signaling pathways via their interactions with proteins. Here we examined the AT1 receptor antagonist Losartan effect on Caveolin-1 and Hsp70 protein association in SHR proximal tubules (PT). Hsp70 involvement on Losartan oxidative stress regulation was also studied. Four-week-old SHR were randomized for receiving Losartan (40 mg.kg-1day) (SHRLos) or no treatment (SHRH2O) during 8 weeks. Wistar-Kyoto rats (WKY) were normotensive controls. By western blotting, the relative abundance of Caveolin-1 was 2-fold higher in microdissected PT membrane fractions from treated SHR vs WKYH2O. Hsp70 membrane translocation was demonstrated in SHRLos through out the upregulation of Hsp70 expression in microdissected PT membrane fractions when compared with WKYH2O, p<0.001. Conversely, decreased Hsp70 protein levels were shown in microdissected PT cytosol fraction from SHRLos, p<0.01. Interaction between Cav-1 and Hsp70 was further determined by coimmunoprecipitation and by immunofluorescence colocalization in SHRLos PT membranes. After membrane translocation of Hsp70, the decreased NADPH oxidase activity (RFU/ìprot/min incubation) near controls demonstrated on microdissected PT membranes from SHRLos vs. SHRH2O, p<0.01, was reversed by the pre-incubation with anti-Hsp70 antibody. In addition, interaction between Hsp70 and Nox4 was determined by the coimmunoprecipitation strategy showing that membrane overexpression of Hsp70 was associated with decreased Nox4 after Losartan treatment in SHR. Conclusion: After Losartan administration interaction of Caveolin-1 and Hsp70 was shown in SHR proximal tubules. Translocation of Hsp70 to PT membranes in SHRLos might exert a cytoprotective effect by downregulation of NADPH isoform Nox4.
Fil: Bocanegra, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Rodriguez Peña, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cacciamani, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Vallés, Patricia. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
description Caveolin is required to traffic the AT1 receptor through the exocytic pathway. The chaperone Hsp70 regulates a diverse set of signaling pathways via their interactions with proteins. Here we examined the AT1 receptor antagonist Losartan effect on Caveolin-1 and Hsp70 protein association in SHR proximal tubules (PT). Hsp70 involvement on Losartan oxidative stress regulation was also studied. Four-week-old SHR were randomized for receiving Losartan (40 mg.kg-1day) (SHRLos) or no treatment (SHRH2O) during 8 weeks. Wistar-Kyoto rats (WKY) were normotensive controls. By western blotting, the relative abundance of Caveolin-1 was 2-fold higher in microdissected PT membrane fractions from treated SHR vs WKYH2O. Hsp70 membrane translocation was demonstrated in SHRLos through out the upregulation of Hsp70 expression in microdissected PT membrane fractions when compared with WKYH2O, p<0.001. Conversely, decreased Hsp70 protein levels were shown in microdissected PT cytosol fraction from SHRLos, p<0.01. Interaction between Cav-1 and Hsp70 was further determined by coimmunoprecipitation and by immunofluorescence colocalization in SHRLos PT membranes. After membrane translocation of Hsp70, the decreased NADPH oxidase activity (RFU/ìprot/min incubation) near controls demonstrated on microdissected PT membranes from SHRLos vs. SHRH2O, p<0.01, was reversed by the pre-incubation with anti-Hsp70 antibody. In addition, interaction between Hsp70 and Nox4 was determined by the coimmunoprecipitation strategy showing that membrane overexpression of Hsp70 was associated with decreased Nox4 after Losartan treatment in SHR. Conclusion: After Losartan administration interaction of Caveolin-1 and Hsp70 was shown in SHR proximal tubules. Translocation of Hsp70 to PT membranes in SHRLos might exert a cytoprotective effect by downregulation of NADPH isoform Nox4.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276941
Bocanegra, María Victoria; Manucha, Walter Ariel Fernando; Rodriguez Peña, Marcelo Javier; Cacciamani, Valeria; Vallés, Patricia; Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan; Lippincott Williams; Journal of Hypertension; 28; 1; 1-2010; 143-155
0263-6352
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276941
identifier_str_mv Bocanegra, María Victoria; Manucha, Walter Ariel Fernando; Rodriguez Peña, Marcelo Javier; Cacciamani, Valeria; Vallés, Patricia; Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan; Lippincott Williams; Journal of Hypertension; 28; 1; 1-2010; 143-155
0263-6352
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1097/HJH.0b013e328332b778
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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