A viral DNA-binding domain elicits anti-DNA antibodies of different specificities

Autores
Cerutti, Maria Laura; Zarebski, Laura Mariana; de Prat Gay, Gonzalo; Goldbaum, Fernando Alberto
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously demonstrated that immunization of normal mice with a defined protein:DNA complex comprising the C-terminal DNA-binding domain of the human papillomavirus E2 protein and its cognate site 35 oligonucleotide, results in high antibody titers against both E2 and its target DNA sequence. Here we show that repeated immunization with the isolated form of the E2 domain also elicits anti-DNA antibodies, but in this case, no preferential binding for a given sequence was observed, indicating that these antibodies have broad specificity for DNA. Taken together our results indicate that this viral protein can induce two classes of anti-DNA antibody responses: one directed against endogenous DNA and other in which anti-site 35-specific antibodies are produced. In both cases, the character of the resulting anti-DNA response seems to be directed by the DNA molecule that the protein binds in vivo. Evaluation of the fine specificity of the antibodies induced by the free and bound states of this single foreign DNA-binding protein would contribute to the understanding of the processes involved in the acquisition of particular DNA specificities by anti-DNA antibodies.
Fil: Cerutti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zarebski, Laura Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: de Prat Gay, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Anti-Dna Antibodies
Autoantibodies
Human Papillomavirus (Hpv)
Transcription Factors/Gene Regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39254
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A viral DNA-binding domain elicits anti-DNA antibodies of different specificitiesCerutti, Maria LauraZarebski, Laura Marianade Prat Gay, GonzaloGoldbaum, Fernando AlbertoAnti-Dna AntibodiesAutoantibodiesHuman Papillomavirus (Hpv)Transcription Factors/Gene Regulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have previously demonstrated that immunization of normal mice with a defined protein:DNA complex comprising the C-terminal DNA-binding domain of the human papillomavirus E2 protein and its cognate site 35 oligonucleotide, results in high antibody titers against both E2 and its target DNA sequence. Here we show that repeated immunization with the isolated form of the E2 domain also elicits anti-DNA antibodies, but in this case, no preferential binding for a given sequence was observed, indicating that these antibodies have broad specificity for DNA. Taken together our results indicate that this viral protein can induce two classes of anti-DNA antibody responses: one directed against endogenous DNA and other in which anti-site 35-specific antibodies are produced. In both cases, the character of the resulting anti-DNA response seems to be directed by the DNA molecule that the protein binds in vivo. Evaluation of the fine specificity of the antibodies induced by the free and bound states of this single foreign DNA-binding protein would contribute to the understanding of the processes involved in the acquisition of particular DNA specificities by anti-DNA antibodies.Fil: Cerutti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Zarebski, Laura Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: de Prat Gay, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaPergamon-Elsevier Science Ltd2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39254Cerutti, Maria Laura; Zarebski, Laura Mariana; de Prat Gay, Gonzalo; Goldbaum, Fernando Alberto; A viral DNA-binding domain elicits anti-DNA antibodies of different specificities; Pergamon-Elsevier Science Ltd; Molecular Immunology; 42; 3; 2-2005; 327-3330161-58901872-9142CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(04)00336-0info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2004.09.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:44Zoai:ri.conicet.gov.ar:11336/39254instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:44.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
title A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
spellingShingle A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
Cerutti, Maria Laura
Anti-Dna Antibodies
Autoantibodies
Human Papillomavirus (Hpv)
Transcription Factors/Gene Regulation
title_short A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
title_full A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
title_fullStr A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
title_full_unstemmed A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
title_sort A viral DNA-binding domain elicits anti-DNA antibodies of different specificities
dc.creator.none.fl_str_mv Cerutti, Maria Laura
Zarebski, Laura Mariana
de Prat Gay, Gonzalo
Goldbaum, Fernando Alberto
author Cerutti, Maria Laura
author_facet Cerutti, Maria Laura
Zarebski, Laura Mariana
de Prat Gay, Gonzalo
Goldbaum, Fernando Alberto
author_role author
author2 Zarebski, Laura Mariana
de Prat Gay, Gonzalo
Goldbaum, Fernando Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Anti-Dna Antibodies
Autoantibodies
Human Papillomavirus (Hpv)
Transcription Factors/Gene Regulation
topic Anti-Dna Antibodies
Autoantibodies
Human Papillomavirus (Hpv)
Transcription Factors/Gene Regulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have previously demonstrated that immunization of normal mice with a defined protein:DNA complex comprising the C-terminal DNA-binding domain of the human papillomavirus E2 protein and its cognate site 35 oligonucleotide, results in high antibody titers against both E2 and its target DNA sequence. Here we show that repeated immunization with the isolated form of the E2 domain also elicits anti-DNA antibodies, but in this case, no preferential binding for a given sequence was observed, indicating that these antibodies have broad specificity for DNA. Taken together our results indicate that this viral protein can induce two classes of anti-DNA antibody responses: one directed against endogenous DNA and other in which anti-site 35-specific antibodies are produced. In both cases, the character of the resulting anti-DNA response seems to be directed by the DNA molecule that the protein binds in vivo. Evaluation of the fine specificity of the antibodies induced by the free and bound states of this single foreign DNA-binding protein would contribute to the understanding of the processes involved in the acquisition of particular DNA specificities by anti-DNA antibodies.
Fil: Cerutti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zarebski, Laura Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: de Prat Gay, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description We have previously demonstrated that immunization of normal mice with a defined protein:DNA complex comprising the C-terminal DNA-binding domain of the human papillomavirus E2 protein and its cognate site 35 oligonucleotide, results in high antibody titers against both E2 and its target DNA sequence. Here we show that repeated immunization with the isolated form of the E2 domain also elicits anti-DNA antibodies, but in this case, no preferential binding for a given sequence was observed, indicating that these antibodies have broad specificity for DNA. Taken together our results indicate that this viral protein can induce two classes of anti-DNA antibody responses: one directed against endogenous DNA and other in which anti-site 35-specific antibodies are produced. In both cases, the character of the resulting anti-DNA response seems to be directed by the DNA molecule that the protein binds in vivo. Evaluation of the fine specificity of the antibodies induced by the free and bound states of this single foreign DNA-binding protein would contribute to the understanding of the processes involved in the acquisition of particular DNA specificities by anti-DNA antibodies.
publishDate 2005
dc.date.none.fl_str_mv 2005-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39254
Cerutti, Maria Laura; Zarebski, Laura Mariana; de Prat Gay, Gonzalo; Goldbaum, Fernando Alberto; A viral DNA-binding domain elicits anti-DNA antibodies of different specificities; Pergamon-Elsevier Science Ltd; Molecular Immunology; 42; 3; 2-2005; 327-333
0161-5890
1872-9142
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39254
identifier_str_mv Cerutti, Maria Laura; Zarebski, Laura Mariana; de Prat Gay, Gonzalo; Goldbaum, Fernando Alberto; A viral DNA-binding domain elicits anti-DNA antibodies of different specificities; Pergamon-Elsevier Science Ltd; Molecular Immunology; 42; 3; 2-2005; 327-333
0161-5890
1872-9142
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(04)00336-0
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2004.09.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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