ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells

Autores
Scodelaro Bilbao, Paola Gabriela; Boland, Ricardo Leopoldo; Russo, Ana Josefa; Santillán, Graciela Edith
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the breast tumor cell line MCF-7, extracellular nucleotides induce transient elevations in intracellular calcium concentration ([Ca2+]i). In this study we show that stimulation with ATP or UTP sensitizes MCF-7 cells to mechanical stress leading to an additional transient Ca2+ influx. ATP ⩾ ATPγ-S ⩾ UTP >>> ADP = ADPβ-S elevate [Ca2+]i, proving the presence of P2Y2/P2Y4 purinergic receptor subtypes. In addition, cell stimulation with ATP, ATPγ-S or UTP but not ADPβ-S induced the phosphorylation of ERK1/2, p38 and JNK1/2 mitogen activated protein kinases (MAPKs). The use of Gd3+, La3+ or a Ca2+-free medium, inhibited ATP-dependent stress activated Ca2+ (SAC) influx, but had no effect on MAPK phosphorylation. ATP-induced activation of MAPKs was diminished by two PI-PLC inhibitors and an IP3 receptor antagonist. These results evidence an ATP-sensitive SAC influx in MCF-7 cells and indicate that phosphorylation of MAPKs by ATP is dependent on PI-PLC/IP3/Ca2+i release but independent of SAC influx in these cells, differently to other cell types.
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
Atp
Mapks
Ca2+
Breast Tumor Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75907
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/75907
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cellsScodelaro Bilbao, Paola GabrielaBoland, Ricardo LeopoldoRusso, Ana JosefaSantillán, Graciela EdithAtpMapksCa2+Breast Tumor Cellshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the breast tumor cell line MCF-7, extracellular nucleotides induce transient elevations in intracellular calcium concentration ([Ca2+]i). In this study we show that stimulation with ATP or UTP sensitizes MCF-7 cells to mechanical stress leading to an additional transient Ca2+ influx. ATP ⩾ ATPγ-S ⩾ UTP >>> ADP = ADPβ-S elevate [Ca2+]i, proving the presence of P2Y2/P2Y4 purinergic receptor subtypes. In addition, cell stimulation with ATP, ATPγ-S or UTP but not ADPβ-S induced the phosphorylation of ERK1/2, p38 and JNK1/2 mitogen activated protein kinases (MAPKs). The use of Gd3+, La3+ or a Ca2+-free medium, inhibited ATP-dependent stress activated Ca2+ (SAC) influx, but had no effect on MAPK phosphorylation. ATP-induced activation of MAPKs was diminished by two PI-PLC inhibitors and an IP3 receptor antagonist. These results evidence an ATP-sensitive SAC influx in MCF-7 cells and indicate that phosphorylation of MAPKs by ATP is dependent on PI-PLC/IP3/Ca2+i release but independent of SAC influx in these cells, differently to other cell types.Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaElsevier Science Inc2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75907Scodelaro Bilbao, Paola Gabriela; Boland, Ricardo Leopoldo; Russo, Ana Josefa; Santillán, Graciela Edith; ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 466; 1; 1-10-2007; 15-230003-9861CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003986107003682info:eu-repo/semantics/altIdentifier/doi/10.1016/j.abb.2007.07.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:18Zoai:ri.conicet.gov.ar:11336/75907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:19.217CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
title ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
spellingShingle ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
Scodelaro Bilbao, Paola Gabriela
Atp
Mapks
Ca2+
Breast Tumor Cells
title_short ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
title_full ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
title_fullStr ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
title_full_unstemmed ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
title_sort ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
dc.creator.none.fl_str_mv Scodelaro Bilbao, Paola Gabriela
Boland, Ricardo Leopoldo
Russo, Ana Josefa
Santillán, Graciela Edith
author Scodelaro Bilbao, Paola Gabriela
author_facet Scodelaro Bilbao, Paola Gabriela
Boland, Ricardo Leopoldo
Russo, Ana Josefa
Santillán, Graciela Edith
author_role author
author2 Boland, Ricardo Leopoldo
Russo, Ana Josefa
Santillán, Graciela Edith
author2_role author
author
author
dc.subject.none.fl_str_mv Atp
Mapks
Ca2+
Breast Tumor Cells
topic Atp
Mapks
Ca2+
Breast Tumor Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the breast tumor cell line MCF-7, extracellular nucleotides induce transient elevations in intracellular calcium concentration ([Ca2+]i). In this study we show that stimulation with ATP or UTP sensitizes MCF-7 cells to mechanical stress leading to an additional transient Ca2+ influx. ATP ⩾ ATPγ-S ⩾ UTP >>> ADP = ADPβ-S elevate [Ca2+]i, proving the presence of P2Y2/P2Y4 purinergic receptor subtypes. In addition, cell stimulation with ATP, ATPγ-S or UTP but not ADPβ-S induced the phosphorylation of ERK1/2, p38 and JNK1/2 mitogen activated protein kinases (MAPKs). The use of Gd3+, La3+ or a Ca2+-free medium, inhibited ATP-dependent stress activated Ca2+ (SAC) influx, but had no effect on MAPK phosphorylation. ATP-induced activation of MAPKs was diminished by two PI-PLC inhibitors and an IP3 receptor antagonist. These results evidence an ATP-sensitive SAC influx in MCF-7 cells and indicate that phosphorylation of MAPKs by ATP is dependent on PI-PLC/IP3/Ca2+i release but independent of SAC influx in these cells, differently to other cell types.
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description In the breast tumor cell line MCF-7, extracellular nucleotides induce transient elevations in intracellular calcium concentration ([Ca2+]i). In this study we show that stimulation with ATP or UTP sensitizes MCF-7 cells to mechanical stress leading to an additional transient Ca2+ influx. ATP ⩾ ATPγ-S ⩾ UTP >>> ADP = ADPβ-S elevate [Ca2+]i, proving the presence of P2Y2/P2Y4 purinergic receptor subtypes. In addition, cell stimulation with ATP, ATPγ-S or UTP but not ADPβ-S induced the phosphorylation of ERK1/2, p38 and JNK1/2 mitogen activated protein kinases (MAPKs). The use of Gd3+, La3+ or a Ca2+-free medium, inhibited ATP-dependent stress activated Ca2+ (SAC) influx, but had no effect on MAPK phosphorylation. ATP-induced activation of MAPKs was diminished by two PI-PLC inhibitors and an IP3 receptor antagonist. These results evidence an ATP-sensitive SAC influx in MCF-7 cells and indicate that phosphorylation of MAPKs by ATP is dependent on PI-PLC/IP3/Ca2+i release but independent of SAC influx in these cells, differently to other cell types.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75907
Scodelaro Bilbao, Paola Gabriela; Boland, Ricardo Leopoldo; Russo, Ana Josefa; Santillán, Graciela Edith; ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 466; 1; 1-10-2007; 15-23
0003-9861
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75907
identifier_str_mv Scodelaro Bilbao, Paola Gabriela; Boland, Ricardo Leopoldo; Russo, Ana Josefa; Santillán, Graciela Edith; ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 466; 1; 1-10-2007; 15-23
0003-9861
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003986107003682
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.abb.2007.07.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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