DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10

Autores
Hu, Na; Strobl Mazulla, Pablo Hernan; Simoes Costa, Marcos; Sanchez Vasquez, Estefania; Bronner, Marianne E.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neural tube cells to generate emigrating neural crest cells. DNMT3B knockdown results in up-regulation of neural crest markers, prolonged neural crest emigration, and subsequent precocious neuronal differentiation of the trigeminal ganglion. We find that DNMT3B binds to the promoter of Sox10, known to be important for neural crest emigration and lineage acquisition. Bisulfite sequencing further reveals methylation of the Sox10 promoter region upon cessation of emigration in normal embryos, whereas this mark is reduced after DNMT3B loss. Taken together, these results reveal the importance of DNA methylation in regulating the ability of neural tube cells to produce neural crest cells and the timing of peripheral neuron differentiation.
Fil: Hu, Na. California Institute of Technology; Estados Unidos
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Simoes Costa, Marcos. California Institute of Technology; Estados Unidos
Fil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
Materia
Epigenetic
Neural Crest
Development
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34150
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34150
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10Hu, NaStrobl Mazulla, Pablo HernanSimoes Costa, MarcosSanchez Vasquez, EstefaniaBronner, Marianne E.EpigeneticNeural CrestDevelopmenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neural tube cells to generate emigrating neural crest cells. DNMT3B knockdown results in up-regulation of neural crest markers, prolonged neural crest emigration, and subsequent precocious neuronal differentiation of the trigeminal ganglion. We find that DNMT3B binds to the promoter of Sox10, known to be important for neural crest emigration and lineage acquisition. Bisulfite sequencing further reveals methylation of the Sox10 promoter region upon cessation of emigration in normal embryos, whereas this mark is reduced after DNMT3B loss. Taken together, these results reveal the importance of DNA methylation in regulating the ability of neural tube cells to produce neural crest cells and the timing of peripheral neuron differentiation.Fil: Hu, Na. California Institute of Technology; Estados UnidosFil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Simoes Costa, Marcos. California Institute of Technology; Estados UnidosFil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Bronner, Marianne E.. California Institute of Technology; Estados UnidosNational Academy of Sciences2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34150Hu, Na; Strobl Mazulla, Pablo Hernan; Simoes Costa, Marcos; Sanchez Vasquez, Estefania; Bronner, Marianne E.; DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 111; 50; 11-2014; 17911-179260027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1318408111info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/111/50/17911info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:37:45Zoai:ri.conicet.gov.ar:11336/34150instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:37:45.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
title DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
spellingShingle DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
Hu, Na
Epigenetic
Neural Crest
Development
title_short DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
title_full DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
title_fullStr DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
title_full_unstemmed DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
title_sort DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10
dc.creator.none.fl_str_mv Hu, Na
Strobl Mazulla, Pablo Hernan
Simoes Costa, Marcos
Sanchez Vasquez, Estefania
Bronner, Marianne E.
author Hu, Na
author_facet Hu, Na
Strobl Mazulla, Pablo Hernan
Simoes Costa, Marcos
Sanchez Vasquez, Estefania
Bronner, Marianne E.
author_role author
author2 Strobl Mazulla, Pablo Hernan
Simoes Costa, Marcos
Sanchez Vasquez, Estefania
Bronner, Marianne E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Epigenetic
Neural Crest
Development
topic Epigenetic
Neural Crest
Development
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neural tube cells to generate emigrating neural crest cells. DNMT3B knockdown results in up-regulation of neural crest markers, prolonged neural crest emigration, and subsequent precocious neuronal differentiation of the trigeminal ganglion. We find that DNMT3B binds to the promoter of Sox10, known to be important for neural crest emigration and lineage acquisition. Bisulfite sequencing further reveals methylation of the Sox10 promoter region upon cessation of emigration in normal embryos, whereas this mark is reduced after DNMT3B loss. Taken together, these results reveal the importance of DNA methylation in regulating the ability of neural tube cells to produce neural crest cells and the timing of peripheral neuron differentiation.
Fil: Hu, Na. California Institute of Technology; Estados Unidos
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Simoes Costa, Marcos. California Institute of Technology; Estados Unidos
Fil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
description Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neural tube cells to generate emigrating neural crest cells. DNMT3B knockdown results in up-regulation of neural crest markers, prolonged neural crest emigration, and subsequent precocious neuronal differentiation of the trigeminal ganglion. We find that DNMT3B binds to the promoter of Sox10, known to be important for neural crest emigration and lineage acquisition. Bisulfite sequencing further reveals methylation of the Sox10 promoter region upon cessation of emigration in normal embryos, whereas this mark is reduced after DNMT3B loss. Taken together, these results reveal the importance of DNA methylation in regulating the ability of neural tube cells to produce neural crest cells and the timing of peripheral neuron differentiation.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34150
Hu, Na; Strobl Mazulla, Pablo Hernan; Simoes Costa, Marcos; Sanchez Vasquez, Estefania; Bronner, Marianne E.; DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 111; 50; 11-2014; 17911-17926
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34150
identifier_str_mv Hu, Na; Strobl Mazulla, Pablo Hernan; Simoes Costa, Marcos; Sanchez Vasquez, Estefania; Bronner, Marianne E.; DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 111; 50; 11-2014; 17911-17926
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1318408111
info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/111/50/17911
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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