Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4

Autores
Peralta, Maria Fernanda; Magnoli, Alejandra Paola; Alustiza, Fabricio; Nilson, Armando Jesús; Miazzo, Raul Daniel; Vivas, Adriana Beatriz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Immunoglobulin Y (IgY) is the predominant antibody found in hen’s (Gallus domesticus) egg yolk. This antibody, developed against several microorganisms in hen egg yolk, has been successfully used as an alternative to immunoglobulins from mammals for use in immunodiagnostics and immunotherapy. Enteropathogenic Escherichia coli (E.coli) F4 is the main etiological agent associated with swine neonatal diarrhea, and it causes notable economic losses in swine production. The aim of the present study was to evaluate the relationship between humoral immune response and the activation of gut-associated lymphoid tissue (GALT) in laying hens intramuscularly immunized with E. coli F4. Adult laying Shaver hens were immunized with a bacterin based on an inactivated lysate E. coli F4 strain that was originally isolated from neonatal piglet diarrhea, following a recommended schedule. The percentage of B lymphocytes in blood and spleen homogenates was determined by flow cytometry. Villi histomorphometry and the size of germinal centers (GC) activated in GALT and the spleen were measured in histological samples either stained with hematoxylin/eosin or through immunofluorescence. Antibody and isotype-specific antibodies in serum and egg yolk were measured using indirect enzymelinked immunosorbent assay (ELISA). Secretory and serum immunoglobulin A (IgA) were measured by ELISA tests. Laying hen with intramuscular immunization with E. coli F4 lysate, activated both mucosal and systemic protection. Mucosal protection was provided through B lymphocytes, and most of them were activated on Peyer’s patches and esophageal tonsils, in GALT. Furthermore, increased B lymphocyte number in the lamina propria of the gut, and increased intraepithelial plasmatic cell number, produced high levels of mucosal IgA. Activated B lymphocytes interacted with absorptive cells, immune cells, and microbiota in the gut, producing signals that were translated into a powerful physical defense by producing a greater volume of mucin from an increased number of goblet cells. Systemic protection was provided through B lymphocyte activation of spleen GC, which produced hugely specific IgY serum levels. One week later, this specific IgY was deposited in the yolk. This suggests that GALT is a key immunologic tissue inside the mucosal immune system, acting as the “command center” for humoral reaction.
Fil: Peralta, Maria Fernanda. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Alustiza, Fabricio. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
Fil: Nilson, Armando Jesús. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina
Fil: Miazzo, Raul Daniel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina
Fil: Vivas, Adriana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
Materia
GUT-ASSOCIATED LYMPHATIC TISSUE
B LYMPHOCYTES
HEN
IMMUNOGLOBULIN Y TECHNOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157859

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network_name_str CONICET Digital (CONICET)
spelling Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4Peralta, Maria FernandaMagnoli, Alejandra PaolaAlustiza, FabricioNilson, Armando JesúsMiazzo, Raul DanielVivas, Adriana BeatrizGUT-ASSOCIATED LYMPHATIC TISSUEB LYMPHOCYTESHENIMMUNOGLOBULIN Y TECHNOLOGYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Immunoglobulin Y (IgY) is the predominant antibody found in hen’s (Gallus domesticus) egg yolk. This antibody, developed against several microorganisms in hen egg yolk, has been successfully used as an alternative to immunoglobulins from mammals for use in immunodiagnostics and immunotherapy. Enteropathogenic Escherichia coli (E.coli) F4 is the main etiological agent associated with swine neonatal diarrhea, and it causes notable economic losses in swine production. The aim of the present study was to evaluate the relationship between humoral immune response and the activation of gut-associated lymphoid tissue (GALT) in laying hens intramuscularly immunized with E. coli F4. Adult laying Shaver hens were immunized with a bacterin based on an inactivated lysate E. coli F4 strain that was originally isolated from neonatal piglet diarrhea, following a recommended schedule. The percentage of B lymphocytes in blood and spleen homogenates was determined by flow cytometry. Villi histomorphometry and the size of germinal centers (GC) activated in GALT and the spleen were measured in histological samples either stained with hematoxylin/eosin or through immunofluorescence. Antibody and isotype-specific antibodies in serum and egg yolk were measured using indirect enzymelinked immunosorbent assay (ELISA). Secretory and serum immunoglobulin A (IgA) were measured by ELISA tests. Laying hen with intramuscular immunization with E. coli F4 lysate, activated both mucosal and systemic protection. Mucosal protection was provided through B lymphocytes, and most of them were activated on Peyer’s patches and esophageal tonsils, in GALT. Furthermore, increased B lymphocyte number in the lamina propria of the gut, and increased intraepithelial plasmatic cell number, produced high levels of mucosal IgA. Activated B lymphocytes interacted with absorptive cells, immune cells, and microbiota in the gut, producing signals that were translated into a powerful physical defense by producing a greater volume of mucin from an increased number of goblet cells. Systemic protection was provided through B lymphocyte activation of spleen GC, which produced hugely specific IgY serum levels. One week later, this specific IgY was deposited in the yolk. This suggests that GALT is a key immunologic tissue inside the mucosal immune system, acting as the “command center” for humoral reaction.Fil: Peralta, Maria Fernanda. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; ArgentinaFil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; ArgentinaFil: Alustiza, Fabricio. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; ArgentinaFil: Nilson, Armando Jesús. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; ArgentinaFil: Miazzo, Raul Daniel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; ArgentinaFil: Vivas, Adriana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; ArgentinaFrontiers Media2017-05-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157859Peralta, Maria Fernanda; Magnoli, Alejandra Paola; Alustiza, Fabricio; Nilson, Armando Jesús; Miazzo, Raul Daniel; et al.; Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4; Frontiers Media; Frontiers in Immunology; 8; 568; 22-5-2017; 1-111664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.00568/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.00568info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:47Zoai:ri.conicet.gov.ar:11336/157859instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:47.433CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
title Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
spellingShingle Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
Peralta, Maria Fernanda
GUT-ASSOCIATED LYMPHATIC TISSUE
B LYMPHOCYTES
HEN
IMMUNOGLOBULIN Y TECHNOLOGY
title_short Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
title_full Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
title_fullStr Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
title_full_unstemmed Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
title_sort Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4
dc.creator.none.fl_str_mv Peralta, Maria Fernanda
Magnoli, Alejandra Paola
Alustiza, Fabricio
Nilson, Armando Jesús
Miazzo, Raul Daniel
Vivas, Adriana Beatriz
author Peralta, Maria Fernanda
author_facet Peralta, Maria Fernanda
Magnoli, Alejandra Paola
Alustiza, Fabricio
Nilson, Armando Jesús
Miazzo, Raul Daniel
Vivas, Adriana Beatriz
author_role author
author2 Magnoli, Alejandra Paola
Alustiza, Fabricio
Nilson, Armando Jesús
Miazzo, Raul Daniel
Vivas, Adriana Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GUT-ASSOCIATED LYMPHATIC TISSUE
B LYMPHOCYTES
HEN
IMMUNOGLOBULIN Y TECHNOLOGY
topic GUT-ASSOCIATED LYMPHATIC TISSUE
B LYMPHOCYTES
HEN
IMMUNOGLOBULIN Y TECHNOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Immunoglobulin Y (IgY) is the predominant antibody found in hen’s (Gallus domesticus) egg yolk. This antibody, developed against several microorganisms in hen egg yolk, has been successfully used as an alternative to immunoglobulins from mammals for use in immunodiagnostics and immunotherapy. Enteropathogenic Escherichia coli (E.coli) F4 is the main etiological agent associated with swine neonatal diarrhea, and it causes notable economic losses in swine production. The aim of the present study was to evaluate the relationship between humoral immune response and the activation of gut-associated lymphoid tissue (GALT) in laying hens intramuscularly immunized with E. coli F4. Adult laying Shaver hens were immunized with a bacterin based on an inactivated lysate E. coli F4 strain that was originally isolated from neonatal piglet diarrhea, following a recommended schedule. The percentage of B lymphocytes in blood and spleen homogenates was determined by flow cytometry. Villi histomorphometry and the size of germinal centers (GC) activated in GALT and the spleen were measured in histological samples either stained with hematoxylin/eosin or through immunofluorescence. Antibody and isotype-specific antibodies in serum and egg yolk were measured using indirect enzymelinked immunosorbent assay (ELISA). Secretory and serum immunoglobulin A (IgA) were measured by ELISA tests. Laying hen with intramuscular immunization with E. coli F4 lysate, activated both mucosal and systemic protection. Mucosal protection was provided through B lymphocytes, and most of them were activated on Peyer’s patches and esophageal tonsils, in GALT. Furthermore, increased B lymphocyte number in the lamina propria of the gut, and increased intraepithelial plasmatic cell number, produced high levels of mucosal IgA. Activated B lymphocytes interacted with absorptive cells, immune cells, and microbiota in the gut, producing signals that were translated into a powerful physical defense by producing a greater volume of mucin from an increased number of goblet cells. Systemic protection was provided through B lymphocyte activation of spleen GC, which produced hugely specific IgY serum levels. One week later, this specific IgY was deposited in the yolk. This suggests that GALT is a key immunologic tissue inside the mucosal immune system, acting as the “command center” for humoral reaction.
Fil: Peralta, Maria Fernanda. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Alustiza, Fabricio. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
Fil: Nilson, Armando Jesús. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina
Fil: Miazzo, Raul Daniel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina
Fil: Vivas, Adriana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
description Immunoglobulin Y (IgY) is the predominant antibody found in hen’s (Gallus domesticus) egg yolk. This antibody, developed against several microorganisms in hen egg yolk, has been successfully used as an alternative to immunoglobulins from mammals for use in immunodiagnostics and immunotherapy. Enteropathogenic Escherichia coli (E.coli) F4 is the main etiological agent associated with swine neonatal diarrhea, and it causes notable economic losses in swine production. The aim of the present study was to evaluate the relationship between humoral immune response and the activation of gut-associated lymphoid tissue (GALT) in laying hens intramuscularly immunized with E. coli F4. Adult laying Shaver hens were immunized with a bacterin based on an inactivated lysate E. coli F4 strain that was originally isolated from neonatal piglet diarrhea, following a recommended schedule. The percentage of B lymphocytes in blood and spleen homogenates was determined by flow cytometry. Villi histomorphometry and the size of germinal centers (GC) activated in GALT and the spleen were measured in histological samples either stained with hematoxylin/eosin or through immunofluorescence. Antibody and isotype-specific antibodies in serum and egg yolk were measured using indirect enzymelinked immunosorbent assay (ELISA). Secretory and serum immunoglobulin A (IgA) were measured by ELISA tests. Laying hen with intramuscular immunization with E. coli F4 lysate, activated both mucosal and systemic protection. Mucosal protection was provided through B lymphocytes, and most of them were activated on Peyer’s patches and esophageal tonsils, in GALT. Furthermore, increased B lymphocyte number in the lamina propria of the gut, and increased intraepithelial plasmatic cell number, produced high levels of mucosal IgA. Activated B lymphocytes interacted with absorptive cells, immune cells, and microbiota in the gut, producing signals that were translated into a powerful physical defense by producing a greater volume of mucin from an increased number of goblet cells. Systemic protection was provided through B lymphocyte activation of spleen GC, which produced hugely specific IgY serum levels. One week later, this specific IgY was deposited in the yolk. This suggests that GALT is a key immunologic tissue inside the mucosal immune system, acting as the “command center” for humoral reaction.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157859
Peralta, Maria Fernanda; Magnoli, Alejandra Paola; Alustiza, Fabricio; Nilson, Armando Jesús; Miazzo, Raul Daniel; et al.; Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4; Frontiers Media; Frontiers in Immunology; 8; 568; 22-5-2017; 1-11
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157859
identifier_str_mv Peralta, Maria Fernanda; Magnoli, Alejandra Paola; Alustiza, Fabricio; Nilson, Armando Jesús; Miazzo, Raul Daniel; et al.; Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4; Frontiers Media; Frontiers in Immunology; 8; 568; 22-5-2017; 1-11
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.00568/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.00568
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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