High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations

Autores
Forneris, Natalia Soledad; Otero, Gabriel; Pereyra, Ana; Repetto, Gustavo; Rabossi, Alejandro; Quesada Allue, Luis Alberto; Basso, Alicia L.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The horn fly, Haematobiairritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by Haematobiairritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of Haematobiairritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of Haematobiairritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination.
Fil: Forneris, Natalia Soledad. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Otero, Gabriel. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Pereyra, Ana. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Repetto, Gustavo. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Rabossi, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Quesada Allue, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Basso, Alicia L.. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Materia
KARYOTYPES
GENETIC VARIABILITY
POPULATION GENETICS
C-BANDING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9654
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9654
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populationsForneris, Natalia SoledadOtero, GabrielPereyra, AnaRepetto, GustavoRabossi, AlejandroQuesada Allue, Luis AlbertoBasso, Alicia L.KARYOTYPESGENETIC VARIABILITYPOPULATION GENETICSC-BANDINGhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The horn fly, Haematobiairritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by Haematobiairritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of Haematobiairritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of Haematobiairritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination.Fil: Forneris, Natalia Soledad. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Otero, Gabriel. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Pereyra, Ana. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Repetto, Gustavo. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Rabossi, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Quesada Allue, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Basso, Alicia L.. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaZoological Inst2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9654Forneris, Natalia Soledad; Otero, Gabriel; Pereyra, Ana; Repetto, Gustavo; Rabossi, Alejandro; et al.; High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations; Zoological Inst; Comparative Cytogenetics; 9; 1; 2-2015; 31-501993-0771enginfo:eu-repo/semantics/altIdentifier/url/http://compcytogen.pensoft.net/articles.php?id=4686info:eu-repo/semantics/altIdentifier/doi/10.3897/CompCytogen.v9i1.8535info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:33Zoai:ri.conicet.gov.ar:11336/9654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:33.462CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
title High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
spellingShingle High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
Forneris, Natalia Soledad
KARYOTYPES
GENETIC VARIABILITY
POPULATION GENETICS
C-BANDING
title_short High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
title_full High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
title_fullStr High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
title_full_unstemmed High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
title_sort High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations
dc.creator.none.fl_str_mv Forneris, Natalia Soledad
Otero, Gabriel
Pereyra, Ana
Repetto, Gustavo
Rabossi, Alejandro
Quesada Allue, Luis Alberto
Basso, Alicia L.
author Forneris, Natalia Soledad
author_facet Forneris, Natalia Soledad
Otero, Gabriel
Pereyra, Ana
Repetto, Gustavo
Rabossi, Alejandro
Quesada Allue, Luis Alberto
Basso, Alicia L.
author_role author
author2 Otero, Gabriel
Pereyra, Ana
Repetto, Gustavo
Rabossi, Alejandro
Quesada Allue, Luis Alberto
Basso, Alicia L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv KARYOTYPES
GENETIC VARIABILITY
POPULATION GENETICS
C-BANDING
topic KARYOTYPES
GENETIC VARIABILITY
POPULATION GENETICS
C-BANDING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The horn fly, Haematobiairritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by Haematobiairritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of Haematobiairritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of Haematobiairritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination.
Fil: Forneris, Natalia Soledad. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Otero, Gabriel. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Pereyra, Ana. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Repetto, Gustavo. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Rabossi, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Quesada Allue, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Basso, Alicia L.. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
description The horn fly, Haematobiairritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by Haematobiairritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of Haematobiairritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of Haematobiairritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9654
Forneris, Natalia Soledad; Otero, Gabriel; Pereyra, Ana; Repetto, Gustavo; Rabossi, Alejandro; et al.; High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations; Zoological Inst; Comparative Cytogenetics; 9; 1; 2-2015; 31-50
1993-0771
url http://hdl.handle.net/11336/9654
identifier_str_mv Forneris, Natalia Soledad; Otero, Gabriel; Pereyra, Ana; Repetto, Gustavo; Rabossi, Alejandro; et al.; High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations; Zoological Inst; Comparative Cytogenetics; 9; 1; 2-2015; 31-50
1993-0771
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://compcytogen.pensoft.net/articles.php?id=4686
info:eu-repo/semantics/altIdentifier/doi/10.3897/CompCytogen.v9i1.8535
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Zoological Inst
publisher.none.fl_str_mv Zoological Inst
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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