A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth

Autores
Battaglia, Marina Esther; Rípodas, Carolina; Clua, Joaquin; Baudin, Mael; Aguilar, Orlando Mario; Niebel, Andreas; Zanetti, María Eugenia; Blanco, Flavio Antonio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A C subunit of the heterotrimeric nuclear factor Y (NF-YC1) was shown to play a key role in nodule organogenesis and bacterialinfection during the nitrogenfixing symbiosis established between common bean (Phaseolus vulgaris) andRhizobium etli.Toidentify other proteins involved in this process, we used the yeast (Saccharomyces cerevisiae) two-hybrid system to screen for NF-YC1-interacting proteins. One of the positive clones encodes a member of the Phytochrome A Signal Transduction1 subfamily ofGRAS (for Gibberellic Acid-Insensitive (GAI), Repressor of GAI, and Scarecrow) transcription factors. The protein, namedScarecrow-like13 Involved in Nodulation (SIN1), localizes both to the nucleus and the cytoplasm, but in transgenicNicotianabenthamianacells, bimolecularfluorescence complementation suggested that the interaction with NF-YC1 takes placepredominantly in the nucleus. SIN1 is expressed in aerial and root tissues, with higher levels in roots and nodules.Posttranscriptional gene silencing ofSIN1using RNA interference (RNAi) showed that the product of this gene is involvedin lateral root elongation. However, root cell organization, density of lateral roots, and the length of root hairs were not affectedbySIN1RNAi. In addition, the expression of the RNAi ofSIN1led to a marked reduction in the number and size of nodulesformed upon inoculation withR. etliand affected the progression of infection threads toward the nodule primordia. ExpressionofNF-YA1and the G2/M transition cell cycle genesCYCLIN BandCell Division Cycle2was reduced inSIN1RNAi roots. Thesedata suggest that SIN1 plays a role in lateral root elongation and the establishment of root symbiosis in common bean.
Fil: Battaglia, Marina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Rípodas, Carolina. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Clua, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Baudin, Mael. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Niebel, Andreas. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Gras Transcription Factors
Nuclear Factor Y
Legumes
Nodulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32382
Acceder
id CONICETDig_050727f5aa48b56459e217e19ed346da
oai_identifier_str oai:ri.conicet.gov.ar:11336/32382
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growthBattaglia, Marina EstherRípodas, CarolinaClua, JoaquinBaudin, MaelAguilar, Orlando MarioNiebel, AndreasZanetti, María EugeniaBlanco, Flavio AntonioGras Transcription FactorsNuclear Factor YLegumesNodulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A C subunit of the heterotrimeric nuclear factor Y (NF-YC1) was shown to play a key role in nodule organogenesis and bacterialinfection during the nitrogenfixing symbiosis established between common bean (Phaseolus vulgaris) andRhizobium etli.Toidentify other proteins involved in this process, we used the yeast (Saccharomyces cerevisiae) two-hybrid system to screen for NF-YC1-interacting proteins. One of the positive clones encodes a member of the Phytochrome A Signal Transduction1 subfamily ofGRAS (for Gibberellic Acid-Insensitive (GAI), Repressor of GAI, and Scarecrow) transcription factors. The protein, namedScarecrow-like13 Involved in Nodulation (SIN1), localizes both to the nucleus and the cytoplasm, but in transgenicNicotianabenthamianacells, bimolecularfluorescence complementation suggested that the interaction with NF-YC1 takes placepredominantly in the nucleus. SIN1 is expressed in aerial and root tissues, with higher levels in roots and nodules.Posttranscriptional gene silencing ofSIN1using RNA interference (RNAi) showed that the product of this gene is involvedin lateral root elongation. However, root cell organization, density of lateral roots, and the length of root hairs were not affectedbySIN1RNAi. In addition, the expression of the RNAi ofSIN1led to a marked reduction in the number and size of nodulesformed upon inoculation withR. etliand affected the progression of infection threads toward the nodule primordia. ExpressionofNF-YA1and the G2/M transition cell cycle genesCYCLIN BandCell Division Cycle2was reduced inSIN1RNAi roots. Thesedata suggest that SIN1 plays a role in lateral root elongation and the establishment of root symbiosis in common bean.Fil: Battaglia, Marina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Rípodas, Carolina. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Clua, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Baudin, Mael. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Niebel, Andreas. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAmerican Society of Plant Biologist2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32382Battaglia, Marina Esther; Rípodas, Carolina; Clua, Joaquin; Baudin, Mael; Aguilar, Orlando Mario; et al.; A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth; American Society of Plant Biologist; Plant Physiology; 164; 3; 1-2014; 1430-14420032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.113.230896info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/3/1430info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:14Zoai:ri.conicet.gov.ar:11336/32382instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:14.32CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
title A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
spellingShingle A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
Battaglia, Marina Esther
Gras Transcription Factors
Nuclear Factor Y
Legumes
Nodulation
title_short A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
title_full A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
title_fullStr A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
title_full_unstemmed A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
title_sort A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth
dc.creator.none.fl_str_mv Battaglia, Marina Esther
Rípodas, Carolina
Clua, Joaquin
Baudin, Mael
Aguilar, Orlando Mario
Niebel, Andreas
Zanetti, María Eugenia
Blanco, Flavio Antonio
author Battaglia, Marina Esther
author_facet Battaglia, Marina Esther
Rípodas, Carolina
Clua, Joaquin
Baudin, Mael
Aguilar, Orlando Mario
Niebel, Andreas
Zanetti, María Eugenia
Blanco, Flavio Antonio
author_role author
author2 Rípodas, Carolina
Clua, Joaquin
Baudin, Mael
Aguilar, Orlando Mario
Niebel, Andreas
Zanetti, María Eugenia
Blanco, Flavio Antonio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gras Transcription Factors
Nuclear Factor Y
Legumes
Nodulation
topic Gras Transcription Factors
Nuclear Factor Y
Legumes
Nodulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A C subunit of the heterotrimeric nuclear factor Y (NF-YC1) was shown to play a key role in nodule organogenesis and bacterialinfection during the nitrogenfixing symbiosis established between common bean (Phaseolus vulgaris) andRhizobium etli.Toidentify other proteins involved in this process, we used the yeast (Saccharomyces cerevisiae) two-hybrid system to screen for NF-YC1-interacting proteins. One of the positive clones encodes a member of the Phytochrome A Signal Transduction1 subfamily ofGRAS (for Gibberellic Acid-Insensitive (GAI), Repressor of GAI, and Scarecrow) transcription factors. The protein, namedScarecrow-like13 Involved in Nodulation (SIN1), localizes both to the nucleus and the cytoplasm, but in transgenicNicotianabenthamianacells, bimolecularfluorescence complementation suggested that the interaction with NF-YC1 takes placepredominantly in the nucleus. SIN1 is expressed in aerial and root tissues, with higher levels in roots and nodules.Posttranscriptional gene silencing ofSIN1using RNA interference (RNAi) showed that the product of this gene is involvedin lateral root elongation. However, root cell organization, density of lateral roots, and the length of root hairs were not affectedbySIN1RNAi. In addition, the expression of the RNAi ofSIN1led to a marked reduction in the number and size of nodulesformed upon inoculation withR. etliand affected the progression of infection threads toward the nodule primordia. ExpressionofNF-YA1and the G2/M transition cell cycle genesCYCLIN BandCell Division Cycle2was reduced inSIN1RNAi roots. Thesedata suggest that SIN1 plays a role in lateral root elongation and the establishment of root symbiosis in common bean.
Fil: Battaglia, Marina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Rípodas, Carolina. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Clua, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Baudin, Mael. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Niebel, Andreas. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description A C subunit of the heterotrimeric nuclear factor Y (NF-YC1) was shown to play a key role in nodule organogenesis and bacterialinfection during the nitrogenfixing symbiosis established between common bean (Phaseolus vulgaris) andRhizobium etli.Toidentify other proteins involved in this process, we used the yeast (Saccharomyces cerevisiae) two-hybrid system to screen for NF-YC1-interacting proteins. One of the positive clones encodes a member of the Phytochrome A Signal Transduction1 subfamily ofGRAS (for Gibberellic Acid-Insensitive (GAI), Repressor of GAI, and Scarecrow) transcription factors. The protein, namedScarecrow-like13 Involved in Nodulation (SIN1), localizes both to the nucleus and the cytoplasm, but in transgenicNicotianabenthamianacells, bimolecularfluorescence complementation suggested that the interaction with NF-YC1 takes placepredominantly in the nucleus. SIN1 is expressed in aerial and root tissues, with higher levels in roots and nodules.Posttranscriptional gene silencing ofSIN1using RNA interference (RNAi) showed that the product of this gene is involvedin lateral root elongation. However, root cell organization, density of lateral roots, and the length of root hairs were not affectedbySIN1RNAi. In addition, the expression of the RNAi ofSIN1led to a marked reduction in the number and size of nodulesformed upon inoculation withR. etliand affected the progression of infection threads toward the nodule primordia. ExpressionofNF-YA1and the G2/M transition cell cycle genesCYCLIN BandCell Division Cycle2was reduced inSIN1RNAi roots. Thesedata suggest that SIN1 plays a role in lateral root elongation and the establishment of root symbiosis in common bean.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32382
Battaglia, Marina Esther; Rípodas, Carolina; Clua, Joaquin; Baudin, Mael; Aguilar, Orlando Mario; et al.; A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth; American Society of Plant Biologist; Plant Physiology; 164; 3; 1-2014; 1430-1442
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32382
identifier_str_mv Battaglia, Marina Esther; Rípodas, Carolina; Clua, Joaquin; Baudin, Mael; Aguilar, Orlando Mario; et al.; A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth; American Society of Plant Biologist; Plant Physiology; 164; 3; 1-2014; 1430-1442
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.113.230896
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/3/1430
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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