Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism

Autores
Moreno, Juan C.; Rojas, Bruno Ezequiel; Vicente, Rubén; Gorka, Michal; Matz, Timon; Chodasiewicz, Monika; Peralta?Ariza, Juan S.; Zhang, Youjun; Alseekh, Saleh; Childs, Dorothee; Luzarowski, Marcin; Nikoloski, Zoran; Zarivach, Raz; Walther, Dirk; Hartman, Matias Daniel; Figueroa, Carlos Maria; Iglesias, Alberto Alvaro; Fernie, Alisdair R.; Skirycz, Aleksandra
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Specifically, Tyr-Asp inhibits the activity of a key glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase (GAPC), and redirects glucose toward pentose phosphate pathway (PPP) and NADPH production. In line with the metabolic data, Tyr-Asp supplementation improved the growth performance of both Arabidopsis and tobacco seedlings subjected to oxidative stress conditions. Moreover, inhibition of Arabidopsis phosphoenolpyruvate carboxykinase (PEPCK) activity by a group of branched-chain amino acid-containing dipeptides, but not by Tyr-Asp, points to a multisite regulation of glycolytic/gluconeogenic pathway by dipeptides. In summary, our results open the intriguing possibility that proteogenic dipeptides act as evolutionarily conserved small-molecule regulators at the nexus of stress, protein degradation, and metabolism.
Fil: Moreno, Juan C.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Rojas, Bruno Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Vicente, Rubén. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Gorka, Michal. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Matz, Timon. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Chodasiewicz, Monika. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Peralta?Ariza, Juan S.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Zhang, Youjun. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Alseekh, Saleh. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Childs, Dorothee. European Molecular Biology Laboratory; Alemania
Fil: Luzarowski, Marcin. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Nikoloski, Zoran. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Zarivach, Raz. Ben Gurion University of the Negev; Israel
Fil: Walther, Dirk. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Hartman, Matias Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Fernie, Alisdair R.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Skirycz, Aleksandra. Max Planck Institute Of Molecular Plant Physiology; Alemania
Materia
ARABIDOPSIS
CENTRAL CARBON METABOLISM
DIPEPTIDES
GAPDH
NADPH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184996
Acceder
id CONICETDig_03fe7bb7cf459923195fabc4d5f53627
oai_identifier_str oai:ri.conicet.gov.ar:11336/184996
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolismMoreno, Juan C.Rojas, Bruno EzequielVicente, RubénGorka, MichalMatz, TimonChodasiewicz, MonikaPeralta?Ariza, Juan S.Zhang, YoujunAlseekh, SalehChilds, DorotheeLuzarowski, MarcinNikoloski, ZoranZarivach, RazWalther, DirkHartman, Matias DanielFigueroa, Carlos MariaIglesias, Alberto AlvaroFernie, Alisdair R.Skirycz, AleksandraARABIDOPSISCENTRAL CARBON METABOLISMDIPEPTIDESGAPDHNADPHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Specifically, Tyr-Asp inhibits the activity of a key glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase (GAPC), and redirects glucose toward pentose phosphate pathway (PPP) and NADPH production. In line with the metabolic data, Tyr-Asp supplementation improved the growth performance of both Arabidopsis and tobacco seedlings subjected to oxidative stress conditions. Moreover, inhibition of Arabidopsis phosphoenolpyruvate carboxykinase (PEPCK) activity by a group of branched-chain amino acid-containing dipeptides, but not by Tyr-Asp, points to a multisite regulation of glycolytic/gluconeogenic pathway by dipeptides. In summary, our results open the intriguing possibility that proteogenic dipeptides act as evolutionarily conserved small-molecule regulators at the nexus of stress, protein degradation, and metabolism.Fil: Moreno, Juan C.. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Rojas, Bruno Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Vicente, Rubén. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Gorka, Michal. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Matz, Timon. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Chodasiewicz, Monika. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Peralta?Ariza, Juan S.. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Zhang, Youjun. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Alseekh, Saleh. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Childs, Dorothee. European Molecular Biology Laboratory; AlemaniaFil: Luzarowski, Marcin. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Nikoloski, Zoran. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Zarivach, Raz. Ben Gurion University of the Negev; IsraelFil: Walther, Dirk. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Hartman, Matias Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Fernie, Alisdair R.. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Skirycz, Aleksandra. Max Planck Institute Of Molecular Plant Physiology; AlemaniaNature Publishing Group2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184996Moreno, Juan C.; Rojas, Bruno Ezequiel; Vicente, Rubén; Gorka, Michal; Matz, Timon; et al.; Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism; Nature Publishing Group; Embo Journal; 40; 15; 6-2021; 1-160261-4189CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.15252/embj.2020106800info:eu-repo/semantics/altIdentifier/doi/10.15252/embj.2020106800info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:21Zoai:ri.conicet.gov.ar:11336/184996instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:22.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
title Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
spellingShingle Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
Moreno, Juan C.
ARABIDOPSIS
CENTRAL CARBON METABOLISM
DIPEPTIDES
GAPDH
NADPH
title_short Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
title_full Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
title_fullStr Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
title_full_unstemmed Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
title_sort Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism
dc.creator.none.fl_str_mv Moreno, Juan C.
Rojas, Bruno Ezequiel
Vicente, Rubén
Gorka, Michal
Matz, Timon
Chodasiewicz, Monika
Peralta?Ariza, Juan S.
Zhang, Youjun
Alseekh, Saleh
Childs, Dorothee
Luzarowski, Marcin
Nikoloski, Zoran
Zarivach, Raz
Walther, Dirk
Hartman, Matias Daniel
Figueroa, Carlos Maria
Iglesias, Alberto Alvaro
Fernie, Alisdair R.
Skirycz, Aleksandra
author Moreno, Juan C.
author_facet Moreno, Juan C.
Rojas, Bruno Ezequiel
Vicente, Rubén
Gorka, Michal
Matz, Timon
Chodasiewicz, Monika
Peralta?Ariza, Juan S.
Zhang, Youjun
Alseekh, Saleh
Childs, Dorothee
Luzarowski, Marcin
Nikoloski, Zoran
Zarivach, Raz
Walther, Dirk
Hartman, Matias Daniel
Figueroa, Carlos Maria
Iglesias, Alberto Alvaro
Fernie, Alisdair R.
Skirycz, Aleksandra
author_role author
author2 Rojas, Bruno Ezequiel
Vicente, Rubén
Gorka, Michal
Matz, Timon
Chodasiewicz, Monika
Peralta?Ariza, Juan S.
Zhang, Youjun
Alseekh, Saleh
Childs, Dorothee
Luzarowski, Marcin
Nikoloski, Zoran
Zarivach, Raz
Walther, Dirk
Hartman, Matias Daniel
Figueroa, Carlos Maria
Iglesias, Alberto Alvaro
Fernie, Alisdair R.
Skirycz, Aleksandra
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
CENTRAL CARBON METABOLISM
DIPEPTIDES
GAPDH
NADPH
topic ARABIDOPSIS
CENTRAL CARBON METABOLISM
DIPEPTIDES
GAPDH
NADPH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Specifically, Tyr-Asp inhibits the activity of a key glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase (GAPC), and redirects glucose toward pentose phosphate pathway (PPP) and NADPH production. In line with the metabolic data, Tyr-Asp supplementation improved the growth performance of both Arabidopsis and tobacco seedlings subjected to oxidative stress conditions. Moreover, inhibition of Arabidopsis phosphoenolpyruvate carboxykinase (PEPCK) activity by a group of branched-chain amino acid-containing dipeptides, but not by Tyr-Asp, points to a multisite regulation of glycolytic/gluconeogenic pathway by dipeptides. In summary, our results open the intriguing possibility that proteogenic dipeptides act as evolutionarily conserved small-molecule regulators at the nexus of stress, protein degradation, and metabolism.
Fil: Moreno, Juan C.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Rojas, Bruno Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Vicente, Rubén. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Gorka, Michal. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Matz, Timon. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Chodasiewicz, Monika. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Peralta?Ariza, Juan S.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Zhang, Youjun. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Alseekh, Saleh. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Childs, Dorothee. European Molecular Biology Laboratory; Alemania
Fil: Luzarowski, Marcin. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Nikoloski, Zoran. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Zarivach, Raz. Ben Gurion University of the Negev; Israel
Fil: Walther, Dirk. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Hartman, Matias Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Fernie, Alisdair R.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Skirycz, Aleksandra. Max Planck Institute Of Molecular Plant Physiology; Alemania
description How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Specifically, Tyr-Asp inhibits the activity of a key glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase (GAPC), and redirects glucose toward pentose phosphate pathway (PPP) and NADPH production. In line with the metabolic data, Tyr-Asp supplementation improved the growth performance of both Arabidopsis and tobacco seedlings subjected to oxidative stress conditions. Moreover, inhibition of Arabidopsis phosphoenolpyruvate carboxykinase (PEPCK) activity by a group of branched-chain amino acid-containing dipeptides, but not by Tyr-Asp, points to a multisite regulation of glycolytic/gluconeogenic pathway by dipeptides. In summary, our results open the intriguing possibility that proteogenic dipeptides act as evolutionarily conserved small-molecule regulators at the nexus of stress, protein degradation, and metabolism.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184996
Moreno, Juan C.; Rojas, Bruno Ezequiel; Vicente, Rubén; Gorka, Michal; Matz, Timon; et al.; Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism; Nature Publishing Group; Embo Journal; 40; 15; 6-2021; 1-16
0261-4189
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184996
identifier_str_mv Moreno, Juan C.; Rojas, Bruno Ezequiel; Vicente, Rubén; Gorka, Michal; Matz, Timon; et al.; Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism; Nature Publishing Group; Embo Journal; 40; 15; 6-2021; 1-16
0261-4189
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.15252/embj.2020106800
info:eu-repo/semantics/altIdentifier/doi/10.15252/embj.2020106800
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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