Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
- Autores
- Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; Leguizamón, S
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.
Fil: Sartor, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina
Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leguizamón, S. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina - Materia
-
Chagas Disease
Trans-Sialidase
Sylvatic Reservoirs
Diagnosis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/19807
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Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal SpeciesSartor, Paula AndreaCeballos, Leonardo A.Orozco, Maria MarcelaCardinal, Marta VictoriaGurtler, Ricardo EstebanLeguizamón, SChagas DiseaseTrans-SialidaseSylvatic ReservoirsDiagnosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.Fil: Sartor, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; ArgentinaFil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leguizamón, S. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaMary Ann Liebert Inc2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19807Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; et al.; Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species; Mary Ann Liebert Inc; Vector-borne And Zoonotic Diseases; 13; 8; 5-2013; 581-5851530-3667CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/vbz.2012.1125info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/vbz.2012.1125info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:18Zoai:ri.conicet.gov.ar:11336/19807instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:18.727CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
title |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
spellingShingle |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species Sartor, Paula Andrea Chagas Disease Trans-Sialidase Sylvatic Reservoirs Diagnosis |
title_short |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
title_full |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
title_fullStr |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
title_full_unstemmed |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
title_sort |
Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species |
dc.creator.none.fl_str_mv |
Sartor, Paula Andrea Ceballos, Leonardo A. Orozco, Maria Marcela Cardinal, Marta Victoria Gurtler, Ricardo Esteban Leguizamón, S |
author |
Sartor, Paula Andrea |
author_facet |
Sartor, Paula Andrea Ceballos, Leonardo A. Orozco, Maria Marcela Cardinal, Marta Victoria Gurtler, Ricardo Esteban Leguizamón, S |
author_role |
author |
author2 |
Ceballos, Leonardo A. Orozco, Maria Marcela Cardinal, Marta Victoria Gurtler, Ricardo Esteban Leguizamón, S |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Chagas Disease Trans-Sialidase Sylvatic Reservoirs Diagnosis |
topic |
Chagas Disease Trans-Sialidase Sylvatic Reservoirs Diagnosis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts. Fil: Sartor, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leguizamón, S. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina |
description |
The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/19807 Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; et al.; Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species; Mary Ann Liebert Inc; Vector-borne And Zoonotic Diseases; 13; 8; 5-2013; 581-585 1530-3667 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/19807 |
identifier_str_mv |
Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; et al.; Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species; Mary Ann Liebert Inc; Vector-borne And Zoonotic Diseases; 13; 8; 5-2013; 581-585 1530-3667 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1089/vbz.2012.1125 info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/vbz.2012.1125 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert Inc |
publisher.none.fl_str_mv |
Mary Ann Liebert Inc |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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