Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species

Autores
Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; Leguizamón, S
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.
Fil: Sartor, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina
Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leguizamón, S. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Materia
Chagas Disease
Trans-Sialidase
Sylvatic Reservoirs
Diagnosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19807

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network_name_str CONICET Digital (CONICET)
spelling Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal SpeciesSartor, Paula AndreaCeballos, Leonardo A.Orozco, Maria MarcelaCardinal, Marta VictoriaGurtler, Ricardo EstebanLeguizamón, SChagas DiseaseTrans-SialidaseSylvatic ReservoirsDiagnosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.Fil: Sartor, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; ArgentinaFil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leguizamón, S. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaMary Ann Liebert Inc2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19807Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; et al.; Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species; Mary Ann Liebert Inc; Vector-borne And Zoonotic Diseases; 13; 8; 5-2013; 581-5851530-3667CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/vbz.2012.1125info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/vbz.2012.1125info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:18Zoai:ri.conicet.gov.ar:11336/19807instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:18.727CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
title Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
spellingShingle Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
Sartor, Paula Andrea
Chagas Disease
Trans-Sialidase
Sylvatic Reservoirs
Diagnosis
title_short Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
title_full Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
title_fullStr Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
title_full_unstemmed Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
title_sort Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
dc.creator.none.fl_str_mv Sartor, Paula Andrea
Ceballos, Leonardo A.
Orozco, Maria Marcela
Cardinal, Marta Victoria
Gurtler, Ricardo Esteban
Leguizamón, S
author Sartor, Paula Andrea
author_facet Sartor, Paula Andrea
Ceballos, Leonardo A.
Orozco, Maria Marcela
Cardinal, Marta Victoria
Gurtler, Ricardo Esteban
Leguizamón, S
author_role author
author2 Ceballos, Leonardo A.
Orozco, Maria Marcela
Cardinal, Marta Victoria
Gurtler, Ricardo Esteban
Leguizamón, S
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Trans-Sialidase
Sylvatic Reservoirs
Diagnosis
topic Chagas Disease
Trans-Sialidase
Sylvatic Reservoirs
Diagnosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.
Fil: Sartor, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina
Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leguizamón, S. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
description The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and conegativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19807
Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; et al.; Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species; Mary Ann Liebert Inc; Vector-borne And Zoonotic Diseases; 13; 8; 5-2013; 581-585
1530-3667
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19807
identifier_str_mv Sartor, Paula Andrea; Ceballos, Leonardo A.; Orozco, Maria Marcela; Cardinal, Marta Victoria; Gurtler, Ricardo Esteban; et al.; Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species; Mary Ann Liebert Inc; Vector-borne And Zoonotic Diseases; 13; 8; 5-2013; 581-585
1530-3667
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Mary Ann Liebert Inc
publisher.none.fl_str_mv Mary Ann Liebert Inc
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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