Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina...

Autores
Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; Nonaka, Shigeo; Basombrío, Miguel Ángel Manuel; Taranto, Nestor Juan; Hashiguchi, Yoshihisa
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón
Fil: Uezato, Hiroshi. Universidad de Ryukyus. Okinawa; Japón
Fil: Mimori, Tatsuyuki. Universidad de Kumamoto; Japón
Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Kochi University. Kochi Medical School; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón
Fil: Nonaka, Shigeo. Universidad de Ryukyus, Okinawa.; Japón
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Taranto, Nestor Juan. Instituto de Enfermedades Tropicales. Sede Regional Oran. Universidad Nacional de Salta; Argentina
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón
Materia
Citocromo B
Ps-Pcr
Argentina
Lesihmania
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43278
Acceder
id CONICETDig_023b4ecdff318009cdabf9b64ae7210b
oai_identifier_str oai:ri.conicet.gov.ar:11336/43278
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?Marco, Jorge DiegoUezato, HiroshiMimori, TatsuyukiBarroso, Paola AndreaKorenaga, MasatakaNonaka, ShigeoBasombrío, Miguel Ángel ManuelTaranto, Nestor JuanHashiguchi, YoshihisaCitocromo BPs-PcrArgentinaLesihmaniahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; JapónFil: Uezato, Hiroshi. Universidad de Ryukyus. Okinawa; JapónFil: Mimori, Tatsuyuki. Universidad de Kumamoto; JapónFil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Kochi University. Kochi Medical School; JapónFil: Korenaga, Masataka. Kochi University. Kochi Medical School; JapónFil: Nonaka, Shigeo. Universidad de Ryukyus, Okinawa.; JapónFil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Taranto, Nestor Juan. Instituto de Enfermedades Tropicales. Sede Regional Oran. Universidad Nacional de Salta; ArgentinaFil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; JapónAmerican Society of Tropical Medicine and Hygiene2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43278Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-2600002-96371476-1645CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2006.75.256#html_fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:34:35Zoai:ri.conicet.gov.ar:11336/43278instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:34:35.69CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
title Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
spellingShingle Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
Marco, Jorge Diego
Citocromo B
Ps-Pcr
Argentina
Lesihmania
title_short Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
title_full Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
title_fullStr Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
title_full_unstemmed Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
title_sort Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
dc.creator.none.fl_str_mv Marco, Jorge Diego
Uezato, Hiroshi
Mimori, Tatsuyuki
Barroso, Paola Andrea
Korenaga, Masataka
Nonaka, Shigeo
Basombrío, Miguel Ángel Manuel
Taranto, Nestor Juan
Hashiguchi, Yoshihisa
author Marco, Jorge Diego
author_facet Marco, Jorge Diego
Uezato, Hiroshi
Mimori, Tatsuyuki
Barroso, Paola Andrea
Korenaga, Masataka
Nonaka, Shigeo
Basombrío, Miguel Ángel Manuel
Taranto, Nestor Juan
Hashiguchi, Yoshihisa
author_role author
author2 Uezato, Hiroshi
Mimori, Tatsuyuki
Barroso, Paola Andrea
Korenaga, Masataka
Nonaka, Shigeo
Basombrío, Miguel Ángel Manuel
Taranto, Nestor Juan
Hashiguchi, Yoshihisa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citocromo B
Ps-Pcr
Argentina
Lesihmania
topic Citocromo B
Ps-Pcr
Argentina
Lesihmania
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón
Fil: Uezato, Hiroshi. Universidad de Ryukyus. Okinawa; Japón
Fil: Mimori, Tatsuyuki. Universidad de Kumamoto; Japón
Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Kochi University. Kochi Medical School; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón
Fil: Nonaka, Shigeo. Universidad de Ryukyus, Okinawa.; Japón
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Taranto, Nestor Juan. Instituto de Enfermedades Tropicales. Sede Regional Oran. Universidad Nacional de Salta; Argentina
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón
description Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43278
Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-260
0002-9637
1476-1645
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43278
identifier_str_mv Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-260
0002-9637
1476-1645
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2006.75.256#html_fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781972790116352
score 12.982451

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