Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina...
- Autores
- Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; Nonaka, Shigeo; Basombrío, Miguel Ángel Manuel; Taranto, Nestor Juan; Hashiguchi, Yoshihisa
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón
Fil: Uezato, Hiroshi. Universidad de Ryukyus. Okinawa; Japón
Fil: Mimori, Tatsuyuki. Universidad de Kumamoto; Japón
Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Kochi University. Kochi Medical School; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón
Fil: Nonaka, Shigeo. Universidad de Ryukyus, Okinawa.; Japón
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Taranto, Nestor Juan. Instituto de Enfermedades Tropicales. Sede Regional Oran. Universidad Nacional de Salta; Argentina
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón - Materia
-
Citocromo B
Ps-Pcr
Argentina
Lesihmania - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/43278
Ver los metadatos del registro completo
id |
CONICETDig_023b4ecdff318009cdabf9b64ae7210b |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/43278 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?Marco, Jorge DiegoUezato, HiroshiMimori, TatsuyukiBarroso, Paola AndreaKorenaga, MasatakaNonaka, ShigeoBasombrío, Miguel Ángel ManuelTaranto, Nestor JuanHashiguchi, YoshihisaCitocromo BPs-PcrArgentinaLesihmaniahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; JapónFil: Uezato, Hiroshi. Universidad de Ryukyus. Okinawa; JapónFil: Mimori, Tatsuyuki. Universidad de Kumamoto; JapónFil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Kochi University. Kochi Medical School; JapónFil: Korenaga, Masataka. Kochi University. Kochi Medical School; JapónFil: Nonaka, Shigeo. Universidad de Ryukyus, Okinawa.; JapónFil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Taranto, Nestor Juan. Instituto de Enfermedades Tropicales. Sede Regional Oran. Universidad Nacional de Salta; ArgentinaFil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; JapónAmerican Society of Tropical Medicine and Hygiene2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43278Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-2600002-96371476-1645CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2006.75.256#html_fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:54Zoai:ri.conicet.gov.ar:11336/43278instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:54.807CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
title |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
spellingShingle |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? Marco, Jorge Diego Citocromo B Ps-Pcr Argentina Lesihmania |
title_short |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
title_full |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
title_fullStr |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
title_full_unstemmed |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
title_sort |
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? |
dc.creator.none.fl_str_mv |
Marco, Jorge Diego Uezato, Hiroshi Mimori, Tatsuyuki Barroso, Paola Andrea Korenaga, Masataka Nonaka, Shigeo Basombrío, Miguel Ángel Manuel Taranto, Nestor Juan Hashiguchi, Yoshihisa |
author |
Marco, Jorge Diego |
author_facet |
Marco, Jorge Diego Uezato, Hiroshi Mimori, Tatsuyuki Barroso, Paola Andrea Korenaga, Masataka Nonaka, Shigeo Basombrío, Miguel Ángel Manuel Taranto, Nestor Juan Hashiguchi, Yoshihisa |
author_role |
author |
author2 |
Uezato, Hiroshi Mimori, Tatsuyuki Barroso, Paola Andrea Korenaga, Masataka Nonaka, Shigeo Basombrío, Miguel Ángel Manuel Taranto, Nestor Juan Hashiguchi, Yoshihisa |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Citocromo B Ps-Pcr Argentina Lesihmania |
topic |
Citocromo B Ps-Pcr Argentina Lesihmania |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country. Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón Fil: Uezato, Hiroshi. Universidad de Ryukyus. Okinawa; Japón Fil: Mimori, Tatsuyuki. Universidad de Kumamoto; Japón Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Kochi University. Kochi Medical School; Japón Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón Fil: Nonaka, Shigeo. Universidad de Ryukyus, Okinawa.; Japón Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Taranto, Nestor Juan. Instituto de Enfermedades Tropicales. Sede Regional Oran. Universidad Nacional de Salta; Argentina Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón |
description |
Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/43278 Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-260 0002-9637 1476-1645 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/43278 |
identifier_str_mv |
Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-260 0002-9637 1476-1645 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2006.75.256#html_fulltext |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Tropical Medicine and Hygiene |
publisher.none.fl_str_mv |
American Society of Tropical Medicine and Hygiene |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613879105060864 |
score |
13.070432 |