Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources

Autores
Durrieu, Lucía; Bush, Alan; Grande, Alicia Viviana; Rikard, Johansson; David, Janzén; Katz, Andrea; Cedersund, Gunnar; Colman Lerner, Alejandro Ariel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nuclear transport is an essential part of eukaryotic cell function. Here, we present scFRAP, a model-assisted fluorescent recovery after photobleaching (FRAP)- based method to determine nuclear import and export rates independently in individual live cells. To overcome the inherent noise of single-cell measurements, we performed sequential FRAPs on the same cell. We found large cell-to-cell variation in transport rates within isogenic yeast populations. For passive transport, the variability in NPC number might explain most of the variability. Using this approach, we studied mother-daughter cell asymmetry in the active nuclear shuttling of the transcription factor Ace2, which is specifically concentrated in daughter cell nuclei in early G1. Rather than reduced export in the daughter cell, as previously hypothesized, we found that this asymmetry is mainly due to an increased import in daughters. These results shed light on cell-to-cell variation in cellular dynamics and its sources.
Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Grande, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Rikard, Johansson. Linköping University; Suecia
Fil: David, Janzén. Linköping University; Suecia
Fil: Katz, Andrea. Universidad de Buenos Aires; Argentina
Fil: Cedersund, Gunnar. Linköping University; Suecia
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
BIOLOGICAL SCIENCES
CELL BIOLOGY
MATHEMATICAL BIOSCIENCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228376
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228376
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of cell-to-cell variation in nuclear transport rates and identification of its sourcesDurrieu, LucíaBush, AlanGrande, Alicia VivianaRikard, JohanssonDavid, JanzénKatz, AndreaCedersund, GunnarColman Lerner, Alejandro ArielBIOLOGICAL SCIENCESCELL BIOLOGYMATHEMATICAL BIOSCIENCEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nuclear transport is an essential part of eukaryotic cell function. Here, we present scFRAP, a model-assisted fluorescent recovery after photobleaching (FRAP)- based method to determine nuclear import and export rates independently in individual live cells. To overcome the inherent noise of single-cell measurements, we performed sequential FRAPs on the same cell. We found large cell-to-cell variation in transport rates within isogenic yeast populations. For passive transport, the variability in NPC number might explain most of the variability. Using this approach, we studied mother-daughter cell asymmetry in the active nuclear shuttling of the transcription factor Ace2, which is specifically concentrated in daughter cell nuclei in early G1. Rather than reduced export in the daughter cell, as previously hypothesized, we found that this asymmetry is mainly due to an increased import in daughters. These results shed light on cell-to-cell variation in cellular dynamics and its sources.Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Grande, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Rikard, Johansson. Linköping University; SueciaFil: David, Janzén. Linköping University; SueciaFil: Katz, Andrea. Universidad de Buenos Aires; ArgentinaFil: Cedersund, Gunnar. Linköping University; SueciaFil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaElsevier2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228376Durrieu, Lucía; Bush, Alan; Grande, Alicia Viviana; Rikard, Johansson; David, Janzén; et al.; Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources; Elsevier; iScience; 26; 1; 1-2023; 1-202589-0042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2022.105906info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:53Zoai:ri.conicet.gov.ar:11336/228376instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:54.001CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
title Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
spellingShingle Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
Durrieu, Lucía
BIOLOGICAL SCIENCES
CELL BIOLOGY
MATHEMATICAL BIOSCIENCES
title_short Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
title_full Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
title_fullStr Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
title_full_unstemmed Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
title_sort Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
dc.creator.none.fl_str_mv Durrieu, Lucía
Bush, Alan
Grande, Alicia Viviana
Rikard, Johansson
David, Janzén
Katz, Andrea
Cedersund, Gunnar
Colman Lerner, Alejandro Ariel
author Durrieu, Lucía
author_facet Durrieu, Lucía
Bush, Alan
Grande, Alicia Viviana
Rikard, Johansson
David, Janzén
Katz, Andrea
Cedersund, Gunnar
Colman Lerner, Alejandro Ariel
author_role author
author2 Bush, Alan
Grande, Alicia Viviana
Rikard, Johansson
David, Janzén
Katz, Andrea
Cedersund, Gunnar
Colman Lerner, Alejandro Ariel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL SCIENCES
CELL BIOLOGY
MATHEMATICAL BIOSCIENCES
topic BIOLOGICAL SCIENCES
CELL BIOLOGY
MATHEMATICAL BIOSCIENCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nuclear transport is an essential part of eukaryotic cell function. Here, we present scFRAP, a model-assisted fluorescent recovery after photobleaching (FRAP)- based method to determine nuclear import and export rates independently in individual live cells. To overcome the inherent noise of single-cell measurements, we performed sequential FRAPs on the same cell. We found large cell-to-cell variation in transport rates within isogenic yeast populations. For passive transport, the variability in NPC number might explain most of the variability. Using this approach, we studied mother-daughter cell asymmetry in the active nuclear shuttling of the transcription factor Ace2, which is specifically concentrated in daughter cell nuclei in early G1. Rather than reduced export in the daughter cell, as previously hypothesized, we found that this asymmetry is mainly due to an increased import in daughters. These results shed light on cell-to-cell variation in cellular dynamics and its sources.
Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Grande, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Rikard, Johansson. Linköping University; Suecia
Fil: David, Janzén. Linköping University; Suecia
Fil: Katz, Andrea. Universidad de Buenos Aires; Argentina
Fil: Cedersund, Gunnar. Linköping University; Suecia
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Nuclear transport is an essential part of eukaryotic cell function. Here, we present scFRAP, a model-assisted fluorescent recovery after photobleaching (FRAP)- based method to determine nuclear import and export rates independently in individual live cells. To overcome the inherent noise of single-cell measurements, we performed sequential FRAPs on the same cell. We found large cell-to-cell variation in transport rates within isogenic yeast populations. For passive transport, the variability in NPC number might explain most of the variability. Using this approach, we studied mother-daughter cell asymmetry in the active nuclear shuttling of the transcription factor Ace2, which is specifically concentrated in daughter cell nuclei in early G1. Rather than reduced export in the daughter cell, as previously hypothesized, we found that this asymmetry is mainly due to an increased import in daughters. These results shed light on cell-to-cell variation in cellular dynamics and its sources.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228376
Durrieu, Lucía; Bush, Alan; Grande, Alicia Viviana; Rikard, Johansson; David, Janzén; et al.; Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources; Elsevier; iScience; 26; 1; 1-2023; 1-20
2589-0042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228376
identifier_str_mv Durrieu, Lucía; Bush, Alan; Grande, Alicia Viviana; Rikard, Johansson; David, Janzén; et al.; Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources; Elsevier; iScience; 26; 1; 1-2023; 1-20
2589-0042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2022.105906
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082983155466240
score 13.22299

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