Genetic mapping of high caries experience on human chromosome 13
- Autores
- Küchler, Erika C.; Deeley, Kathleen; Ho, Bao; Linkowski, Samantha; Meyer, Chelsea; Noel, Jacqueline; Kouzbari, M. Zahir; Bezamat, Mariana; Granjeiro, José M.; Antunes, Leonardo S.; Antunes, Livia Azeredo; Abreu, Fernanda Volpe de; Costa, Marcelo C.; Tannure, Patricia N.; Seymen, Figen; Koruyucu, Mine; Patir, Asli; Mereb, Juan C.; Poletta, Fernando Adrián; Castilla, Eduardo Enrique; Orioli, Ieda M.; Marazita, Mary L.; Vieira, Alexandre R.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Our previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries. Methods: Seventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals. Results: Statistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence. Conclusions: Genetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored.
Fil: Küchler, Erika C.. University of Pittsburgh; Estados Unidos
Fil: Deeley, Kathleen. University of Pittsburgh; Estados Unidos
Fil: Ho, Bao. University of Pittsburgh; Estados Unidos
Fil: Linkowski, Samantha. University of Pittsburgh; Estados Unidos
Fil: Meyer, Chelsea. University of Pittsburgh; Estados Unidos
Fil: Noel, Jacqueline. University of Pittsburgh; Estados Unidos
Fil: Kouzbari, M. Zahir. University of Pittsburgh; Estados Unidos
Fil: Bezamat, Mariana. University of Pittsburgh; Estados Unidos
Fil: Granjeiro, José M.. Universidade Federal Fluminense; Brasil
Fil: Antunes, Leonardo S.. Universidade Federal Fluminense; Brasil
Fil: Antunes, Livia Azeredo. Universidade Federal Fluminense; Brasil
Fil: Abreu, Fernanda Volpe de. Universidade Federal Fluminense; Brasil
Fil: Costa, Marcelo C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Tannure, Patricia N.. Veiga de Almeida; Brasil. Salgado de Oliveira University; Brasil
Fil: Seymen, Figen. Istanbul University; Turquía
Fil: Koruyucu, Mine. Istanbul University; Turquía
Fil: Patir, Asli. Istanbul University; Turquía
Fil: Mereb, Juan C.. Estudio Colaborativo Latinoamericano de Malformaciones Congénitas; Argentina. Hospital de Área El Boóon; Argentina
Fil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina. Oswaldo Cruz Foundation; Brasil
Fil: Castilla, Eduardo Enrique. Oswaldo Cruz Foundation; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina
Fil: Orioli, Ieda M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Marazita, Mary L.. University of Pittsburgh; Estados Unidos
Fil: Vieira, Alexandre R.. University of Pittsburgh; Estados Unidos - Materia
-
Genetics
Oral health
Polymorphism
Caries - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/23882
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/23882 |
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Genetic mapping of high caries experience on human chromosome 13Küchler, Erika C.Deeley, KathleenHo, BaoLinkowski, SamanthaMeyer, ChelseaNoel, JacquelineKouzbari, M. ZahirBezamat, MarianaGranjeiro, José M.Antunes, Leonardo S.Antunes, Livia AzeredoAbreu, Fernanda Volpe deCosta, Marcelo C.Tannure, Patricia N.Seymen, FigenKoruyucu, MinePatir, AsliMereb, Juan C.Poletta, Fernando AdriánCastilla, Eduardo EnriqueOrioli, Ieda M.Marazita, Mary L.Vieira, Alexandre R.GeneticsOral healthPolymorphismCarieshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Our previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries. Methods: Seventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals. Results: Statistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence. Conclusions: Genetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored.Fil: Küchler, Erika C.. University of Pittsburgh; Estados UnidosFil: Deeley, Kathleen. University of Pittsburgh; Estados UnidosFil: Ho, Bao. University of Pittsburgh; Estados UnidosFil: Linkowski, Samantha. University of Pittsburgh; Estados UnidosFil: Meyer, Chelsea. University of Pittsburgh; Estados UnidosFil: Noel, Jacqueline. University of Pittsburgh; Estados UnidosFil: Kouzbari, M. Zahir. University of Pittsburgh; Estados UnidosFil: Bezamat, Mariana. University of Pittsburgh; Estados UnidosFil: Granjeiro, José M.. Universidade Federal Fluminense; BrasilFil: Antunes, Leonardo S.. Universidade Federal Fluminense; BrasilFil: Antunes, Livia Azeredo. Universidade Federal Fluminense; BrasilFil: Abreu, Fernanda Volpe de. Universidade Federal Fluminense; BrasilFil: Costa, Marcelo C.. Universidade Federal do Rio de Janeiro; BrasilFil: Tannure, Patricia N.. Veiga de Almeida; Brasil. Salgado de Oliveira University; BrasilFil: Seymen, Figen. Istanbul University; TurquíaFil: Koruyucu, Mine. Istanbul University; TurquíaFil: Patir, Asli. Istanbul University; TurquíaFil: Mereb, Juan C.. Estudio Colaborativo Latinoamericano de Malformaciones Congénitas; Argentina. Hospital de Área El Boóon; ArgentinaFil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina. Oswaldo Cruz Foundation; BrasilFil: Castilla, Eduardo Enrique. Oswaldo Cruz Foundation; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; ArgentinaFil: Orioli, Ieda M.. Universidade Federal do Rio de Janeiro; BrasilFil: Marazita, Mary L.. University of Pittsburgh; Estados UnidosFil: Vieira, Alexandre R.. University of Pittsburgh; Estados UnidosBioMed Central2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23882Küchler, Erika C.; Deeley, Kathleen; Ho, Bao; Linkowski, Samantha; Meyer, Chelsea; et al.; Genetic mapping of high caries experience on human chromosome 13; BioMed Central; BMC Medical Genetics; 14; 116; 11-2013; 1-101471-2350CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcmedgenet.biomedcentral.com/articles/10.1186/1471-2350-14-116info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2350-14-116info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:51Zoai:ri.conicet.gov.ar:11336/23882instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:51.996CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genetic mapping of high caries experience on human chromosome 13 |
title |
Genetic mapping of high caries experience on human chromosome 13 |
spellingShingle |
Genetic mapping of high caries experience on human chromosome 13 Küchler, Erika C. Genetics Oral health Polymorphism Caries |
title_short |
Genetic mapping of high caries experience on human chromosome 13 |
title_full |
Genetic mapping of high caries experience on human chromosome 13 |
title_fullStr |
Genetic mapping of high caries experience on human chromosome 13 |
title_full_unstemmed |
Genetic mapping of high caries experience on human chromosome 13 |
title_sort |
Genetic mapping of high caries experience on human chromosome 13 |
dc.creator.none.fl_str_mv |
Küchler, Erika C. Deeley, Kathleen Ho, Bao Linkowski, Samantha Meyer, Chelsea Noel, Jacqueline Kouzbari, M. Zahir Bezamat, Mariana Granjeiro, José M. Antunes, Leonardo S. Antunes, Livia Azeredo Abreu, Fernanda Volpe de Costa, Marcelo C. Tannure, Patricia N. Seymen, Figen Koruyucu, Mine Patir, Asli Mereb, Juan C. Poletta, Fernando Adrián Castilla, Eduardo Enrique Orioli, Ieda M. Marazita, Mary L. Vieira, Alexandre R. |
author |
Küchler, Erika C. |
author_facet |
Küchler, Erika C. Deeley, Kathleen Ho, Bao Linkowski, Samantha Meyer, Chelsea Noel, Jacqueline Kouzbari, M. Zahir Bezamat, Mariana Granjeiro, José M. Antunes, Leonardo S. Antunes, Livia Azeredo Abreu, Fernanda Volpe de Costa, Marcelo C. Tannure, Patricia N. Seymen, Figen Koruyucu, Mine Patir, Asli Mereb, Juan C. Poletta, Fernando Adrián Castilla, Eduardo Enrique Orioli, Ieda M. Marazita, Mary L. Vieira, Alexandre R. |
author_role |
author |
author2 |
Deeley, Kathleen Ho, Bao Linkowski, Samantha Meyer, Chelsea Noel, Jacqueline Kouzbari, M. Zahir Bezamat, Mariana Granjeiro, José M. Antunes, Leonardo S. Antunes, Livia Azeredo Abreu, Fernanda Volpe de Costa, Marcelo C. Tannure, Patricia N. Seymen, Figen Koruyucu, Mine Patir, Asli Mereb, Juan C. Poletta, Fernando Adrián Castilla, Eduardo Enrique Orioli, Ieda M. Marazita, Mary L. Vieira, Alexandre R. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Genetics Oral health Polymorphism Caries |
topic |
Genetics Oral health Polymorphism Caries |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Background: Our previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries. Methods: Seventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals. Results: Statistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence. Conclusions: Genetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored. Fil: Küchler, Erika C.. University of Pittsburgh; Estados Unidos Fil: Deeley, Kathleen. University of Pittsburgh; Estados Unidos Fil: Ho, Bao. University of Pittsburgh; Estados Unidos Fil: Linkowski, Samantha. University of Pittsburgh; Estados Unidos Fil: Meyer, Chelsea. University of Pittsburgh; Estados Unidos Fil: Noel, Jacqueline. University of Pittsburgh; Estados Unidos Fil: Kouzbari, M. Zahir. University of Pittsburgh; Estados Unidos Fil: Bezamat, Mariana. University of Pittsburgh; Estados Unidos Fil: Granjeiro, José M.. Universidade Federal Fluminense; Brasil Fil: Antunes, Leonardo S.. Universidade Federal Fluminense; Brasil Fil: Antunes, Livia Azeredo. Universidade Federal Fluminense; Brasil Fil: Abreu, Fernanda Volpe de. Universidade Federal Fluminense; Brasil Fil: Costa, Marcelo C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Tannure, Patricia N.. Veiga de Almeida; Brasil. Salgado de Oliveira University; Brasil Fil: Seymen, Figen. Istanbul University; Turquía Fil: Koruyucu, Mine. Istanbul University; Turquía Fil: Patir, Asli. Istanbul University; Turquía Fil: Mereb, Juan C.. Estudio Colaborativo Latinoamericano de Malformaciones Congénitas; Argentina. Hospital de Área El Boóon; Argentina Fil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina. Oswaldo Cruz Foundation; Brasil Fil: Castilla, Eduardo Enrique. Oswaldo Cruz Foundation; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina Fil: Orioli, Ieda M.. Universidade Federal do Rio de Janeiro; Brasil Fil: Marazita, Mary L.. University of Pittsburgh; Estados Unidos Fil: Vieira, Alexandre R.. University of Pittsburgh; Estados Unidos |
description |
Background: Our previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries. Methods: Seventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals. Results: Statistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence. Conclusions: Genetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/23882 Küchler, Erika C.; Deeley, Kathleen; Ho, Bao; Linkowski, Samantha; Meyer, Chelsea; et al.; Genetic mapping of high caries experience on human chromosome 13; BioMed Central; BMC Medical Genetics; 14; 116; 11-2013; 1-10 1471-2350 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/23882 |
identifier_str_mv |
Küchler, Erika C.; Deeley, Kathleen; Ho, Bao; Linkowski, Samantha; Meyer, Chelsea; et al.; Genetic mapping of high caries experience on human chromosome 13; BioMed Central; BMC Medical Genetics; 14; 116; 11-2013; 1-10 1471-2350 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://bmcmedgenet.biomedcentral.com/articles/10.1186/1471-2350-14-116 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2350-14-116 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |