Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides

Autores
Vázquez, Ana María; Gabri, Mariano Rolando; Hernández, Ana María; Alonso, Daniel Fernando; Beausoleil, Irene; Gomez, Daniel Eduardo; Pérez, Rolando
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We examined the antitumor effects of 1E10 monoclonal antibody, an anti-idiotypic IgG to an IgM monoclonal antibody, named P3, that reacts specifically with N-glycolyl-containing gangliosides and also recognizes antigens in human breast and melanoma tumors. Two murine tumor cell lines positive for the P3 antibody, F3II mammary carcinoma (BALB/c) and B16 melanoma (C57BL/6), were employed. In BALB/c mice, vaccination with several i.p. doses at 14-day intervals of 50 microgram of 1E10 coupled to keyhole limpet hemocyanin in Freund's adjuvant, significantly reduced s.c. tumor growth of F3II carcinoma cells and the number of spontaneous lung metastases. Also, the effect of 1E10 as a biological response modifier on tumor lung colonization was evaluated in C57BL/6 mice injected i.v. with B16 melanoma cells. Interestingly, i.v. administration of 10 microgram of uncoupled 1E10 antibody, 10-14 days after inoculation of B16 cells, dramatically reduced the number of experimental metastases in comparison with lungs from mice treated with an irrelevant IgG. The present data suggest that this 'non-internal image' anti-idiotypic monoclonal antibody may activate more than one mechanism of antitumor response against melanoma and mammary tumor cells.
Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Hernández, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Beausoleil, Irene. Centro de Inmunología Molecular; Cuba
Fil: Gomez, Daniel Eduardo. Centro de Inmunología Molecular; Cuba
Fil: Pérez, Rolando. Centro de Inmunología Molecular; Cuba
Materia
Immunotherapy
Metastasis
Murine Melanoma
Murine Mammary Cancer
Monoclonal Antibodies
Anti-Idiotypic Antibodies
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71974
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71974
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosidesVázquez, Ana MaríaGabri, Mariano RolandoHernández, Ana MaríaAlonso, Daniel FernandoBeausoleil, IreneGomez, Daniel EduardoPérez, RolandoImmunotherapyMetastasisMurine MelanomaMurine Mammary CancerMonoclonal AntibodiesAnti-Idiotypic Antibodieshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3We examined the antitumor effects of 1E10 monoclonal antibody, an anti-idiotypic IgG to an IgM monoclonal antibody, named P3, that reacts specifically with N-glycolyl-containing gangliosides and also recognizes antigens in human breast and melanoma tumors. Two murine tumor cell lines positive for the P3 antibody, F3II mammary carcinoma (BALB/c) and B16 melanoma (C57BL/6), were employed. In BALB/c mice, vaccination with several i.p. doses at 14-day intervals of 50 microgram of 1E10 coupled to keyhole limpet hemocyanin in Freund's adjuvant, significantly reduced s.c. tumor growth of F3II carcinoma cells and the number of spontaneous lung metastases. Also, the effect of 1E10 as a biological response modifier on tumor lung colonization was evaluated in C57BL/6 mice injected i.v. with B16 melanoma cells. Interestingly, i.v. administration of 10 microgram of uncoupled 1E10 antibody, 10-14 days after inoculation of B16 cells, dramatically reduced the number of experimental metastases in comparison with lungs from mice treated with an irrelevant IgG. The present data suggest that this 'non-internal image' anti-idiotypic monoclonal antibody may activate more than one mechanism of antitumor response against melanoma and mammary tumor cells.Fil: Vázquez, Ana María. Centro de Inmunología Molecular; CubaFil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; ArgentinaFil: Hernández, Ana María. Centro de Inmunología Molecular; CubaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; ArgentinaFil: Beausoleil, Irene. Centro de Inmunología Molecular; CubaFil: Gomez, Daniel Eduardo. Centro de Inmunología Molecular; CubaFil: Pérez, Rolando. Centro de Inmunología Molecular; CubaSpandidos Publications2000-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71974Vázquez, Ana María; Gabri, Mariano Rolando; Hernández, Ana María; Alonso, Daniel Fernando; Beausoleil, Irene; et al.; Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides; Spandidos Publications; Oncology Reports; 7; 7-2000; 751-7561021-335X1791-2431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/or/7/4/751info:eu-repo/semantics/altIdentifier/doi/10.3892/or.7.4.751info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:43Zoai:ri.conicet.gov.ar:11336/71974instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:43.694CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
title Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
spellingShingle Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
Vázquez, Ana María
Immunotherapy
Metastasis
Murine Melanoma
Murine Mammary Cancer
Monoclonal Antibodies
Anti-Idiotypic Antibodies
title_short Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
title_full Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
title_fullStr Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
title_full_unstemmed Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
title_sort Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides
dc.creator.none.fl_str_mv Vázquez, Ana María
Gabri, Mariano Rolando
Hernández, Ana María
Alonso, Daniel Fernando
Beausoleil, Irene
Gomez, Daniel Eduardo
Pérez, Rolando
author Vázquez, Ana María
author_facet Vázquez, Ana María
Gabri, Mariano Rolando
Hernández, Ana María
Alonso, Daniel Fernando
Beausoleil, Irene
Gomez, Daniel Eduardo
Pérez, Rolando
author_role author
author2 Gabri, Mariano Rolando
Hernández, Ana María
Alonso, Daniel Fernando
Beausoleil, Irene
Gomez, Daniel Eduardo
Pérez, Rolando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunotherapy
Metastasis
Murine Melanoma
Murine Mammary Cancer
Monoclonal Antibodies
Anti-Idiotypic Antibodies
topic Immunotherapy
Metastasis
Murine Melanoma
Murine Mammary Cancer
Monoclonal Antibodies
Anti-Idiotypic Antibodies
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We examined the antitumor effects of 1E10 monoclonal antibody, an anti-idiotypic IgG to an IgM monoclonal antibody, named P3, that reacts specifically with N-glycolyl-containing gangliosides and also recognizes antigens in human breast and melanoma tumors. Two murine tumor cell lines positive for the P3 antibody, F3II mammary carcinoma (BALB/c) and B16 melanoma (C57BL/6), were employed. In BALB/c mice, vaccination with several i.p. doses at 14-day intervals of 50 microgram of 1E10 coupled to keyhole limpet hemocyanin in Freund's adjuvant, significantly reduced s.c. tumor growth of F3II carcinoma cells and the number of spontaneous lung metastases. Also, the effect of 1E10 as a biological response modifier on tumor lung colonization was evaluated in C57BL/6 mice injected i.v. with B16 melanoma cells. Interestingly, i.v. administration of 10 microgram of uncoupled 1E10 antibody, 10-14 days after inoculation of B16 cells, dramatically reduced the number of experimental metastases in comparison with lungs from mice treated with an irrelevant IgG. The present data suggest that this 'non-internal image' anti-idiotypic monoclonal antibody may activate more than one mechanism of antitumor response against melanoma and mammary tumor cells.
Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Hernández, Ana María. Centro de Inmunología Molecular; Cuba
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Beausoleil, Irene. Centro de Inmunología Molecular; Cuba
Fil: Gomez, Daniel Eduardo. Centro de Inmunología Molecular; Cuba
Fil: Pérez, Rolando. Centro de Inmunología Molecular; Cuba
description We examined the antitumor effects of 1E10 monoclonal antibody, an anti-idiotypic IgG to an IgM monoclonal antibody, named P3, that reacts specifically with N-glycolyl-containing gangliosides and also recognizes antigens in human breast and melanoma tumors. Two murine tumor cell lines positive for the P3 antibody, F3II mammary carcinoma (BALB/c) and B16 melanoma (C57BL/6), were employed. In BALB/c mice, vaccination with several i.p. doses at 14-day intervals of 50 microgram of 1E10 coupled to keyhole limpet hemocyanin in Freund's adjuvant, significantly reduced s.c. tumor growth of F3II carcinoma cells and the number of spontaneous lung metastases. Also, the effect of 1E10 as a biological response modifier on tumor lung colonization was evaluated in C57BL/6 mice injected i.v. with B16 melanoma cells. Interestingly, i.v. administration of 10 microgram of uncoupled 1E10 antibody, 10-14 days after inoculation of B16 cells, dramatically reduced the number of experimental metastases in comparison with lungs from mice treated with an irrelevant IgG. The present data suggest that this 'non-internal image' anti-idiotypic monoclonal antibody may activate more than one mechanism of antitumor response against melanoma and mammary tumor cells.
publishDate 2000
dc.date.none.fl_str_mv 2000-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71974
Vázquez, Ana María; Gabri, Mariano Rolando; Hernández, Ana María; Alonso, Daniel Fernando; Beausoleil, Irene; et al.; Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides; Spandidos Publications; Oncology Reports; 7; 7-2000; 751-756
1021-335X
1791-2431
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71974
identifier_str_mv Vázquez, Ana María; Gabri, Mariano Rolando; Hernández, Ana María; Alonso, Daniel Fernando; Beausoleil, Irene; et al.; Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides; Spandidos Publications; Oncology Reports; 7; 7-2000; 751-756
1021-335X
1791-2431
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/or/7/4/751
info:eu-repo/semantics/altIdentifier/doi/10.3892/or.7.4.751
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spandidos Publications
publisher.none.fl_str_mv Spandidos Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980665687015424
score 12.993085

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