Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina

Autores
Amavet, Patricia Susana; Rosso, Esteban; Markariani, Rosa; Piña, Carlos Ignacio
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Detecting multiple paternity in wild populations of the broad-snouted caiman (Caiman latirostris) has important implications for conservation efforts. We have applied microsatellite markers to examine genetic variation in C. latirostris and also have provided the first data concerning detection of multiple paternity in wild populations of this species. Blood samples from four nest-guarding C. latirostris females and their hatchlings were obtained from Santa Fe Province, Argentina. Amplified products were analyzed by electrophoresis on 10% polyacrylamide gels and visualized with silver staining. Four out of the eight markers tested reliably amplified and yielded useful data. Using polyacrylamide gels with silver staining provides high enough resolution to obtain individual genotypes. In order to assess the presence or absence of more than two parents in each nest, we used the single locus Minimum Method, and applied Cervus 3.0 and Gerud 2.0 software in parentage analyses. Our results indicate more than one father in at least two families. This behavior could be the consequence of high habitat variability in the area where our population was sampled. The ability to understand mating systems is important for maintaining viable populations of exploited taxa like C. latirostris.
Fil: Amavet, Patricia Susana. Universidad Autonoma de Entre Rios; Argentina. Proyecto Yacaré; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina
Fil: Rosso, Esteban. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina
Fil: Markariani, Rosa. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina
Fil: Piña, Carlos Ignacio. Proyecto Yacare; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Materia
Caiman Latirostris
Mating System
Multiple Paternity
Molecular Markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80901
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, ArgentinaAmavet, Patricia SusanaRosso, EstebanMarkariani, RosaPiña, Carlos IgnacioCaiman LatirostrisMating SystemMultiple PaternityMolecular Markershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Detecting multiple paternity in wild populations of the broad-snouted caiman (Caiman latirostris) has important implications for conservation efforts. We have applied microsatellite markers to examine genetic variation in C. latirostris and also have provided the first data concerning detection of multiple paternity in wild populations of this species. Blood samples from four nest-guarding C. latirostris females and their hatchlings were obtained from Santa Fe Province, Argentina. Amplified products were analyzed by electrophoresis on 10% polyacrylamide gels and visualized with silver staining. Four out of the eight markers tested reliably amplified and yielded useful data. Using polyacrylamide gels with silver staining provides high enough resolution to obtain individual genotypes. In order to assess the presence or absence of more than two parents in each nest, we used the single locus Minimum Method, and applied Cervus 3.0 and Gerud 2.0 software in parentage analyses. Our results indicate more than one father in at least two families. This behavior could be the consequence of high habitat variability in the area where our population was sampled. The ability to understand mating systems is important for maintaining viable populations of exploited taxa like C. latirostris.Fil: Amavet, Patricia Susana. Universidad Autonoma de Entre Rios; Argentina. Proyecto Yacaré; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; ArgentinaFil: Rosso, Esteban. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; ArgentinaFil: Markariani, Rosa. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; ArgentinaFil: Piña, Carlos Ignacio. Proyecto Yacare; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaWiley-liss, Div John Wiley & Sons Inc2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80901Amavet, Patricia Susana; Rosso, Esteban; Markariani, Rosa; Piña, Carlos Ignacio; Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina; Wiley-liss, Div John Wiley & Sons Inc; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 309A; 10; 12-2008; 637-6421932-5223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jez.496info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.496info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:22Zoai:ri.conicet.gov.ar:11336/80901instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:22.596CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
title Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
spellingShingle Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
Amavet, Patricia Susana
Caiman Latirostris
Mating System
Multiple Paternity
Molecular Markers
title_short Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
title_full Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
title_fullStr Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
title_full_unstemmed Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
title_sort Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina
dc.creator.none.fl_str_mv Amavet, Patricia Susana
Rosso, Esteban
Markariani, Rosa
Piña, Carlos Ignacio
author Amavet, Patricia Susana
author_facet Amavet, Patricia Susana
Rosso, Esteban
Markariani, Rosa
Piña, Carlos Ignacio
author_role author
author2 Rosso, Esteban
Markariani, Rosa
Piña, Carlos Ignacio
author2_role author
author
author
dc.subject.none.fl_str_mv Caiman Latirostris
Mating System
Multiple Paternity
Molecular Markers
topic Caiman Latirostris
Mating System
Multiple Paternity
Molecular Markers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Detecting multiple paternity in wild populations of the broad-snouted caiman (Caiman latirostris) has important implications for conservation efforts. We have applied microsatellite markers to examine genetic variation in C. latirostris and also have provided the first data concerning detection of multiple paternity in wild populations of this species. Blood samples from four nest-guarding C. latirostris females and their hatchlings were obtained from Santa Fe Province, Argentina. Amplified products were analyzed by electrophoresis on 10% polyacrylamide gels and visualized with silver staining. Four out of the eight markers tested reliably amplified and yielded useful data. Using polyacrylamide gels with silver staining provides high enough resolution to obtain individual genotypes. In order to assess the presence or absence of more than two parents in each nest, we used the single locus Minimum Method, and applied Cervus 3.0 and Gerud 2.0 software in parentage analyses. Our results indicate more than one father in at least two families. This behavior could be the consequence of high habitat variability in the area where our population was sampled. The ability to understand mating systems is important for maintaining viable populations of exploited taxa like C. latirostris.
Fil: Amavet, Patricia Susana. Universidad Autonoma de Entre Rios; Argentina. Proyecto Yacaré; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina
Fil: Rosso, Esteban. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina
Fil: Markariani, Rosa. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina
Fil: Piña, Carlos Ignacio. Proyecto Yacare; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
description Detecting multiple paternity in wild populations of the broad-snouted caiman (Caiman latirostris) has important implications for conservation efforts. We have applied microsatellite markers to examine genetic variation in C. latirostris and also have provided the first data concerning detection of multiple paternity in wild populations of this species. Blood samples from four nest-guarding C. latirostris females and their hatchlings were obtained from Santa Fe Province, Argentina. Amplified products were analyzed by electrophoresis on 10% polyacrylamide gels and visualized with silver staining. Four out of the eight markers tested reliably amplified and yielded useful data. Using polyacrylamide gels with silver staining provides high enough resolution to obtain individual genotypes. In order to assess the presence or absence of more than two parents in each nest, we used the single locus Minimum Method, and applied Cervus 3.0 and Gerud 2.0 software in parentage analyses. Our results indicate more than one father in at least two families. This behavior could be the consequence of high habitat variability in the area where our population was sampled. The ability to understand mating systems is important for maintaining viable populations of exploited taxa like C. latirostris.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80901
Amavet, Patricia Susana; Rosso, Esteban; Markariani, Rosa; Piña, Carlos Ignacio; Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina; Wiley-liss, Div John Wiley & Sons Inc; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 309A; 10; 12-2008; 637-642
1932-5223
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80901
identifier_str_mv Amavet, Patricia Susana; Rosso, Esteban; Markariani, Rosa; Piña, Carlos Ignacio; Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina; Wiley-liss, Div John Wiley & Sons Inc; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 309A; 10; 12-2008; 637-642
1932-5223
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.496
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.496
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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