Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics

Autores
Dib, Julian Rafael; Weiss, Annika; Neumann, Anna; Ordoñez, Omar Federico; Estévez, Maria Cristina; Farias, Maria Eugenia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High altitude Andean lakes are placed in Puna desert over 4400 above sea level. Completely isolated, they are exposed to extreme environmental factors like high levels of salinity, UV radiation and heavy metals and low concentrations of phosphorus. Nevertheless, they are the habitat of enormous populations of three flamingo species that migrate among these Lakes. Previous reports have determined that bacteria isolated from these environments present high levels of resistance to antibiotics. The aim of this work was to determine the diversity of antibiotic resistant bacteria in water from Andean Lakes and their connection with flamingo enteric biota. Bacteria from water and birds faeces from high altitude Lakes: Laguna (L.) Aparejos, L. Negra, L. Vilama and L. Azul (all are located between 4,200 and 4,600 m altitude) were isolated by plating in five different Antibiotics (ampicillin, 100 microg ml(-1); chloramphenicol, 170 microg ml(-1); colistin , 20 microg ml(-1); erythromycin, 50 microg ml(-1) and tetracycline 50 microg ml(-1)). 56 bacteria were isolated and identified by 16 S rDNA sequencing. Antibiotic resistance profiles of isolated bacteria were determined for 22 different antibiotics. All identified bacteria were able to growth in multiple ATBs. Colistin, ceftazidime, ampicillin/sulbactam, cefotaxime, cefepime, cefalotin, ampicillin and erythromycin were the most distributed resistances among the 56 tested bacteria. The current results demonstrated that antibiotic resistance was abundant and diverse in high altitude Lakes. Also the present article indicates some useful patents regarding the isolation of bacteria able to grow in the present of antibiotics.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Weiss, Annika. Technishe Universitat Berlin; Alemania
Fil: Neumann, Anna. Technishe Universitat Berlin; Alemania
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Andean Lakes Microbiology
Antibiotics Resistance
Extreme Environments
Water Birds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43646
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43646
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of AntibioticsDib, Julian RafaelWeiss, AnnikaNeumann, AnnaOrdoñez, Omar FedericoEstévez, Maria CristinaFarias, Maria EugeniaAndean Lakes MicrobiologyAntibiotics ResistanceExtreme EnvironmentsWater Birdshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High altitude Andean lakes are placed in Puna desert over 4400 above sea level. Completely isolated, they are exposed to extreme environmental factors like high levels of salinity, UV radiation and heavy metals and low concentrations of phosphorus. Nevertheless, they are the habitat of enormous populations of three flamingo species that migrate among these Lakes. Previous reports have determined that bacteria isolated from these environments present high levels of resistance to antibiotics. The aim of this work was to determine the diversity of antibiotic resistant bacteria in water from Andean Lakes and their connection with flamingo enteric biota. Bacteria from water and birds faeces from high altitude Lakes: Laguna (L.) Aparejos, L. Negra, L. Vilama and L. Azul (all are located between 4,200 and 4,600 m altitude) were isolated by plating in five different Antibiotics (ampicillin, 100 microg ml(-1); chloramphenicol, 170 microg ml(-1); colistin , 20 microg ml(-1); erythromycin, 50 microg ml(-1) and tetracycline 50 microg ml(-1)). 56 bacteria were isolated and identified by 16 S rDNA sequencing. Antibiotic resistance profiles of isolated bacteria were determined for 22 different antibiotics. All identified bacteria were able to growth in multiple ATBs. Colistin, ceftazidime, ampicillin/sulbactam, cefotaxime, cefepime, cefalotin, ampicillin and erythromycin were the most distributed resistances among the 56 tested bacteria. The current results demonstrated that antibiotic resistance was abundant and diverse in high altitude Lakes. Also the present article indicates some useful patents regarding the isolation of bacteria able to grow in the present of antibiotics.Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Weiss, Annika. Technishe Universitat Berlin; AlemaniaFil: Neumann, Anna. Technishe Universitat Berlin; AlemaniaFil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaBentham Science Publishers2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43646Dib, Julian Rafael; Weiss, Annika; Neumann, Anna; Ordoñez, Omar Federico; Estévez, Maria Cristina; et al.; Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics; Bentham Science Publishers; Recent Patents on Anti-Infective Drug Discovery; 4; 1; 1-2009; 66-761574-891X2212-4071CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.recentpatentsonantiinfectivedrugdiscovery.com/articles/83792/-isolation-of-bacteria-from-remote-high-altitude-andean-lakes-able-to-grow-in-the-presence-of-antibioticsinfo:eu-repo/semantics/altIdentifier/doi/10.2174/157489109787236300info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/83792/articleinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:23Zoai:ri.conicet.gov.ar:11336/43646instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:23.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
title Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
spellingShingle Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
Dib, Julian Rafael
Andean Lakes Microbiology
Antibiotics Resistance
Extreme Environments
Water Birds
title_short Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
title_full Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
title_fullStr Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
title_full_unstemmed Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
title_sort Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics
dc.creator.none.fl_str_mv Dib, Julian Rafael
Weiss, Annika
Neumann, Anna
Ordoñez, Omar Federico
Estévez, Maria Cristina
Farias, Maria Eugenia
author Dib, Julian Rafael
author_facet Dib, Julian Rafael
Weiss, Annika
Neumann, Anna
Ordoñez, Omar Federico
Estévez, Maria Cristina
Farias, Maria Eugenia
author_role author
author2 Weiss, Annika
Neumann, Anna
Ordoñez, Omar Federico
Estévez, Maria Cristina
Farias, Maria Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Andean Lakes Microbiology
Antibiotics Resistance
Extreme Environments
Water Birds
topic Andean Lakes Microbiology
Antibiotics Resistance
Extreme Environments
Water Birds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High altitude Andean lakes are placed in Puna desert over 4400 above sea level. Completely isolated, they are exposed to extreme environmental factors like high levels of salinity, UV radiation and heavy metals and low concentrations of phosphorus. Nevertheless, they are the habitat of enormous populations of three flamingo species that migrate among these Lakes. Previous reports have determined that bacteria isolated from these environments present high levels of resistance to antibiotics. The aim of this work was to determine the diversity of antibiotic resistant bacteria in water from Andean Lakes and their connection with flamingo enteric biota. Bacteria from water and birds faeces from high altitude Lakes: Laguna (L.) Aparejos, L. Negra, L. Vilama and L. Azul (all are located between 4,200 and 4,600 m altitude) were isolated by plating in five different Antibiotics (ampicillin, 100 microg ml(-1); chloramphenicol, 170 microg ml(-1); colistin , 20 microg ml(-1); erythromycin, 50 microg ml(-1) and tetracycline 50 microg ml(-1)). 56 bacteria were isolated and identified by 16 S rDNA sequencing. Antibiotic resistance profiles of isolated bacteria were determined for 22 different antibiotics. All identified bacteria were able to growth in multiple ATBs. Colistin, ceftazidime, ampicillin/sulbactam, cefotaxime, cefepime, cefalotin, ampicillin and erythromycin were the most distributed resistances among the 56 tested bacteria. The current results demonstrated that antibiotic resistance was abundant and diverse in high altitude Lakes. Also the present article indicates some useful patents regarding the isolation of bacteria able to grow in the present of antibiotics.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Weiss, Annika. Technishe Universitat Berlin; Alemania
Fil: Neumann, Anna. Technishe Universitat Berlin; Alemania
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description High altitude Andean lakes are placed in Puna desert over 4400 above sea level. Completely isolated, they are exposed to extreme environmental factors like high levels of salinity, UV radiation and heavy metals and low concentrations of phosphorus. Nevertheless, they are the habitat of enormous populations of three flamingo species that migrate among these Lakes. Previous reports have determined that bacteria isolated from these environments present high levels of resistance to antibiotics. The aim of this work was to determine the diversity of antibiotic resistant bacteria in water from Andean Lakes and their connection with flamingo enteric biota. Bacteria from water and birds faeces from high altitude Lakes: Laguna (L.) Aparejos, L. Negra, L. Vilama and L. Azul (all are located between 4,200 and 4,600 m altitude) were isolated by plating in five different Antibiotics (ampicillin, 100 microg ml(-1); chloramphenicol, 170 microg ml(-1); colistin , 20 microg ml(-1); erythromycin, 50 microg ml(-1) and tetracycline 50 microg ml(-1)). 56 bacteria were isolated and identified by 16 S rDNA sequencing. Antibiotic resistance profiles of isolated bacteria were determined for 22 different antibiotics. All identified bacteria were able to growth in multiple ATBs. Colistin, ceftazidime, ampicillin/sulbactam, cefotaxime, cefepime, cefalotin, ampicillin and erythromycin were the most distributed resistances among the 56 tested bacteria. The current results demonstrated that antibiotic resistance was abundant and diverse in high altitude Lakes. Also the present article indicates some useful patents regarding the isolation of bacteria able to grow in the present of antibiotics.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43646
Dib, Julian Rafael; Weiss, Annika; Neumann, Anna; Ordoñez, Omar Federico; Estévez, Maria Cristina; et al.; Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics; Bentham Science Publishers; Recent Patents on Anti-Infective Drug Discovery; 4; 1; 1-2009; 66-76
1574-891X
2212-4071
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43646
identifier_str_mv Dib, Julian Rafael; Weiss, Annika; Neumann, Anna; Ordoñez, Omar Federico; Estévez, Maria Cristina; et al.; Isolation of Bacteria from Remote High Altitude Andean Lakes Able to Grow in the Presence of Antibiotics; Bentham Science Publishers; Recent Patents on Anti-Infective Drug Discovery; 4; 1; 1-2009; 66-76
1574-891X
2212-4071
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.recentpatentsonantiinfectivedrugdiscovery.com/articles/83792/-isolation-of-bacteria-from-remote-high-altitude-andean-lakes-able-to-grow-in-the-presence-of-antibiotics
info:eu-repo/semantics/altIdentifier/doi/10.2174/157489109787236300
info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/83792/article
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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