Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties

Autores
Abraham, Gustavo Abel; De Queiroz, Alvaro A.A; Román, Julio San
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A method has been developed in which a layer of p-aminosalicylic acid (4-amino-2-hydroxybenzoic acid) (PAS), a water soluble pharmaceutical compound of the nonsteroidal anti-inflammatory drug (NSAID) class with antiaggregant platelet activity, is covalently immobilized onto a segmented polyurethane, Biospan™ (SPU) surface. Thus, SPU surfaces were modified by grafting of hexamethylenediisocyanate, and the free isocyanate remaining on the SPU surface were then coupled through a condensation reaction to amine groups of p-aminosalicylic acid. The bonding of PAS from aqueous solution onto SPU surface was studied by ATR-FTIR, UV and fluorescence spectroscopy. Plateau levels of coupled PAS were reached within 1.2μg/cm2 using PAS solution concentrations of 1mg/ml. The surface wettability of the polymeric films measured by contact angle indicate that the introduction of the PAS turns the surface more hydrophilic (θwater=43.1°±2.1°) relatively to the original SPU films (θwater=70.3°±1.9°). The in vitro albumin (BSA) adsorption shows that the PAS-SPU films adsorb more BSA (250/μgmm2) than the original SPU (112μg/mm2). Thrombogenicity was assessed by measuring the thrombus formation and platelet adhesion of the SPU containing PAS relatively to nonmodified SPU surfaces. The polymeric surfaces with immobilized PAS had better nonthrombogenic characteristics as indicated by the low platelet adhesion, high adsorption of albumin relatively to fibrinogen and low thrombus formation, making them potentially good candidates for biomedical applications. © 2002 Elsevier Science Ltd. All rights reserved.
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: De Queiroz, Alvaro A.A. Escola Federal de Engenharia de Itajubá; Brasil
Fil: Román, Julio San. Consejo Superior de Investigaciones Científicas; España
Materia
Albumin
Biospan&Trade;
P-Aminobenzoic Acid
Segmented Polyurethanes
Thrombogenicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54233
Acceder
id CONICETDig_005a24a39ecfbfe9bf621e52eb140039
oai_identifier_str oai:ri.conicet.gov.ar:11336/54233
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility propertiesAbraham, Gustavo AbelDe Queiroz, Alvaro A.ARomán, Julio SanAlbuminBiospan&Trade;P-Aminobenzoic AcidSegmented PolyurethanesThrombogenicityhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A method has been developed in which a layer of p-aminosalicylic acid (4-amino-2-hydroxybenzoic acid) (PAS), a water soluble pharmaceutical compound of the nonsteroidal anti-inflammatory drug (NSAID) class with antiaggregant platelet activity, is covalently immobilized onto a segmented polyurethane, Biospan™ (SPU) surface. Thus, SPU surfaces were modified by grafting of hexamethylenediisocyanate, and the free isocyanate remaining on the SPU surface were then coupled through a condensation reaction to amine groups of p-aminosalicylic acid. The bonding of PAS from aqueous solution onto SPU surface was studied by ATR-FTIR, UV and fluorescence spectroscopy. Plateau levels of coupled PAS were reached within 1.2μg/cm2 using PAS solution concentrations of 1mg/ml. The surface wettability of the polymeric films measured by contact angle indicate that the introduction of the PAS turns the surface more hydrophilic (θwater=43.1°±2.1°) relatively to the original SPU films (θwater=70.3°±1.9°). The in vitro albumin (BSA) adsorption shows that the PAS-SPU films adsorb more BSA (250/μgmm2) than the original SPU (112μg/mm2). Thrombogenicity was assessed by measuring the thrombus formation and platelet adhesion of the SPU containing PAS relatively to nonmodified SPU surfaces. The polymeric surfaces with immobilized PAS had better nonthrombogenic characteristics as indicated by the low platelet adhesion, high adsorption of albumin relatively to fibrinogen and low thrombus formation, making them potentially good candidates for biomedical applications. © 2002 Elsevier Science Ltd. All rights reserved.Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: De Queiroz, Alvaro A.A. Escola Federal de Engenharia de Itajubá; BrasilFil: Román, Julio San. Consejo Superior de Investigaciones Científicas; EspañaElsevier2002-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54233Abraham, Gustavo Abel; De Queiroz, Alvaro A.A; Román, Julio San; Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties; Elsevier; Biomaterials; 23; 7; 4-2002; 1625-16380142-9612CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0142-9612(01)00289-7info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0142961201002897info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:29Zoai:ri.conicet.gov.ar:11336/54233instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:29.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
title Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
spellingShingle Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
Abraham, Gustavo Abel
Albumin
Biospan&Trade;
P-Aminobenzoic Acid
Segmented Polyurethanes
Thrombogenicity
title_short Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
title_full Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
title_fullStr Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
title_full_unstemmed Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
title_sort Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties
dc.creator.none.fl_str_mv Abraham, Gustavo Abel
De Queiroz, Alvaro A.A
Román, Julio San
author Abraham, Gustavo Abel
author_facet Abraham, Gustavo Abel
De Queiroz, Alvaro A.A
Román, Julio San
author_role author
author2 De Queiroz, Alvaro A.A
Román, Julio San
author2_role author
author
dc.subject.none.fl_str_mv Albumin
Biospan&Trade;
P-Aminobenzoic Acid
Segmented Polyurethanes
Thrombogenicity
topic Albumin
Biospan&Trade;
P-Aminobenzoic Acid
Segmented Polyurethanes
Thrombogenicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A method has been developed in which a layer of p-aminosalicylic acid (4-amino-2-hydroxybenzoic acid) (PAS), a water soluble pharmaceutical compound of the nonsteroidal anti-inflammatory drug (NSAID) class with antiaggregant platelet activity, is covalently immobilized onto a segmented polyurethane, Biospan™ (SPU) surface. Thus, SPU surfaces were modified by grafting of hexamethylenediisocyanate, and the free isocyanate remaining on the SPU surface were then coupled through a condensation reaction to amine groups of p-aminosalicylic acid. The bonding of PAS from aqueous solution onto SPU surface was studied by ATR-FTIR, UV and fluorescence spectroscopy. Plateau levels of coupled PAS were reached within 1.2μg/cm2 using PAS solution concentrations of 1mg/ml. The surface wettability of the polymeric films measured by contact angle indicate that the introduction of the PAS turns the surface more hydrophilic (θwater=43.1°±2.1°) relatively to the original SPU films (θwater=70.3°±1.9°). The in vitro albumin (BSA) adsorption shows that the PAS-SPU films adsorb more BSA (250/μgmm2) than the original SPU (112μg/mm2). Thrombogenicity was assessed by measuring the thrombus formation and platelet adhesion of the SPU containing PAS relatively to nonmodified SPU surfaces. The polymeric surfaces with immobilized PAS had better nonthrombogenic characteristics as indicated by the low platelet adhesion, high adsorption of albumin relatively to fibrinogen and low thrombus formation, making them potentially good candidates for biomedical applications. © 2002 Elsevier Science Ltd. All rights reserved.
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: De Queiroz, Alvaro A.A. Escola Federal de Engenharia de Itajubá; Brasil
Fil: Román, Julio San. Consejo Superior de Investigaciones Científicas; España
description A method has been developed in which a layer of p-aminosalicylic acid (4-amino-2-hydroxybenzoic acid) (PAS), a water soluble pharmaceutical compound of the nonsteroidal anti-inflammatory drug (NSAID) class with antiaggregant platelet activity, is covalently immobilized onto a segmented polyurethane, Biospan™ (SPU) surface. Thus, SPU surfaces were modified by grafting of hexamethylenediisocyanate, and the free isocyanate remaining on the SPU surface were then coupled through a condensation reaction to amine groups of p-aminosalicylic acid. The bonding of PAS from aqueous solution onto SPU surface was studied by ATR-FTIR, UV and fluorescence spectroscopy. Plateau levels of coupled PAS were reached within 1.2μg/cm2 using PAS solution concentrations of 1mg/ml. The surface wettability of the polymeric films measured by contact angle indicate that the introduction of the PAS turns the surface more hydrophilic (θwater=43.1°±2.1°) relatively to the original SPU films (θwater=70.3°±1.9°). The in vitro albumin (BSA) adsorption shows that the PAS-SPU films adsorb more BSA (250/μgmm2) than the original SPU (112μg/mm2). Thrombogenicity was assessed by measuring the thrombus formation and platelet adhesion of the SPU containing PAS relatively to nonmodified SPU surfaces. The polymeric surfaces with immobilized PAS had better nonthrombogenic characteristics as indicated by the low platelet adhesion, high adsorption of albumin relatively to fibrinogen and low thrombus formation, making them potentially good candidates for biomedical applications. © 2002 Elsevier Science Ltd. All rights reserved.
publishDate 2002
dc.date.none.fl_str_mv 2002-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54233
Abraham, Gustavo Abel; De Queiroz, Alvaro A.A; Román, Julio San; Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties; Elsevier; Biomaterials; 23; 7; 4-2002; 1625-1638
0142-9612
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54233
identifier_str_mv Abraham, Gustavo Abel; De Queiroz, Alvaro A.A; Román, Julio San; Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties; Elsevier; Biomaterials; 23; 7; 4-2002; 1625-1638
0142-9612
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0142-9612(01)00289-7
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0142961201002897
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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