Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America

Autores
Castañares, Eliana; Dinolfo, María Inés; Del Ponte, E. M.; Pan, D.; Stenglein, Sebastián
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Members of the Fusarium graminearum species complex (FGSC), such as F. graminearum and F. asiaticum, are the main cause of fusarium head blight (FHB) of wheat and barley worldwide. In this study, 117 FGSC isolates obtained from commercial barley grain produced in Argentina (n = 43 isolates), Brazil (n = 35), and Uruguay (n = 39) were identified to species and trichothecene genotypes, and analysed using amplified fragment length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers. In addition, reductase (RED) and trichothecene 3-Oacetyltransferase (Tri101) were sequenced for a subset of 24 isolates. The majority of the isolates (n = 103) were identified as F. graminearum, which was the only species found in Argentina. In Uruguay, only one F. cortaderiae isolate was found among F. graminearum isolates. In Brazil, F. graminearum also dominated the collection (22/35), followed by F. meridionale (8/35), F. asiaticum (2/35), F. cortaderiae (2/35) and F. austroamericanum (1/35). Species were structured by trichothecene genotype: all F. graminearum were of the 15-acetyldeoxynivalenol (ADON), F. meridionale, F. asiaticum and F. cortaderiae were of the nivalenol (NIV), and F. austroamericanum was of the 3 ADON genotype. Both AFLP and SRAP data showed high levels of genetic variability, which was higher within than among countries. Isolates were not structured by country of origin. SRAP analysis grouped F. graminearum in a separate cluster from the other species within the complex. However, AFLP analysis failed to resolve the species into distinct clades with partial clustering of F. meridionale, F. austroamericanum, F. asiaticum and F graminearum isolates.
Materia
Agronomía, reproducción y protección de plantas
AFLP
FGSC
Hordeum vulgare
SRAP
trichothecene genotype
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/6287

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network_name_str CIC Digital (CICBA)
spelling Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South AmericaCastañares, ElianaDinolfo, María InésDel Ponte, E. M.Pan, D.Stenglein, SebastiánAgronomía, reproducción y protección de plantasAFLPFGSCHordeum vulgareSRAPtrichothecene genotypeMembers of the Fusarium graminearum species complex (FGSC), such as F. graminearum and F. asiaticum, are the main cause of fusarium head blight (FHB) of wheat and barley worldwide. In this study, 117 FGSC isolates obtained from commercial barley grain produced in Argentina (n = 43 isolates), Brazil (n = 35), and Uruguay (n = 39) were identified to species and trichothecene genotypes, and analysed using amplified fragment length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers. In addition, reductase (RED) and trichothecene 3-Oacetyltransferase (Tri101) were sequenced for a subset of 24 isolates. The majority of the isolates (n = 103) were identified as F. graminearum, which was the only species found in Argentina. In Uruguay, only one F. cortaderiae isolate was found among F. graminearum isolates. In Brazil, F. graminearum also dominated the collection (22/35), followed by F. meridionale (8/35), F. asiaticum (2/35), F. cortaderiae (2/35) and F. austroamericanum (1/35). Species were structured by trichothecene genotype: all F. graminearum were of the 15-acetyldeoxynivalenol (ADON), F. meridionale, F. asiaticum and F. cortaderiae were of the nivalenol (NIV), and F. austroamericanum was of the 3 ADON genotype. Both AFLP and SRAP data showed high levels of genetic variability, which was higher within than among countries. Isolates were not structured by country of origin. SRAP analysis grouped F. graminearum in a separate cluster from the other species within the complex. However, AFLP analysis failed to resolve the species into distinct clades with partial clustering of F. meridionale, F. austroamericanum, F. asiaticum and F graminearum isolates.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/6287enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.12470info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:43:27Zoai:digital.cic.gba.gob.ar:11746/6287Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:43:27.542CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
title Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
spellingShingle Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
Castañares, Eliana
Agronomía, reproducción y protección de plantas
AFLP
FGSC
Hordeum vulgare
SRAP
trichothecene genotype
title_short Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
title_full Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
title_fullStr Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
title_full_unstemmed Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
title_sort Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America
dc.creator.none.fl_str_mv Castañares, Eliana
Dinolfo, María Inés
Del Ponte, E. M.
Pan, D.
Stenglein, Sebastián
author Castañares, Eliana
author_facet Castañares, Eliana
Dinolfo, María Inés
Del Ponte, E. M.
Pan, D.
Stenglein, Sebastián
author_role author
author2 Dinolfo, María Inés
Del Ponte, E. M.
Pan, D.
Stenglein, Sebastián
author2_role author
author
author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
AFLP
FGSC
Hordeum vulgare
SRAP
trichothecene genotype
topic Agronomía, reproducción y protección de plantas
AFLP
FGSC
Hordeum vulgare
SRAP
trichothecene genotype
dc.description.none.fl_txt_mv Members of the Fusarium graminearum species complex (FGSC), such as F. graminearum and F. asiaticum, are the main cause of fusarium head blight (FHB) of wheat and barley worldwide. In this study, 117 FGSC isolates obtained from commercial barley grain produced in Argentina (n = 43 isolates), Brazil (n = 35), and Uruguay (n = 39) were identified to species and trichothecene genotypes, and analysed using amplified fragment length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers. In addition, reductase (RED) and trichothecene 3-Oacetyltransferase (Tri101) were sequenced for a subset of 24 isolates. The majority of the isolates (n = 103) were identified as F. graminearum, which was the only species found in Argentina. In Uruguay, only one F. cortaderiae isolate was found among F. graminearum isolates. In Brazil, F. graminearum also dominated the collection (22/35), followed by F. meridionale (8/35), F. asiaticum (2/35), F. cortaderiae (2/35) and F. austroamericanum (1/35). Species were structured by trichothecene genotype: all F. graminearum were of the 15-acetyldeoxynivalenol (ADON), F. meridionale, F. asiaticum and F. cortaderiae were of the nivalenol (NIV), and F. austroamericanum was of the 3 ADON genotype. Both AFLP and SRAP data showed high levels of genetic variability, which was higher within than among countries. Isolates were not structured by country of origin. SRAP analysis grouped F. graminearum in a separate cluster from the other species within the complex. However, AFLP analysis failed to resolve the species into distinct clades with partial clustering of F. meridionale, F. austroamericanum, F. asiaticum and F graminearum isolates.
description Members of the Fusarium graminearum species complex (FGSC), such as F. graminearum and F. asiaticum, are the main cause of fusarium head blight (FHB) of wheat and barley worldwide. In this study, 117 FGSC isolates obtained from commercial barley grain produced in Argentina (n = 43 isolates), Brazil (n = 35), and Uruguay (n = 39) were identified to species and trichothecene genotypes, and analysed using amplified fragment length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers. In addition, reductase (RED) and trichothecene 3-Oacetyltransferase (Tri101) were sequenced for a subset of 24 isolates. The majority of the isolates (n = 103) were identified as F. graminearum, which was the only species found in Argentina. In Uruguay, only one F. cortaderiae isolate was found among F. graminearum isolates. In Brazil, F. graminearum also dominated the collection (22/35), followed by F. meridionale (8/35), F. asiaticum (2/35), F. cortaderiae (2/35) and F. austroamericanum (1/35). Species were structured by trichothecene genotype: all F. graminearum were of the 15-acetyldeoxynivalenol (ADON), F. meridionale, F. asiaticum and F. cortaderiae were of the nivalenol (NIV), and F. austroamericanum was of the 3 ADON genotype. Both AFLP and SRAP data showed high levels of genetic variability, which was higher within than among countries. Isolates were not structured by country of origin. SRAP analysis grouped F. graminearum in a separate cluster from the other species within the complex. However, AFLP analysis failed to resolve the species into distinct clades with partial clustering of F. meridionale, F. austroamericanum, F. asiaticum and F graminearum isolates.
publishDate 2016
dc.date.none.fl_str_mv 2016
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