Development of Crystal Violet encapsulation in pectin

Autores
Revuelta, Mariana Valeria; Chacon Villalba, María Elizabeth; Navarro, Alba S.; Güida, Jorge A.; Castro, Guillermo Raúl
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Six pectins with 35% to 91% methoxylation degree were purified and characterized to encapsulate Crystal Violet (CV). Amidated low methoxylated pectin (ALMP) was selected based on microsphere morphologies, aqueous solubility, viscosity and the effect of calcium concentration. Pectin microspheres were stabilized with Arabic gum (AG) and optimized according to the loading. Microspheres composed of 2.0% ALMP-1.0% AG crosslinked with 450mMcalcium(II)were able to encapsulate 217±2 μMCV. Opticalmicroscopy of the gels revealed spheroid microspheres with 250 ± 50 μm diameter containing homogenous CV distribution. Dried microspheres observed by SEM and epifluorescence showed a highly shrinkable matrix keeping the spheroidal morphology. Low relative viscosity of the ALMP-AG-CV solutions was found compared to ALMP and ALMP-AG. The Young moduli (60–80 Pa) of ALMP-AG microspheres by texturometric analysis indicated that the CV could interfere with the gel crosslinking. Raman spectroscopy analysis suggested some interaction of CV nucleophilic center within the matrix. FTIR of the matrix showed largest shifts in the carbonyl and carboxylate groups probably associated to H-bridges. CV stability studies performed on ALMP-AG microspheres, synthetized from polymer solutions with pH values above and below pectin pKa and showed faster CV release rates in presence of ionic strength.
Materia
Ciencias Químicas
Crystal violet
microencapsulation
Ionotropic gelation
Vibrational spectroscopies
Rheological studies
Pectinas
Goma Arábiga
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/10224

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/10224
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Development of Crystal Violet encapsulation in pectinRevuelta, Mariana ValeriaChacon Villalba, María ElizabethNavarro, Alba S.Güida, Jorge A.Castro, Guillermo RaúlCiencias QuímicasCrystal violetmicroencapsulationIonotropic gelationVibrational spectroscopiesRheological studiesPectinasGoma ArábigaSix pectins with 35% to 91% methoxylation degree were purified and characterized to encapsulate Crystal Violet (CV). Amidated low methoxylated pectin (ALMP) was selected based on microsphere morphologies, aqueous solubility, viscosity and the effect of calcium concentration. Pectin microspheres were stabilized with Arabic gum (AG) and optimized according to the loading. Microspheres composed of 2.0% ALMP-1.0% AG crosslinked with 450mMcalcium(II)were able to encapsulate 217±2 μMCV. Opticalmicroscopy of the gels revealed spheroid microspheres with 250 ± 50 μm diameter containing homogenous CV distribution. Dried microspheres observed by SEM and epifluorescence showed a highly shrinkable matrix keeping the spheroidal morphology. Low relative viscosity of the ALMP-AG-CV solutions was found compared to ALMP and ALMP-AG. The Young moduli (60–80 Pa) of ALMP-AG microspheres by texturometric analysis indicated that the CV could interfere with the gel crosslinking. Raman spectroscopy analysis suggested some interaction of CV nucleophilic center within the matrix. FTIR of the matrix showed largest shifts in the carbonyl and carboxylate groups probably associated to H-bridges. CV stability studies performed on ALMP-AG microspheres, synthetized from polymer solutions with pH values above and below pectin pKa and showed faster CV release rates in presence of ionic strength.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/10224enginfo:eu-repo/semantics/altIdentifier/issn/1381-5148info:eu-repo/semantics/altIdentifier/doi/10.1016/j.reactfunctpolym.2016.07.002info:eu-repo/semantics/altIdentifier/hdl/10915/78939info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:43:41Zoai:digital.cic.gba.gob.ar:11746/10224Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:43:41.678CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Development of Crystal Violet encapsulation in pectin
title Development of Crystal Violet encapsulation in pectin
spellingShingle Development of Crystal Violet encapsulation in pectin
Revuelta, Mariana Valeria
Ciencias Químicas
Crystal violet
microencapsulation
Ionotropic gelation
Vibrational spectroscopies
Rheological studies
Pectinas
Goma Arábiga
title_short Development of Crystal Violet encapsulation in pectin
title_full Development of Crystal Violet encapsulation in pectin
title_fullStr Development of Crystal Violet encapsulation in pectin
title_full_unstemmed Development of Crystal Violet encapsulation in pectin
title_sort Development of Crystal Violet encapsulation in pectin
dc.creator.none.fl_str_mv Revuelta, Mariana Valeria
Chacon Villalba, María Elizabeth
Navarro, Alba S.
Güida, Jorge A.
Castro, Guillermo Raúl
author Revuelta, Mariana Valeria
author_facet Revuelta, Mariana Valeria
Chacon Villalba, María Elizabeth
Navarro, Alba S.
Güida, Jorge A.
Castro, Guillermo Raúl
author_role author
author2 Chacon Villalba, María Elizabeth
Navarro, Alba S.
Güida, Jorge A.
Castro, Guillermo Raúl
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Químicas
Crystal violet
microencapsulation
Ionotropic gelation
Vibrational spectroscopies
Rheological studies
Pectinas
Goma Arábiga
topic Ciencias Químicas
Crystal violet
microencapsulation
Ionotropic gelation
Vibrational spectroscopies
Rheological studies
Pectinas
Goma Arábiga
dc.description.none.fl_txt_mv Six pectins with 35% to 91% methoxylation degree were purified and characterized to encapsulate Crystal Violet (CV). Amidated low methoxylated pectin (ALMP) was selected based on microsphere morphologies, aqueous solubility, viscosity and the effect of calcium concentration. Pectin microspheres were stabilized with Arabic gum (AG) and optimized according to the loading. Microspheres composed of 2.0% ALMP-1.0% AG crosslinked with 450mMcalcium(II)were able to encapsulate 217±2 μMCV. Opticalmicroscopy of the gels revealed spheroid microspheres with 250 ± 50 μm diameter containing homogenous CV distribution. Dried microspheres observed by SEM and epifluorescence showed a highly shrinkable matrix keeping the spheroidal morphology. Low relative viscosity of the ALMP-AG-CV solutions was found compared to ALMP and ALMP-AG. The Young moduli (60–80 Pa) of ALMP-AG microspheres by texturometric analysis indicated that the CV could interfere with the gel crosslinking. Raman spectroscopy analysis suggested some interaction of CV nucleophilic center within the matrix. FTIR of the matrix showed largest shifts in the carbonyl and carboxylate groups probably associated to H-bridges. CV stability studies performed on ALMP-AG microspheres, synthetized from polymer solutions with pH values above and below pectin pKa and showed faster CV release rates in presence of ionic strength.
description Six pectins with 35% to 91% methoxylation degree were purified and characterized to encapsulate Crystal Violet (CV). Amidated low methoxylated pectin (ALMP) was selected based on microsphere morphologies, aqueous solubility, viscosity and the effect of calcium concentration. Pectin microspheres were stabilized with Arabic gum (AG) and optimized according to the loading. Microspheres composed of 2.0% ALMP-1.0% AG crosslinked with 450mMcalcium(II)were able to encapsulate 217±2 μMCV. Opticalmicroscopy of the gels revealed spheroid microspheres with 250 ± 50 μm diameter containing homogenous CV distribution. Dried microspheres observed by SEM and epifluorescence showed a highly shrinkable matrix keeping the spheroidal morphology. Low relative viscosity of the ALMP-AG-CV solutions was found compared to ALMP and ALMP-AG. The Young moduli (60–80 Pa) of ALMP-AG microspheres by texturometric analysis indicated that the CV could interfere with the gel crosslinking. Raman spectroscopy analysis suggested some interaction of CV nucleophilic center within the matrix. FTIR of the matrix showed largest shifts in the carbonyl and carboxylate groups probably associated to H-bridges. CV stability studies performed on ALMP-AG microspheres, synthetized from polymer solutions with pH values above and below pectin pKa and showed faster CV release rates in presence of ionic strength.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/10224
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dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1381-5148
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.reactfunctpolym.2016.07.002
info:eu-repo/semantics/altIdentifier/hdl/10915/78939
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
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institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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