Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells

Autores
McCarthy, Antonio Desmond; Etcheverry, Susana B.; Bruzzone, Liliana; Cortizo, Ana María
Año de publicación
1997
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.
Materia
Ciencias Químicas
advanced glycation endproducts
diabetes mellitus
osteoblastic cells
cell proliferation
cell differentiation
bone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4899
Acceder
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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/4899
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cellsMcCarthy, Antonio DesmondEtcheverry, Susana B.Bruzzone, LilianaCortizo, Ana MaríaCiencias Químicasadvanced glycation endproductsdiabetes mellitusosteoblastic cellscell proliferationcell differentiationboneTwo different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4899spainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-10-16T09:27:08Zoai:digital.cic.gba.gob.ar:11746/4899Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-10-16 09:27:08.606CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
title Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
spellingShingle Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
McCarthy, Antonio Desmond
Ciencias Químicas
advanced glycation endproducts
diabetes mellitus
osteoblastic cells
cell proliferation
cell differentiation
bone
title_short Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
title_full Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
title_fullStr Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
title_full_unstemmed Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
title_sort Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells
dc.creator.none.fl_str_mv McCarthy, Antonio Desmond
Etcheverry, Susana B.
Bruzzone, Liliana
Cortizo, Ana María
author McCarthy, Antonio Desmond
author_facet McCarthy, Antonio Desmond
Etcheverry, Susana B.
Bruzzone, Liliana
Cortizo, Ana María
author_role author
author2 Etcheverry, Susana B.
Bruzzone, Liliana
Cortizo, Ana María
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Químicas
advanced glycation endproducts
diabetes mellitus
osteoblastic cells
cell proliferation
cell differentiation
bone
topic Ciencias Químicas
advanced glycation endproducts
diabetes mellitus
osteoblastic cells
cell proliferation
cell differentiation
bone
dc.description.none.fl_txt_mv Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.
description Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.
publishDate 1997
dc.date.none.fl_str_mv 1997
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4899
url https://digital.cic.gba.gob.ar/handle/11746/4899
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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