Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)

Autores
Franco, Mario Emilio Ernesto; López, Silvina Marianela Yanil; Saparrat, Mario; Balatti, Pedro Alberto
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión enviada
Descripción
Pezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care.
Materia
Agronomía, reproducción y protección de plantas
Ascomycota
Mitochondrial genes
Phylogeny
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/8215

id CICBA_5a98e0ce74f978784462f021311b0fa8
oai_identifier_str oai:digital.cic.gba.gob.ar:11746/8215
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)Franco, Mario Emilio ErnestoLópez, Silvina Marianela YanilSaparrat, MarioBalatti, Pedro AlbertoAgronomía, reproducción y protección de plantasAscomycotaMitochondrial genesPhylogenyPezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care.2016-07-15info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8215enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:47Zoai:digital.cic.gba.gob.ar:11746/8215Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:47.833CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
title Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
spellingShingle Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
Franco, Mario Emilio Ernesto
Agronomía, reproducción y protección de plantas
Ascomycota
Mitochondrial genes
Phylogeny
title_short Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
title_full Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
title_fullStr Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
title_full_unstemmed Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
title_sort Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
dc.creator.none.fl_str_mv Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Saparrat, Mario
Balatti, Pedro Alberto
author Franco, Mario Emilio Ernesto
author_facet Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Saparrat, Mario
Balatti, Pedro Alberto
author_role author
author2 López, Silvina Marianela Yanil
Saparrat, Mario
Balatti, Pedro Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
Ascomycota
Mitochondrial genes
Phylogeny
topic Agronomía, reproducción y protección de plantas
Ascomycota
Mitochondrial genes
Phylogeny
dc.description.none.fl_txt_mv Pezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care.
description Pezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-15
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/8215
url https://digital.cic.gba.gob.ar/handle/11746/8215
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
_version_ 1844618576724492289
score 13.070432