Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)
- Autores
- Franco, Mario Emilio Ernesto; López, Silvina Marianela Yanil; Saparrat, Mario; Balatti, Pedro Alberto
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión enviada
- Descripción
- Pezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care.
- Materia
-
Agronomía, reproducción y protección de plantas
Ascomycota
Mitochondrial genes
Phylogeny - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/8215
Ver los metadatos del registro completo
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Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota)Franco, Mario Emilio ErnestoLópez, Silvina Marianela YanilSaparrat, MarioBalatti, Pedro AlbertoAgronomía, reproducción y protección de plantasAscomycotaMitochondrial genesPhylogenyPezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care.2016-07-15info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8215enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:47Zoai:digital.cic.gba.gob.ar:11746/8215Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:47.833CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
title |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
spellingShingle |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) Franco, Mario Emilio Ernesto Agronomía, reproducción y protección de plantas Ascomycota Mitochondrial genes Phylogeny |
title_short |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
title_full |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
title_fullStr |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
title_full_unstemmed |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
title_sort |
Mitochondrial and nuclear gene sequences to infer the phylogeny of Pezizomycotina (Ascomycota) |
dc.creator.none.fl_str_mv |
Franco, Mario Emilio Ernesto López, Silvina Marianela Yanil Saparrat, Mario Balatti, Pedro Alberto |
author |
Franco, Mario Emilio Ernesto |
author_facet |
Franco, Mario Emilio Ernesto López, Silvina Marianela Yanil Saparrat, Mario Balatti, Pedro Alberto |
author_role |
author |
author2 |
López, Silvina Marianela Yanil Saparrat, Mario Balatti, Pedro Alberto |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Agronomía, reproducción y protección de plantas Ascomycota Mitochondrial genes Phylogeny |
topic |
Agronomía, reproducción y protección de plantas Ascomycota Mitochondrial genes Phylogeny |
dc.description.none.fl_txt_mv |
Pezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care. |
description |
Pezizomycotina is the largest subphylum of Ascomycota. It includes filamentous species widespread in nature, where they live as saprotrophs, mutualists and/or parasites of animal, plants and fungi. We evaluated the phylogeny of such an important subphylum not only by analyzing the sequence of nuclear genes but also of the core mitochondrial ones, since this organelle may provide additional information. Hence, we compared the phylogenetic reconstitution provided by the concatenated nucleotide sequences of 12 core mitochondrial genes (atp6, cob, cox1-3, nad1-6 and nad4L) and the individual and concatenated nucleotide sequences of 5 widely used nuclear genes (β-tub, tef-1α, gpd, rpb1 and rpb2) of 28 Pezizomycotina species. Alignments were generated with ClustalW and automatically curated with Gblocks 0.91b. Best-fit model of evolution was selected with jModelTest 2.1.7 and data matrices were analyzed under maximum likelihood and bayesian criteria in PhyML 3.0 and MrBayes 3.2, respectively. We found that only rpb2 and the concatenated nuclear and mitochondrial datasets clustered species in accordance with the current systematic of this subphylum, although incongruences were observed in some internal nodes. Discrepancies between the remaining datasets are indicative of the complex evolutionary histories and confirmed that the genes for an accurate phylogenetic estimation should be chosen with special care. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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conferenceObject |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/8215 |
url |
https://digital.cic.gba.gob.ar/handle/11746/8215 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
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Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
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CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
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marisa.degiusti@sedici.unlp.edu.ar |
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