Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA

Autores
Ogara, M.F.; Sirkin, P.F.; Carcagno, A.L.; Marazita, M.C.; Sonzogni, S.V.; Ceruti, J.M.; Cánepa, E.T.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM/ATR, Chk1/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies. © 2013 Ogara et al.
Fil:Ogara, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Carcagno, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Marazita, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Sonzogni, S.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ceruti, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
PLoS ONE 2013;8(4)
Materia
ATM protein
ATR protein
checkpoint kinase 1
checkpoint kinase 2
cyclin dependent kinase inhibitor 2D
transcription factor E2F
article
cell cycle regulation
cell function
chromatin
chromatin relaxation
chromatin structure
controlled study
DNA repair
enzyme activity
genotoxicity
human
human cell
protein induction
signal transduction
Cell Cycle Proteins
Cell Line
Chloroquine
Chromatin
Cyclin-Dependent Kinase Inhibitor p19
DNA Damage
DNA Repair
DNA-Binding Proteins
E2F1 Transcription Factor
Humans
Models, Biological
Mutagens
Protein Kinases
Protein-Serine-Threonine Kinases
Signal Transduction
Tumor Suppressor Proteins
Ultraviolet Rays
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_19326203_v8_n4_p_Ogara

id BDUBAFCEN_d37f1eb3e909938bdd89604e45a25d70
oai_identifier_str paperaa:paper_19326203_v8_n4_p_Ogara
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNAOgara, M.F.Sirkin, P.F.Carcagno, A.L.Marazita, M.C.Sonzogni, S.V.Ceruti, J.M.Cánepa, E.T.ATM proteinATR proteincheckpoint kinase 1checkpoint kinase 2cyclin dependent kinase inhibitor 2Dtranscription factor E2Farticlecell cycle regulationcell functionchromatinchromatin relaxationchromatin structurecontrolled studyDNA repairenzyme activitygenotoxicityhumanhuman cellprotein inductionsignal transductionCell Cycle ProteinsCell LineChloroquineChromatinCyclin-Dependent Kinase Inhibitor p19DNA DamageDNA RepairDNA-Binding ProteinsE2F1 Transcription FactorHumansModels, BiologicalMutagensProtein KinasesProtein-Serine-Threonine KinasesSignal TransductionTumor Suppressor ProteinsUltraviolet RaysThe maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM/ATR, Chk1/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies. © 2013 Ogara et al.Fil:Ogara, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Carcagno, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Marazita, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Sonzogni, S.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ceruti, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_19326203_v8_n4_p_OgaraPLoS ONE 2013;8(4)reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:03Zpaperaa:paper_19326203_v8_n4_p_OgaraInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:04.601Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
spellingShingle Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
Ogara, M.F.
ATM protein
ATR protein
checkpoint kinase 1
checkpoint kinase 2
cyclin dependent kinase inhibitor 2D
transcription factor E2F
article
cell cycle regulation
cell function
chromatin
chromatin relaxation
chromatin structure
controlled study
DNA repair
enzyme activity
genotoxicity
human
human cell
protein induction
signal transduction
Cell Cycle Proteins
Cell Line
Chloroquine
Chromatin
Cyclin-Dependent Kinase Inhibitor p19
DNA Damage
DNA Repair
DNA-Binding Proteins
E2F1 Transcription Factor
Humans
Models, Biological
Mutagens
Protein Kinases
Protein-Serine-Threonine Kinases
Signal Transduction
Tumor Suppressor Proteins
Ultraviolet Rays
title_short Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_full Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_fullStr Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_full_unstemmed Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_sort Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
dc.creator.none.fl_str_mv Ogara, M.F.
Sirkin, P.F.
Carcagno, A.L.
Marazita, M.C.
Sonzogni, S.V.
Ceruti, J.M.
Cánepa, E.T.
author Ogara, M.F.
author_facet Ogara, M.F.
Sirkin, P.F.
Carcagno, A.L.
Marazita, M.C.
Sonzogni, S.V.
Ceruti, J.M.
Cánepa, E.T.
author_role author
author2 Sirkin, P.F.
Carcagno, A.L.
Marazita, M.C.
Sonzogni, S.V.
Ceruti, J.M.
Cánepa, E.T.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ATM protein
ATR protein
checkpoint kinase 1
checkpoint kinase 2
cyclin dependent kinase inhibitor 2D
transcription factor E2F
article
cell cycle regulation
cell function
chromatin
chromatin relaxation
chromatin structure
controlled study
DNA repair
enzyme activity
genotoxicity
human
human cell
protein induction
signal transduction
Cell Cycle Proteins
Cell Line
Chloroquine
Chromatin
Cyclin-Dependent Kinase Inhibitor p19
DNA Damage
DNA Repair
DNA-Binding Proteins
E2F1 Transcription Factor
Humans
Models, Biological
Mutagens
Protein Kinases
Protein-Serine-Threonine Kinases
Signal Transduction
Tumor Suppressor Proteins
Ultraviolet Rays
topic ATM protein
ATR protein
checkpoint kinase 1
checkpoint kinase 2
cyclin dependent kinase inhibitor 2D
transcription factor E2F
article
cell cycle regulation
cell function
chromatin
chromatin relaxation
chromatin structure
controlled study
DNA repair
enzyme activity
genotoxicity
human
human cell
protein induction
signal transduction
Cell Cycle Proteins
Cell Line
Chloroquine
Chromatin
Cyclin-Dependent Kinase Inhibitor p19
DNA Damage
DNA Repair
DNA-Binding Proteins
E2F1 Transcription Factor
Humans
Models, Biological
Mutagens
Protein Kinases
Protein-Serine-Threonine Kinases
Signal Transduction
Tumor Suppressor Proteins
Ultraviolet Rays
dc.description.none.fl_txt_mv The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM/ATR, Chk1/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies. © 2013 Ogara et al.
Fil:Ogara, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Carcagno, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Marazita, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Sonzogni, S.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ceruti, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM/ATR, Chk1/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies. © 2013 Ogara et al.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_19326203_v8_n4_p_Ogara
url http://hdl.handle.net/20.500.12110/paper_19326203_v8_n4_p_Ogara
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 2013;8(4)
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1844618738540740608
score 13.070432