New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon

Autores
Katzen, F.; Becker, A.; Ielmini, M.V.; Oddo, C.G.; Ielpi, L.
Año de publicación
1999
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Appl. Environ. Microbiol. 1999;65(1):278-282
Materia
xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00992240_v65_n1_p278_Katzen
Acceder
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oai_identifier_str paperaa:paper_00992240_v65_n1_p278_Katzen
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operonKatzen, F.Becker, A.Ielmini, M.V.Oddo, C.G.Ielpi, L.xanthanarticlebacterium identificationbiosynthesiscloning vectorcolorimetrycrossing overgene disruptiongene mappinggram negative bacteriumnonhumannucleotide sequenceoperonreverse transcription polymerase chain reactionsequence analysissite directed mutagenesisxanthomonasBase SequenceChromosome MappingDNA PrimersDNA, BacterialGenes, BacterialGenetic VectorsGram-Negative BacteriaMolecular Sequence DataMutagenesis, Site-DirectedOperonReverse Transcriptase Polymerase Chain ReactionXanthomonas campestrisBacteria (microorganisms)NegibacteriaXanthomonasXanthomonas campestrisXanthomonas campestris pv. campestrisWe describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_KatzenAppl. Environ. Microbiol. 1999;65(1):278-282reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-04T09:48:35Zpaperaa:paper_00992240_v65_n1_p278_KatzenInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-04 09:48:36.449Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
spellingShingle New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
Katzen, F.
xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
title_short New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_full New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_fullStr New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_full_unstemmed New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_sort New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
dc.creator.none.fl_str_mv Katzen, F.
Becker, A.
Ielmini, M.V.
Oddo, C.G.
Ielpi, L.
author Katzen, F.
author_facet Katzen, F.
Becker, A.
Ielmini, M.V.
Oddo, C.G.
Ielpi, L.
author_role author
author2 Becker, A.
Ielmini, M.V.
Oddo, C.G.
Ielpi, L.
author2_role author
author
author
author
dc.subject.none.fl_str_mv xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
topic xanthan
article
bacterium identification
biosynthesis
cloning vector
colorimetry
crossing over
gene disruption
gene mapping
gram negative bacterium
nonhuman
nucleotide sequence
operon
reverse transcription polymerase chain reaction
sequence analysis
site directed mutagenesis
xanthomonas
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Bacterial
Genes, Bacterial
Genetic Vectors
Gram-Negative Bacteria
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Reverse Transcriptase Polymerase Chain Reaction
Xanthomonas campestris
Bacteria (microorganisms)
Negibacteria
Xanthomonas
Xanthomonas campestris
Xanthomonas campestris pv. campestris
dc.description.none.fl_txt_mv We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
url http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Appl. Environ. Microbiol. 1999;65(1):278-282
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1842340704503726080
score 12.623145

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