Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α

• Autores: Dekanty, A.; Giulianelli, S.; Coso, O.A.; Rudland, P.S.; Jimenez de Asua, L.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: FEBS Lett. 2006;580(10):2512-2516
• Materia: Cyclin D1; ERK1-2; MAPK; p38MAPK; Prostaglandin F2α; S-phase; 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene; 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole; cyclin D1; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; prostaglandin F2 alpha; retinoblastoma protein; cyclin D1; mitogen activated protein kinase; prostaglandin F2 alpha; animal cell; article; cell cycle S phase; cell line; cell proliferation; controlled study; DNA synthesis; enzyme activation; enzyme induction; enzyme inhibition; gene expression; mouse; nonhuman; priority journal; protein expression; protein phosphorylation; signal transduction; animal; cell division; cell strain 3T3; DNA replication; drug effect; genetics; metabolism; polyacrylamide gel electrophoresis; Western blotting; Animalia; Animals; Blotting, Western; Cell Division; Cyclin D1; Dinoprost; DNA Replication; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Mice; Mitogen-Activated Protein Kinases; Swiss 3T3 Cells
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Prostaglandin F2α (PGF2α) induces cyclin D1 expression and DNA synthesis in Swiss 3T3 cells. In order to assess which signaling mechanisms are implicated in these processes, we have used both a pharmacological approach and interfering mutants. We demonstrate that PGF2α induces extracellular-signal-regulated kinase (ERK1-2) and p38MAPK activation, and inhibition of any of these signaling pathways completely blocks PGF2α-stimulated DNA synthesis. We also show that ERK1-2, but not p38MAPK activation is required to induce cyclin D1 expression, strongly suggesting that the concerted action of cyclin D1 gene expression and other events are required to induce complete phosphorylation of retinoblastoma protein and S-phase entry in response to PGF2α. © 2006.
Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Giulianelli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Jimenez de Asua, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.