A plasmid vector for isolation of strong promoters in Escherichia coli

Autores
Carbonelli, D.L.; Corley, E.; Seigelchifer, M.; Zorzópulos, J.
Año de publicación
1999
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.
Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
FEMS Microbiol. Lett. 1999;177(1):75-82
Materia
Bacterial promoter
Bacteriophage promoter
Promoter isolation
Strong promoter
Vector
ampicillin
DNA fragment
penicillinase
plasmid vector
tetracycline
antibiotic resistance
article
bacteriophage
DNA sequence
Escherichia coli
nonhuman
nucleotide sequence
penicillin resistance
plasmid
priority journal
promoter region
Staphylococcus aureus
Bacteria (microorganisms)
bacteriophage a
Escherichia coli
Escherichia coli
Negibacteria
Posibacteria
Prokaryota
Staphylococcus aureus
Staphylococcus aureus
unidentified bacteriophage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_03781097_v177_n1_p75_Carbonelli

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oai_identifier_str paperaa:paper_03781097_v177_n1_p75_Carbonelli
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling A plasmid vector for isolation of strong promoters in Escherichia coliCarbonelli, D.L.Corley, E.Seigelchifer, M.Zorzópulos, J.Bacterial promoterBacteriophage promoterPromoter isolationStrong promoterVectorampicillinDNA fragmentpenicillinaseplasmid vectortetracyclineantibiotic resistancearticlebacteriophageDNA sequenceEscherichia colinonhumannucleotide sequencepenicillin resistanceplasmidpriority journalpromoter regionStaphylococcus aureusBacteria (microorganisms)bacteriophage aEscherichia coliEscherichia coliNegibacteriaPosibacteriaProkaryotaStaphylococcus aureusStaphylococcus aureusunidentified bacteriophageIn order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_CarbonelliFEMS Microbiol. Lett. 1999;177(1):75-82reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:58Zpaperaa:paper_03781097_v177_n1_p75_CarbonelliInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:59.285Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv A plasmid vector for isolation of strong promoters in Escherichia coli
title A plasmid vector for isolation of strong promoters in Escherichia coli
spellingShingle A plasmid vector for isolation of strong promoters in Escherichia coli
Carbonelli, D.L.
Bacterial promoter
Bacteriophage promoter
Promoter isolation
Strong promoter
Vector
ampicillin
DNA fragment
penicillinase
plasmid vector
tetracycline
antibiotic resistance
article
bacteriophage
DNA sequence
Escherichia coli
nonhuman
nucleotide sequence
penicillin resistance
plasmid
priority journal
promoter region
Staphylococcus aureus
Bacteria (microorganisms)
bacteriophage a
Escherichia coli
Escherichia coli
Negibacteria
Posibacteria
Prokaryota
Staphylococcus aureus
Staphylococcus aureus
unidentified bacteriophage
title_short A plasmid vector for isolation of strong promoters in Escherichia coli
title_full A plasmid vector for isolation of strong promoters in Escherichia coli
title_fullStr A plasmid vector for isolation of strong promoters in Escherichia coli
title_full_unstemmed A plasmid vector for isolation of strong promoters in Escherichia coli
title_sort A plasmid vector for isolation of strong promoters in Escherichia coli
dc.creator.none.fl_str_mv Carbonelli, D.L.
Corley, E.
Seigelchifer, M.
Zorzópulos, J.
author Carbonelli, D.L.
author_facet Carbonelli, D.L.
Corley, E.
Seigelchifer, M.
Zorzópulos, J.
author_role author
author2 Corley, E.
Seigelchifer, M.
Zorzópulos, J.
author2_role author
author
author
dc.subject.none.fl_str_mv Bacterial promoter
Bacteriophage promoter
Promoter isolation
Strong promoter
Vector
ampicillin
DNA fragment
penicillinase
plasmid vector
tetracycline
antibiotic resistance
article
bacteriophage
DNA sequence
Escherichia coli
nonhuman
nucleotide sequence
penicillin resistance
plasmid
priority journal
promoter region
Staphylococcus aureus
Bacteria (microorganisms)
bacteriophage a
Escherichia coli
Escherichia coli
Negibacteria
Posibacteria
Prokaryota
Staphylococcus aureus
Staphylococcus aureus
unidentified bacteriophage
topic Bacterial promoter
Bacteriophage promoter
Promoter isolation
Strong promoter
Vector
ampicillin
DNA fragment
penicillinase
plasmid vector
tetracycline
antibiotic resistance
article
bacteriophage
DNA sequence
Escherichia coli
nonhuman
nucleotide sequence
penicillin resistance
plasmid
priority journal
promoter region
Staphylococcus aureus
Bacteria (microorganisms)
bacteriophage a
Escherichia coli
Escherichia coli
Negibacteria
Posibacteria
Prokaryota
Staphylococcus aureus
Staphylococcus aureus
unidentified bacteriophage
dc.description.none.fl_txt_mv In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.
Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli
url http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv FEMS Microbiol. Lett. 1999;177(1):75-82
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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