A plasmid vector for isolation of strong promoters in Escherichia coli
- Autores
- Carbonelli, D.L.; Corley, E.; Seigelchifer, M.; Zorzópulos, J.
- Año de publicación
- 1999
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.
Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- FEMS Microbiol. Lett. 1999;177(1):75-82
- Materia
-
Bacterial promoter
Bacteriophage promoter
Promoter isolation
Strong promoter
Vector
ampicillin
DNA fragment
penicillinase
plasmid vector
tetracycline
antibiotic resistance
article
bacteriophage
DNA sequence
Escherichia coli
nonhuman
nucleotide sequence
penicillin resistance
plasmid
priority journal
promoter region
Staphylococcus aureus
Bacteria (microorganisms)
bacteriophage a
Escherichia coli
Escherichia coli
Negibacteria
Posibacteria
Prokaryota
Staphylococcus aureus
Staphylococcus aureus
unidentified bacteriophage - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_03781097_v177_n1_p75_Carbonelli
Ver los metadatos del registro completo
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A plasmid vector for isolation of strong promoters in Escherichia coliCarbonelli, D.L.Corley, E.Seigelchifer, M.Zorzópulos, J.Bacterial promoterBacteriophage promoterPromoter isolationStrong promoterVectorampicillinDNA fragmentpenicillinaseplasmid vectortetracyclineantibiotic resistancearticlebacteriophageDNA sequenceEscherichia colinonhumannucleotide sequencepenicillin resistanceplasmidpriority journalpromoter regionStaphylococcus aureusBacteria (microorganisms)bacteriophage aEscherichia coliEscherichia coliNegibacteriaPosibacteriaProkaryotaStaphylococcus aureusStaphylococcus aureusunidentified bacteriophageIn order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_CarbonelliFEMS Microbiol. Lett. 1999;177(1):75-82reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:58Zpaperaa:paper_03781097_v177_n1_p75_CarbonelliInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:59.285Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
A plasmid vector for isolation of strong promoters in Escherichia coli |
title |
A plasmid vector for isolation of strong promoters in Escherichia coli |
spellingShingle |
A plasmid vector for isolation of strong promoters in Escherichia coli Carbonelli, D.L. Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage |
title_short |
A plasmid vector for isolation of strong promoters in Escherichia coli |
title_full |
A plasmid vector for isolation of strong promoters in Escherichia coli |
title_fullStr |
A plasmid vector for isolation of strong promoters in Escherichia coli |
title_full_unstemmed |
A plasmid vector for isolation of strong promoters in Escherichia coli |
title_sort |
A plasmid vector for isolation of strong promoters in Escherichia coli |
dc.creator.none.fl_str_mv |
Carbonelli, D.L. Corley, E. Seigelchifer, M. Zorzópulos, J. |
author |
Carbonelli, D.L. |
author_facet |
Carbonelli, D.L. Corley, E. Seigelchifer, M. Zorzópulos, J. |
author_role |
author |
author2 |
Corley, E. Seigelchifer, M. Zorzópulos, J. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage |
topic |
Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage |
dc.description.none.fl_txt_mv |
In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies. Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli |
url |
http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
FEMS Microbiol. Lett. 1999;177(1):75-82 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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1844618736740335616 |
score |
13.070432 |