Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
- Autores
- Briozzo, J.; Amato de Lagarde, E.; Chirife, J.; Parada, J.L.
- Año de publicación
- 1986
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.
Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- APPL. ENVIRON. MICROBIOL. 1986;51(4):844-848
- Materia
-
botulinum toxin
water
activity
bacterial growth
clostridium botulinum type g
nonhuman
ph
priority journal
Botulinum Toxins
Clostridium botulinum
Culture Media
Heat
Hydrogen-Ion Concentration
Support, Non-U.S. Gov't
Trypsin
Water - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00992240_v51_n4_p844_Briozzo
Ver los metadatos del registro completo
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network_name_str |
Biblioteca Digital (UBA-FCEN) |
spelling |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type GBriozzo, J.Amato de Lagarde, E.Chirife, J.Parada, J.L.botulinum toxinwateractivitybacterial growthclostridium botulinum type gnonhumanphpriority journalBotulinum ToxinsClostridium botulinumCulture MediaHeatHydrogen-Ion ConcentrationSupport, Non-U.S. Gov'tTrypsinWaterThe combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1986info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_BriozzoAPPL. ENVIRON. MICROBIOL. 1986;51(4):844-848reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:09Zpaperaa:paper_00992240_v51_n4_p844_BriozzoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:10.874Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
title |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
spellingShingle |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G Briozzo, J. botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water |
title_short |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
title_full |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
title_fullStr |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
title_full_unstemmed |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
title_sort |
Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G |
dc.creator.none.fl_str_mv |
Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. |
author |
Briozzo, J. |
author_facet |
Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. |
author_role |
author |
author2 |
Amato de Lagarde, E. Chirife, J. Parada, J.L. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water |
topic |
botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water |
dc.description.none.fl_txt_mv |
The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes. Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes. |
publishDate |
1986 |
dc.date.none.fl_str_mv |
1986 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo |
url |
http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
APPL. ENVIRON. MICROBIOL. 1986;51(4):844-848 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
_version_ |
1844618740881162240 |
score |
13.070432 |