Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G

Autores
Briozzo, J.; Amato de Lagarde, E.; Chirife, J.; Parada, J.L.
Año de publicación
1986
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.
Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
APPL. ENVIRON. MICROBIOL. 1986;51(4):844-848
Materia
botulinum toxin
water
activity
bacterial growth
clostridium botulinum type g
nonhuman
ph
priority journal
Botulinum Toxins
Clostridium botulinum
Culture Media
Heat
Hydrogen-Ion Concentration
Support, Non-U.S. Gov't
Trypsin
Water
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00992240_v51_n4_p844_Briozzo

id BDUBAFCEN_850901a2e7a146edad0dadcaa6e69750
oai_identifier_str paperaa:paper_00992240_v51_n4_p844_Briozzo
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Effect of water activity and pH on growth and toxin production by Clostridium botulinum type GBriozzo, J.Amato de Lagarde, E.Chirife, J.Parada, J.L.botulinum toxinwateractivitybacterial growthclostridium botulinum type gnonhumanphpriority journalBotulinum ToxinsClostridium botulinumCulture MediaHeatHydrogen-Ion ConcentrationSupport, Non-U.S. Gov'tTrypsinWaterThe combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1986info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_BriozzoAPPL. ENVIRON. MICROBIOL. 1986;51(4):844-848reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:09Zpaperaa:paper_00992240_v51_n4_p844_BriozzoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:10.874Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
spellingShingle Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
Briozzo, J.
botulinum toxin
water
activity
bacterial growth
clostridium botulinum type g
nonhuman
ph
priority journal
Botulinum Toxins
Clostridium botulinum
Culture Media
Heat
Hydrogen-Ion Concentration
Support, Non-U.S. Gov't
Trypsin
Water
title_short Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_full Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_fullStr Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_full_unstemmed Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_sort Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
dc.creator.none.fl_str_mv Briozzo, J.
Amato de Lagarde, E.
Chirife, J.
Parada, J.L.
author Briozzo, J.
author_facet Briozzo, J.
Amato de Lagarde, E.
Chirife, J.
Parada, J.L.
author_role author
author2 Amato de Lagarde, E.
Chirife, J.
Parada, J.L.
author2_role author
author
author
dc.subject.none.fl_str_mv botulinum toxin
water
activity
bacterial growth
clostridium botulinum type g
nonhuman
ph
priority journal
Botulinum Toxins
Clostridium botulinum
Culture Media
Heat
Hydrogen-Ion Concentration
Support, Non-U.S. Gov't
Trypsin
Water
topic botulinum toxin
water
activity
bacterial growth
clostridium botulinum type g
nonhuman
ph
priority journal
Botulinum Toxins
Clostridium botulinum
Culture Media
Heat
Hydrogen-Ion Concentration
Support, Non-U.S. Gov't
Trypsin
Water
dc.description.none.fl_txt_mv The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.
Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.
publishDate 1986
dc.date.none.fl_str_mv 1986
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo
url http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv APPL. ENVIRON. MICROBIOL. 1986;51(4):844-848
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1844618740881162240
score 13.070432