Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii

Autores
Gómez, G.A.; Hasson, E.
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.
Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Mol. Biol. Evol. 2003;20(3):410-423
Materia
Drosophila buzzatii
Esterase-A
Gene flow
Inversion polymorphism
Natural selection
Nucleotide variation
esterase
amino acid substitution
animal experiment
article
DNA polymorphism
Drosophila
drosophila buzzatii
female
gene conversion
gene frequency
gene mapping
gene rearrangement
genetic code
genetic linkage
genetic variability
nonhuman
nucleotide sequence
polymorphic locus
species differentiation
theory
Animals
Base Sequence
DNA
DNA Primers
Drosophila
Gene Rearrangement
In Situ Hybridization
Linkage (Genetics)
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Recombination, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
Variation (Genetics)
Animalia
Arachnida
Drosophila buzzatii
Drosophila koepferae
Hexapoda
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_07374038_v20_n3_p410_Gomez

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oai_identifier_str paperaa:paper_07374038_v20_n3_p410_Gomez
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatiiGómez, G.A.Hasson, E.Drosophila buzzatiiEsterase-AGene flowInversion polymorphismNatural selectionNucleotide variationesteraseamino acid substitutionanimal experimentarticleDNA polymorphismDrosophiladrosophila buzzatiifemalegene conversiongene frequencygene mappinggene rearrangementgenetic codegenetic linkagegenetic variabilitynonhumannucleotide sequencepolymorphic locusspecies differentiationtheoryAnimalsBase SequenceDNADNA PrimersDrosophilaGene RearrangementIn Situ HybridizationLinkage (Genetics)Molecular Sequence DataPolymerase Chain ReactionPolymorphism, GeneticRecombination, GeneticSequence AlignmentSequence Homology, Nucleic AcidVariation (Genetics)AnimaliaArachnidaDrosophila buzzatiiDrosophila koepferaeHexapodaNucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_GomezMol. Biol. Evol. 2003;20(3):410-423reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:09Zpaperaa:paper_07374038_v20_n3_p410_GomezInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:10.298Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
spellingShingle Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
Gómez, G.A.
Drosophila buzzatii
Esterase-A
Gene flow
Inversion polymorphism
Natural selection
Nucleotide variation
esterase
amino acid substitution
animal experiment
article
DNA polymorphism
Drosophila
drosophila buzzatii
female
gene conversion
gene frequency
gene mapping
gene rearrangement
genetic code
genetic linkage
genetic variability
nonhuman
nucleotide sequence
polymorphic locus
species differentiation
theory
Animals
Base Sequence
DNA
DNA Primers
Drosophila
Gene Rearrangement
In Situ Hybridization
Linkage (Genetics)
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Recombination, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
Variation (Genetics)
Animalia
Arachnida
Drosophila buzzatii
Drosophila koepferae
Hexapoda
title_short Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_full Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_fullStr Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_full_unstemmed Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_sort Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
dc.creator.none.fl_str_mv Gómez, G.A.
Hasson, E.
author Gómez, G.A.
author_facet Gómez, G.A.
Hasson, E.
author_role author
author2 Hasson, E.
author2_role author
dc.subject.none.fl_str_mv Drosophila buzzatii
Esterase-A
Gene flow
Inversion polymorphism
Natural selection
Nucleotide variation
esterase
amino acid substitution
animal experiment
article
DNA polymorphism
Drosophila
drosophila buzzatii
female
gene conversion
gene frequency
gene mapping
gene rearrangement
genetic code
genetic linkage
genetic variability
nonhuman
nucleotide sequence
polymorphic locus
species differentiation
theory
Animals
Base Sequence
DNA
DNA Primers
Drosophila
Gene Rearrangement
In Situ Hybridization
Linkage (Genetics)
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Recombination, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
Variation (Genetics)
Animalia
Arachnida
Drosophila buzzatii
Drosophila koepferae
Hexapoda
topic Drosophila buzzatii
Esterase-A
Gene flow
Inversion polymorphism
Natural selection
Nucleotide variation
esterase
amino acid substitution
animal experiment
article
DNA polymorphism
Drosophila
drosophila buzzatii
female
gene conversion
gene frequency
gene mapping
gene rearrangement
genetic code
genetic linkage
genetic variability
nonhuman
nucleotide sequence
polymorphic locus
species differentiation
theory
Animals
Base Sequence
DNA
DNA Primers
Drosophila
Gene Rearrangement
In Situ Hybridization
Linkage (Genetics)
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Recombination, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
Variation (Genetics)
Animalia
Arachnida
Drosophila buzzatii
Drosophila koepferae
Hexapoda
dc.description.none.fl_txt_mv Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.
Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez
url http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Mol. Biol. Evol. 2003;20(3):410-423
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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