Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
- Autores
- Gómez, G.A.; Hasson, E.
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.
Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Mol. Biol. Evol. 2003;20(3):410-423
- Materia
-
Drosophila buzzatii
Esterase-A
Gene flow
Inversion polymorphism
Natural selection
Nucleotide variation
esterase
amino acid substitution
animal experiment
article
DNA polymorphism
Drosophila
drosophila buzzatii
female
gene conversion
gene frequency
gene mapping
gene rearrangement
genetic code
genetic linkage
genetic variability
nonhuman
nucleotide sequence
polymorphic locus
species differentiation
theory
Animals
Base Sequence
DNA
DNA Primers
Drosophila
Gene Rearrangement
In Situ Hybridization
Linkage (Genetics)
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Recombination, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
Variation (Genetics)
Animalia
Arachnida
Drosophila buzzatii
Drosophila koepferae
Hexapoda - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_07374038_v20_n3_p410_Gomez
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paperaa:paper_07374038_v20_n3_p410_Gomez |
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network_name_str |
Biblioteca Digital (UBA-FCEN) |
spelling |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatiiGómez, G.A.Hasson, E.Drosophila buzzatiiEsterase-AGene flowInversion polymorphismNatural selectionNucleotide variationesteraseamino acid substitutionanimal experimentarticleDNA polymorphismDrosophiladrosophila buzzatiifemalegene conversiongene frequencygene mappinggene rearrangementgenetic codegenetic linkagegenetic variabilitynonhumannucleotide sequencepolymorphic locusspecies differentiationtheoryAnimalsBase SequenceDNADNA PrimersDrosophilaGene RearrangementIn Situ HybridizationLinkage (Genetics)Molecular Sequence DataPolymerase Chain ReactionPolymorphism, GeneticRecombination, GeneticSequence AlignmentSequence Homology, Nucleic AcidVariation (Genetics)AnimaliaArachnidaDrosophila buzzatiiDrosophila koepferaeHexapodaNucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_GomezMol. Biol. Evol. 2003;20(3):410-423reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:09Zpaperaa:paper_07374038_v20_n3_p410_GomezInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:10.298Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
title |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
spellingShingle |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii Gómez, G.A. Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda |
title_short |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
title_full |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
title_fullStr |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
title_full_unstemmed |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
title_sort |
Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii |
dc.creator.none.fl_str_mv |
Gómez, G.A. Hasson, E. |
author |
Gómez, G.A. |
author_facet |
Gómez, G.A. Hasson, E. |
author_role |
author |
author2 |
Hasson, E. |
author2_role |
author |
dc.subject.none.fl_str_mv |
Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda |
topic |
Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda |
dc.description.none.fl_txt_mv |
Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez |
url |
http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Mol. Biol. Evol. 2003;20(3):410-423 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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1844618739932200960 |
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13.070432 |